Structure of a mutant EF-G reveals domain III and possibly the fusidic acid binding site

Journal of Molecular Biology

Volumn 303, Issue 4, 2000, Pages 593-603

  Laurberg, Martin ^a   Kristensen, Ole ^a   Martemyanov, Kirill ^b   Gudkov, Anatoly T ^b   Nagaev, Ivan ^c   Hughes, Diarmaid ^c   Liljas, Anders ^a  

^a LUND UNIVERSITY   (Sweden)

^b INSTITUTE OF PROTEIN RESEARCH   (Russian Federation)

^c UPPSALA UNIVERSITY   (Sweden)

Author keywords

Conformational change; Crystal structure; Elongation factor G; Fusidic acid resistance; Proteins synthesis

Indexed keywords

ELONGATION FACTOR; FUSIDIC ACID;

ARTICLE; BINDING SITE; CONTROLLED STUDY; CRYSTAL STRUCTURE; NONHUMAN; POINT MUTATION; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN STRUCTURE; PROTEIN SYNTHESIS; STRUCTURE ANALYSIS; THERMUS THERMOPHILUS;

THERMUS THERMOPHILUS;

EID: 0034602449     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.2000.4168     Document Type: Article

References (49)

1. Structure-based sequence alignment of elongation factors Tu and G with related GTPases involved in translation
2. Three-dimensional structure of the ribosomal translocase: Elongation factor G from Thermus thermophilus
3. Structure of the S15,S6,S18-rRNA complex: Assembly of the 30 S ribosome central domain
4. Visualization of elongation factor G on the Escherichia coli: 70 S ribosome: The mechanism of translocation
5. Effect of buffer conditions on the position of tRNA on the 70 S ribosome as visualized by cryoelectron microscopy
6. The structure of elongation factor G in complex with GDP: Conformational flexibility and nucleotide exchange
7. Ribosomal proteins and their structural transitions on and off the ribosome
8. Placement of protein and RNA structures into a 5 Å-resolution map of the 50 S ribosomal subunit
9. ALSCRIPT: A tool to format multiple sequence alignments
10. Crystal structure of active elongation factor Tu reveals major domain rearrangements
11. Crystallography and NMR system: A new software suite for macromolecular structure determination
12. Conserved structures and diversity of functions of RNA-binding proteins
13. A resolution of conflicting reports concerning the mode of action of fusidic acid
14. Ribbons
15. The CCP4 suite: Programs for protein crystallography
16. The conformational properties of elongation factor G and the mechanism of translocation
17. The crystal structure of elongation factor G complexed with GDP, at 2.7 Å resolution
18. Themes in RNA-protein recognition
19. On the treatment of negative intensity observations
20. Protein synthesis
21. Fusidic acid-resistant mutants define three regions in elongation factor G of Salmonella typhimurium
22. The dynamic structure of EF-G studied by fusidic acid resistance and internal revertants
23. Improved methods for building protein models in electron density maps and the location of errors in these models
24. Disassembly of the post-termination complex and reduction of translational error by ribosome recycling factor (RRF)-A possible new target for antibacterial agents
25. Novel roles for classical factors at the interface between translation termination and initiation
26. The role of guanosine-5'-triphosphate in polypeptide elongation
27. The crystal structure of elongation factor EF-Tu from Thermus aquaticus in the GTP conformation
28. Structural aspects of protein synthesis
29. The states, conformational dynamics and fusidic acid resistant mutants of EF-G
30. Crystal structure of the ribosomal protein S6 from Thermus thermophilus
31. Fusidic acid resistant EF-G perturbs the accumulation of ppGpp
32. An intact conformation at the tip of elongation factor G domain IV is functionally important
33. Interaction of elongation factors EF-G and EF-Tu with a conserved loop in 23 S RNA
34. The ribosome-in-pieces: Binding of elongation factor EF-G to oligoribonucleotides that mimic the sarcin/ricin and thiostrepton domains of 23 S ribosomal RNA
35. Crystal structure of the RNA-binding domain of the U1 small nuclear ribonucleoprotein A
36. AMoRe: An automated package for molecular replacement
37. Crystal structure of the ternary complex of Phe-tRNAPhe, EF-Tu, and a GTP analog
38. Processing of X-ray diffraction data collected in oscillation mode
39. Crystal structure at 1.92 Å resolution of the RNA-binding domain of the U1A spliceosomal protein complexed with an RNA hairpin
40. Helix unwinding in the effector region of elongation factor EF-Tu-GDP
41. Ribosomal protein structures: Insights into the architecture, machinery and evolution of the ribosome
42. Crystal structure of Thermotoga maritima ribosome recycling factor: A tRNA mimic
43. Visualization of elongation factor Tu on the Escherichia coli ribosome
44. Large-scale movement of elongation factor G and extensive conformational change of the ribosome during translocation
45. The crystal structure of ribosomal protein L22 from Thermus thermophilus: Insights into the mechanism of erythromycin resistance
46. Some characteristics of and structural requirements for the interaction of 24,25-dihydrofusidic acid with ribosome elongation factor G complexes
47. Mapping the position of translational elongation factor EF-G in the ribosome by directed hydroxyl radical probing
48. Mechanism of the inhibition of protein synthesis by kirromycin. Role of elongation factor Tu and ribosomes
49. Nucleotide sequence of the Thermus thermophilus HB8 gene coding for elongation factor G