Baculoviral transfer vectors for expression of flag® fusion proteins in insect cells

BioTechniques

Volumn 23, Issue 4, 1997, Pages 622-627

  Koh, Sang Seok ^a   Hengartner, Christoph J ^a   Young, Richard A ^a  

^a WHITEHEAD INSTITUTE FOR BIOMEDICAL RESEARCH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

HYBRID PROTEIN; VIRUS VECTOR;

ARTICLE; BACULOVIRUS; EPITOPE MAPPING; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PROMOTER REGION; PROTEIN SYNTHESIS REGULATION; PROTEIN TARGETING; TRANSCRIPTION INITIATION;

EID: 0002274778     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/97234bm14     Document Type: Article

References (16)

1. Alnemri, E.S., T. Fernandes-Alnemri and G. Litwack. 1995. Cloning and expression of four novel isoforms of human interleukin-1β converting enzyme with different apoptotic activities. J. Biol. Chem. 270:4312-4317.
2. Baas, P.W., T.P. Pienkowski and K.S. Kosik. 1991. Processes induced by tau expression in Sf9 cells have an axon-like microtubule organization. J. Cell Biol. 115:1333-1344.
3. Brizzard, B.L., R.G. Chubet and D.L. Vizard. 1994. Immunoaffinity purification of FLAG® epitope-tagged bacterial alkaline phosphatase using a novel monoclonal antibody and peptide elution. BioTechniques 16:730-735.
4. Chubet, R.G. and B.L. Brizzard. 1996. Vectors for expression and secretion of FLAG epitope-tagged proteins in mammalian cells. BioTechniques 20:136-141.
5. Hengartner, C.J., C.M. Thompson, J. Zhang, D.M. Chao, S.-M. Liao, A.J. Koleske, S. Okamura and R.A. Young. 1995 Association of an activator with an RNA polymerase II holoenzyme. Genes Dev. 9:897-910.
6. Hopp, T.P., K.S. Prickett, V.L. Price, R.T. Libby, C.J. March, D.P. Cerretti, D.L. Urdal and P.J. Conlon. 1988. A short polypeptide marker sequence useful for recombinant protein identification and purification. Bio/ Technology 6:1204-1210.
7. Jarvis, D.L. and E.E. Finn. 1996. Modifying the insect cell N-glycosylation pathway with immediate early baculovirus expression vectors. Nat. Biotechnol. 14:1288-1292.
8. Knaus, R., R. Pollock and L. Guarente. 1996. Yeast SUB1 is a suppressor of TFIIB mutations and has homology to the human co-activator PC4. EMBOJ. 15:1933-1940.
9. Kozak, M. 1987. An analysis of 5′-noncoding sequences from 699 vertebrate messenger RNAs. Nucleic Acids Res. 15:8125-8148.
10. Kozak, M. 1989. The scanning model for translation: an update. J. Cell Biol. 108:229-241.
11. Li, S., K.S. Song, S.S. Koh, A. Kikuchi and M.P. Lisanti. 1996. Baculovirus-based expression of mammalian caveolin in Sf21 insect cells: a model system for the biochemical and morphological study of caveolae biogenesis. J. Biol. Chem. 271:28647-28654.
12. Miller, L.K. 1993. Baculoviruses: high-level expression in insect cells. Curr. Opin. Genet. Dev. 3:97-101.
13. O'Reilly, D.R., L.K. Miller and V.A. Luckow. 1992. Baculovirus Expression Vectors. A Laboratory Manual. W.H. Freeman, New York.
14. Palhan, V.B., S. Sumathy and K.P. Gopinathan. 1995. Baculovirus mediated high-level expression of luciferase in silkworm cells and larvae. BioTechniques 19:97-104.
15. Thompson, C.M., A.J. Koleske, D.M. Chao and R.A. Young. 1993. A multisubunit complex associated with the RNA polymerase II CTD and TATA-binding protein in yeast. Cell 73:1361-1375.
16. Williams, F.E. and R.J. Trumbly. 1990. Characterization of TUP1, a mediator of glucose repression in Saccharomyces cerevisiae. Mol. Cell. Biol. 10:6500-6511.