Isolation and molecular characterization of a surface-bound proteinase of Entamoeba histolytica

Molecular Microbiology

Volumn 27, Issue 2, 1998, Pages 269-276

  Jacobs, Thomas ^a,c   Bruchhaus, Iris ^a   Dandekar, Thomas ^b   Tannich, Egbert ^a   Leippe, Matthias ^a  

^a BERNHARD NOCHT INSTITUTE FOR TROPICAL MEDICINE   (Germany)

^b EUROPEAN MOLECULAR BIOLOGY LABORATORY   (Germany)

^c HELMHOLTZ CENTRE FOR INFECTION RESEARCH   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

MEMBRANE PROTEIN; PROTEINASE; SCLEROPROTEIN;

ARTICLE; CYTOPATHOGENIC EFFECT; ENTAMOEBA HISTOLYTICA; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME ISOLATION; ENZYME PURIFICATION; IMMUNOCYTOCHEMISTRY; IN VITRO STUDY; MOLECULAR CLONING; PRIORITY JOURNAL;

AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; CYSTEINE ENDOPEPTIDASES; DNA, PROTOZOAN; ENTAMOEBA HISTOLYTICA; MEMBRANE PROTEINS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; PROTEIN CONFORMATION; RABBITS;

BACTERIA (MICROORGANISMS); ENTAMOEBA DISPAR; ENTAMOEBA HISTOLYTICA; PROTOZOA;

EID: 0031962434     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2958.1998.00662.x     Document Type: Article

References (35)

