PCR-based ordered genomic libraries: A new approach to drug target identification for Streptococcus pneumoniae

Antimicrobial Agents and Chemotherapy

Volumn 46, Issue 8, 2002, Pages 2507-2512

  Belanger, Aimee E ^a   Lai, Angel ^a   Brackman, Marcia A ^a   LeBlanc, Donald J ^a  

^a ELI LILLY AND COMPANY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL DNA; ELONGATION FACTOR G; FUSIDIC ACID; ANTIINFECTIVE AGENT;

AMPLICON; ANTIBIOTIC RESISTANCE; ARTICLE; BIOINFORMATICS; DRUG BINDING; DRUG TARGETING; GENE IDENTIFICATION; GENE LIBRARY; MUTATION; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SEQUENCE ANALYSIS; STREPTOCOCCUS PNEUMONIAE; BIOLOGY; DRUG DESIGN; DRUG EFFECT; DRUG SCREENING; GENETICS; METHODOLOGY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SYNTHESIS;

ANTI-INFECTIVE AGENTS; ANTIBIOTICS; COMPUTATIONAL BIOLOGY; DNA, BACTERIAL; DRUG DESIGN; DRUG EVALUATION, PRECLINICAL; DRUG RESISTANCE, MICROBIAL; FUSIDIC ACID; GENE LIBRARY; MUTATION; PEPTIDE ELONGATION FACTOR G; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; STREPTOCOCCUS PNEUMONIAE; SUPPORT, NON-U.S. GOV'T; ANTI-BACTERIAL AGENTS;

EID: 0035991851     PISSN: 00664804     EISSN: None     Source Type: Journal    
DOI: 10.1128/AAC.46.8.2507-2512.2002     Document Type: Article

References (25)

1. Analysis of mutations using PCR and denaturing gradient gel electrophoresis
2. Antimicrobial resistance with Streptococcus pneumoniae in the United States, 1997-1998
3. 'Touchdown' PCR to circumvent spurious priming during gene amplification
4. Annotated draft sequence from a Streptococcus pneumoniae type 19F clinical isolate
5. Molecular evolution of rifampicin resistance in Streptococcus pneumoniae
6. The epidemiology of pneumococcal infection in children in the developing world
7. Penicillin-binding proteins in β-lactam-resistant Streptococcus pneumoniae
8. Genome of the bacterium Streptococcus pneumoniae strain R6
9. New mutation in ParE in a pneumococcal in vitro mutant resistant to fluoroquinolones
10. Fusidic acid-resistant mutants define three regions in elongation factor G of Salmonella typhimurium
11. Fidelity of DNA polymerases in DNA amplification
12. Pneumococcal disease and the role of conjugate vaccines
13. Cloning and expression of pneumococcal genes in Streptococcus pneumoniae
14. Relation between the transforming activity of a marker and its proximity to the end of the DNA particle
15. Structure of a mutant EF-G reveals domain III and possibly the fusidic acid binding site
16. Optimization of the polymerase chain reaction with regard to fidelity: Modified T7, Taq, and Vent DNA polymerases
17. Molecular basis of rifampin resistance in Streptococcus pneumoniae
18. Regulation of competence for genetic transformation in Streptococcus pneumoniae by an auto-induced peptide pheromone and a two-component regulatory system
19. A conservative amino acid mutation in the chromosome-encoded dihydrofolate reductase confers trimethoprim resistance in Streptococcus pneumoniae
20. The structure of ribosomal protein S5 reveals sites of interaction with 16S rRNA
21. Optimization and troubleshooting in PCR
22. The high level streptomycin resistance gene from Streptococcus pneumoniae is a homolog of the ribosomal protein S12 gene from Escherichia coli
23. Complete genome sequence of a virulent isolate of Streptococcus pneumoniae
24. Fidelity of DNA synthesis by the Thermus aquaticus DNA polymerase
25. The pneumococcus at the gates