Enhanced ouabain resistance gene as a eukaryotic selection marker

Human Gene Therapy

Volumn 13, Issue 8, 2002, Pages 969-977

  Aints, Alar ^a   Belusa, Roger ^a   Andersson, Ronnie M ^a   Güven, Hayrettin ^a   Dilber, M Sirac ^a  

^a KAROLINSKA UNIVERSITY HOSPITAL   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE TRIPHOSPHATASE (POTASSIUM SODIUM); GREEN FLUORESCENT PROTEIN; OUABAIN; PHOTOPROTEIN; CYSTEINE; LEUCINE; RETROVIRUS VECTOR; RUBIDIUM 86;

AMINO ACID SUBSTITUTION; ANIMAL; ARTICLE; CELL CULTURE; DOG; DRUG ANTAGONISM; DRUG RESISTANCE; EUKARYOTIC CELL; GENE TRANSFER; GENE VECTOR; GENETIC MARKER; GENETICS; HELA CELL; HUMAN; METABOLISM; MONONUCLEAR CELL; MUTATION; POLYMERASE CHAIN REACTION; RAT; RETROVIRUS; SWINE; AMINO ACID ANALYSIS; CELL ASSAY; CELL POPULATION; CELL SELECTION; CONTROLLED STUDY; DERIVATIZATION; DNA MODIFICATION; EUKARYOTE; GENE; GENETIC ANALYSIS; GENETIC CODE; GENETIC SELECTION; GENETIC TRANSFECTION; HUMAN CELL; MOLECULAR BIOLOGY; MUTAGENESIS; OUABAIN RESISTANCE GENE; PLASMID; TARGET CELL; TIME;

AMINO ACID SUBSTITUTION; ANIMALS; CELLS, CULTURED; DOGS; DRUG RESISTANCE; EUKARYOTIC CELLS; GENE TRANSFER TECHNIQUES; GENETIC MARKERS; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; HELA CELLS; HUMANS; LEUKOCYTES, MONONUCLEAR; LUMINESCENT PROTEINS; MUTATION; NA(+)-K(+)-EXCHANGING ATPASE; OUABAIN; POLYMERASE CHAIN REACTION; RATS; RETROVIRIDAE; SWINE; ANIMAL; HUMAN; SUPPORT, NON-U.S. GOV'T;

EUKARYOTA; STROPHANTHUS GRATUS; UNIDENTIFIED RETROVIRUS;

EID: 0036000332     PISSN: 10430342     EISSN: None     Source Type: Journal    
DOI: 10.1089/10430340252939078     Document Type: Article

References (34)

1. +-ATPase activity influence cell attachment to fibronectin
2. Random mutagenesis of the sheep Na, K-ATPase α-1 subunit generates a novel T797N mutation that results in a ouabain-resistant enzyme
3. 1 subunit generating the ouabain-resistant mutant L793P
4. The selectable marker neo gene down-regulates gene expression from retroviral vectors containing an internal ribosome entry site
5. 1-subunit of the Na, K-AT-Pase with a T797-I797 substitution
6. Ex vivo T lymphocyte expansion for retroviral transduction: Influence of serum-free media on variations in cell expansion rates and lymphocyte subset distribution
7. Cytolytic T cell reactivity to Epstein-Barr virus is lost during in vitro T cell expansion
8. Measuring gene-transfer efficiency
9. +-ATPase α subunit identifies known and previously unidentified amino acid residues that alter ouabain sensitivity - Implications for ouabain binding
10. + cells transduced with retroviral vectors containing an intracytoplasmatically truncated version of the human low-affinity nerve growth factor receptor (ΔLNGFR) gene
11. CD34 splice variant: An attractive marker for selection of gene-modified cells
12. Analysis of amino acid residues in the H5-H6 transmembrane and extracellular domains of Na, K-ATPase α subunit identifies threonine 797 as a determinant of ouabain sensitivity
13. High-efficiency gene transfer and selection of human hematopoietic progenitor cells with a hybrid EBV/retroviral vector expressing the green fluorescence protein
14. FMEV vectors: Both retroviral long terminal repeat and leader are important for high expression in transduced hematopoietic cells
15. Design of 5′ untranslated sequences in retroviral vectors developed for medical use
16. Site-directed mutagenesis of the Na, K-ATPase: Consequences of substitutions of negatively-charged amino acids localized in the transmembrane domains
17. Episomal vectors rapidly and stably produce high-titer recombinant retrovirus
18. Asp804 and Asp808 in the transmembrane domain of the Na, K-ATPase α subunit are cation coordinating residues
19. In vivo drug-selectable genes: A new concept in gene therapy
20. Structure-function studies of the Na, K-ATPase
21. Cation and cardiac glycoside binding sites of the Na, K-ATPase
22. Ligand binding sites of Na, K-ATPase
23. Peripheral blood lymphocytes as target cells of retroviral vector-mediated gene transfer
24. Viral vectors for gene therapy of hematopoietic cells
25. Improved retroviral vectors for gene transfer and expression
26. Fluorescence-activated cell analysis and sorting of viable mammalian cells based on β-D-galactosidase activity after transduction of Escherichia coli lacZ
27. Ouabain interactions with the H5-H6 hairpin of the Na, K-ATPase reveal a possible inhibition mechanism via the cation binding domain
28. Selection of retrovirally transduced hematopoietic cells using CD24 as a marker of gene transfer
29. A new reporter system for detection of retroviral infection
30. + hematopoietic cells
31. Transfection of Na, K-ATPase α-subunit: Regulation of enzyme abundance
32. +)-ATPase
33. Characterization of the fourth a isoform of the Na, K-ATPase