1. Avila, E.E., and Calderón, J. (1993) Entamoeba histolytica: a surface-associated cysteine protease. Exp Parasitol 76: 232-241.
2. Berti, P.J., and Storer, A.C. (1995) Alignment/phylogeny of the papain superfamily of cysteine proteases. J Mol Biol 246: 273-283.
3. Bracha, R., and Mirelman, D. (1984) Virulence of Entamoeba histolytica trophozoites. Effects of bacteria, microaerobic conditions and Metronidazole. J Exp Med 160: 353-368.
4. Bruchhaus, I., and Tannich, E. (1996) A gene highly homologous to ACP1 encoding cysteine proteinase 3 in Entamoeba histolytica is present and expressed in Entamoeba dispar. Parasitol Res 82: 189-192.
5. Bruchhaus, I., Jacobs, T., Leippe, M., and Tannich, E. (1996) Entamoeba histolytica and Entamoeba dispar: differences in numbers and expression of cysteine proteinase genes. Mol Microbiol 22: 255-263.
6. Chelvanayagam, G., and McKeaig, L. (1991) Stereo viewing on the PC/AT with EGA graphics. J Mol Graphics 9: 111-114.
7. Clos, J., and Brandau, S. (1994) pJC20 and pJC40 - two high-copy number vectors for T7RNA polymerase-dependent expression of recombinant genes in Escherichia coli. Protein Expression Purif 5: 133-137.
8. Devereux, J., Haeberli, P., and Smithies, O. (1984) A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res 12: 387-395.
9. Diamond, L.S., and Clark, C.G. (1993) A rediscription of Entamoeba histolytica Schaudinn, 1902 (emended Walker, 1911) separating it from Entamoeba dispar Brumpt 1925. J Euk Microbiol 40: 340-344.
10. Diamond, L.S., Harlow, D.R., and Cunnick, C.C. (1978) A new medium for axenic cultivation of Entamoeba histolytica and other Entamoeba. Trans R Soc Trop Med Hyg 72: 431-432.
11. Frohman, M.A., Dush, M.K., and Martin, G.R. (1988) Rapid production of full length cDNA from rare transcripts: Amplification using a single gene-specific oligonucleotide primer. Proc Natl Acad Sci USA 85: 8998-9002.
12. Higgins, D.G., Thompson, J.D., and Gibson, T.J. (1996) Using CLUSTAL for multiple sequence alignments. Methods Enzymol 260: 383-402.
13. Horstmann, R.D., Leippe, M., and Tannich, E. (1992) Recent progress in molecular biology of Entamoeba histolytica. Trop Med Parasitol 43: 213-218.
14. Keene, W.E., Petitt, M.G., Allen, S., and McKerrow, J.H. (1986) The major neutral proteinase of Entamoeba histolytica. J Exp Med 163: 536-549.
15. Kyte, J., and Doolittle, R.F. (1982) A simple method for displaying the hydropathic character of a protein. J Mol Biol 157: 105-132.
16. Leippe, M. (1997) Amoebapores. Parasitol Today 13: 178-183.
17. Leippe, M., Ebel, S., Schoenberger, O.L., Horstmann, R.D., and Müller- Eberhard, H.J. (1991) Pore-forming peptide of pathogenic Entamoeba histolytica. Proc Natl Acad Sci USA 88: 7659-7663.
18. Leippe, M., Tannich, E., Nickel, R., van der Goot, G., Pattus, F., Horstmann, R.D., and Müller-Eberhard, H.J. (1992) Primary and secondary structure of the pore-forming peptide of pathogenic Entamoeba histolytica. EMBO J 11: 3501-3506.
19. Leippe, M., Sievertsen, H.J., Tannich, E., and Horstmann, R.D. (1995) Spontaneous release of cysteine proteinases but not of pore-forming peptides by viable Entamoeba histolytica. Parasitology 111: 569-574.
20. Meesters, F., Shaw, E., Scholze, H., Stolarski, T., and Mirelman, D. (1990) Specific labelling of cysteine proteinases in pathogenic and non-pathogenic Entamoeba histolytica. Infect Immun 58: 1396-1401.
21. Mirelman, D., Nuchamowitz, Y., Böhm-Gloning, B., and Walderich, B. (1996) A homologue of the cysteine proteinase gene (ACP1 or EhCPp3) of pathogenic Entamoeba histolytica is present in Entamoeba dispar. Mol Biochem Parasitol 78: 47-54.
22. Munõz, M. de, L., Calderón, J., and Rojkind, M. (1982) The collagenase of Entamoeba histolytica. J Exp Med 155: 42-51.
23. Ostoa-Saloma, P., Cabrera, N., Becker, I., and Perez-Montfort, R. (1989) Proteinases of Entamoeba histolytica associated with different subcellular fractions. Mol Biochem Parasitol 32: 133-144.
24. Ravdin, J.I. (1995) Amebiasis. Clin Infect Dis 20: 1453-1466.
25. Reed, S., Bouvier, J., Pollack, A.S., Engel, J.C., Brown, M., Hirata, K., Que, X., Eakin, A., Hagblom, P., Gillin, F., and McKerrow, J.H. (1993) Cloning of a virulence factor of Entamoeba histolytica. J Clin Invest 91: 1532-1540.
26. Sayle, R.A., and Milner-White, E.J. (1995) RASMOL: Biomolecular graphics for all. Trends Biochem Sci. 20: 374.
27. Scholze, H., and Schulte, W. (1988) On the specificity of a cysteine proteinase from Entamoeba histolytica. Biomed Biochim Acta 47: 115-123.
28. Scholze, H., and Tannich, E. (1994) Cysteine endopeptidases of Entamoeba histolytica. Methods Enzymol 244: 512-523.
29. Smith, P.K., Krohn, R.I., Hermanson, G.T., Mallia, A.K., Gartner, F.H., Provenzano, M.D., Fujimoto, E.K., Goeke, N.M., Olson, B.J., and Klenk, D.C. (1985) Measurement of protein using bicinchoninic acid. Anal Biochem 150: 76-85.
30. Stanley, S.L. Jr., Zhang, T., Rubin, D., and Li, E. (1995) Role of the Entamoeba histolytica cysteine proteinase in amebic liver abscess formation in servere combined immunodeficient mice. Infect Immun 63: 1587-1590.
31. Tannich, E. (1996) Recent advances in DNA-mediated gene transfer in Entamoeba histolytica. Parasitol Today 12: 198-200.
32. Tannich, E., Scholze, H., Nickel, R., and Horstmann, R.D. (1991a) Homologous cysteine proteinase of pathogenic and non-pathogenic Entamoeba histolytica: differences in structure and expression. J Biol Chem 266: 4798-4803.
33. Tannich, E., Ebert, F., and Horstmann, R.D. (1991b) Primary structure of the 170 kDa surface lectin of pathogenic Entamoeba histolytica. Proc Natl Acad Sci USA 88: 1849-1853.
34. Tannich, E., Nickel, R., Buss, H., and Horstmann, R.D. (1992) Mapping and partial sequencing of the genes coding for two different cysteine proteinases in pathogenic Entamoeba histolytica. Mol Biochem Parasitol 54: 109-112.
35. Walsh, J.A. (1986) Problems in recognition and diagnosis of amebiasis: estimation of the global magnitude of morbidity and mortality. Rev Infect Dis 8: 228-238.