Molecular analysis of DHFR and DHPS genes in P. falciparum clinical isolates from the Haut-Ogooué region in Gabon

Acta Tropica

Volumn 78, Issue 3, 2001, Pages 231-240

  Mawili Mboumba, Denise Patricia ^a   Ekala, Marie Thérèse ^a   Lekoulou, Faustin ^a   Ntoumi, Francine ^a,b  

^a CENTRE INTERNATIONAL DE RECHERCHES MÉDICALES DE FRANCEVILLE   (Gabon)

^b UNIVERSITY OF TÜBINGEN   (Germany)

Author keywords

DHFR; DHPS; Gabon; Plasmodium falciparum

Indexed keywords

AMINO ACID; ASPARAGINE; DIHYDROFOLATE REDUCTASE; DIHYDROPTEROATE SYNTHASE; FANSIDAR; FOLIC ACID ANTAGONIST; SERINE; THREONINE;

DRUG RESISTANCE; GENETIC ANALYSIS; MOLECULAR ANALYSIS;

ARTICLE; BLOOD SAMPLING; CODON; COMPARATIVE STUDY; DNA SEQUENCE; ENZYME ACTIVITY; GABON; GENETIC ANALYSIS; GENETIC CODE; HUMAN; MOLECULAR GENETICS; PLASMODIUM FALCIPARUM; POINT MUTATION; POLYMERASE CHAIN REACTION; PREVALENCE;

ADOLESCENT; ADULT; AMINO ACID SEQUENCE; ANIMALS; CHILD; CHILD, PRESCHOOL; DIHYDROPTEROATE SYNTHASE; DNA, PROTOZOAN; DRUG RESISTANCE, MULTIPLE; FEMALE; GABON; HUMANS; INFANT; MALARIA, FALCIPARUM; MALE; MIDDLE AGED; MOLECULAR SEQUENCE DATA; PLASMODIUM FALCIPARUM; POINT MUTATION; POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS, DNA; SEQUENCE HOMOLOGY, AMINO ACID; TETRAHYDROFOLATE DEHYDROGENASE;

GABON;

PLASMODIUM FALCIPARUM;

EID: 0035970472     PISSN: 0001706X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0001-706X(01)00084-5     Document Type: Article

References (31)

1. Molecular epidemiology of malaria in Yaounde, Cameroon II. Baseline frequency of point mutations in the dihydropteroate synthase gene of Plasmodium falciparum
2. Molecular epidemiology of malaria in Yaounde, Cameroon I. Analysis of point mutations in the dihydrofolate reductase-thymidilate synthase gene of Plasmodium falciparum
3. In vitro activity of pyrimethamine, cycloguanil and other antimalarial drugs against African isolates and clones of Plasmodium falciparum
4. Point mutations in the dihydrofolate reductase-thymidilate synthase gene and pyrimethamine and cycloguanil resistance in Plasmodium falciparum
5. Sequence variation of the hydroxymethyldihydropterin pyrophosphokinase: Dihydropteroate synthase gene in lines of the human malaria parasite, Plasmodium falciparum, with differing resistance sulfadoxine
6. Amino acid changes linked to pyrimethamine resistance in the dihydrofolate reductase-thymidilate synthase gene of Plasmodium falciparum
7. Direct evidence that asparagine at position 108 of the Plasmodium falciparum dihydrofolate reductase is involved in resistance to antifolate drugs in Tanzania
8. Sulfadoxine-pyrimethamine for treatment of Plasmodium falciparum malaria in Gabonese children
9. Malaria Transmission in a region of savannah -forest mosaic, Haut-Ogooué, Gabon
10. Amino acids in the dihydrofolate reductase-thymidilate synthase gene of Plasmodium falciparum involved in cycloguanil resistance differ from those involved in pyrimethamine resistance
11. Frequency of the Asn-108 and Thr-108 point mutations in the dihydrofolate reductase gene in Plasmodium falciparum from South West COL
12. Molecular assays for surveillance of antifolate-resistant malaria
13. Low-dose treatment with sulfadoxine -pyrimethamine combinations selects for drug-resistant Plasmodium falciparum strains
14. Age-dependent carriage of multiple Plasmodium falciparum merozoite surface antigen-2 alleles in asymptomatic malaria infections
15. Plasmodium falciparum: Sickle-cell trait is associated with higher prevalence of multiple infections in gabonese children with asymptomatic infections
16. Proguanil resistance in Plasmodium falciparum African isolates: Assessment by mutation- specific polymerase chain reaction and in vitro susceptibility testing
17. Evidence that a point mutation in dihydrofolate reductase-thymidilate synthase confers resistance to pyrimethamine in falciparum malaria
18. Molecular basis of differential resistance to cycloguanil and pyrimethamine in Plasmodium falciparum
19. Prevalence of the dihydrofolate reductase Asn-108 mutation as the basis for pyrimethamine-resistant falciparum malaria in the Brazilian Amazon
20. Follow-up of the susceptibility of Plasmodium falciparum to antimalarials in Gabon
21. Community pyrimethamine-sulfadoxine use and prevalence of resistant Plasmodium falciparum genotypes in Mali: A model for deterring resistance
22. Mutations in Plasmodium falciparum dihydrofolate reductase and dihydropteroate synthase and epidemiologic patterns of pyrimethamine-sulfadoxine use and resistance
23. Molecular analysis of recrudescent parasites in a Plasmodium falciparum drug efficacy trial in Gabon
24. Point mutations in the dihydrofolate reductase and dihydropteroate synthetase genes and in vitro susceptibility to pyrimethamine and cycloguanil of Plasmodium falciparum isolates from Papua New Guinea
25. The dihydrofolate reductase domain of Plasmodium falciparum thymidylate synthase-dihydrofolate reductase
26. Impact of Chloroquine resistance on malaria mortality
27. Primary structure and expression of the dihydropteroate synthetase gene of Plasmodium falciparum
28. A mutation-specific PCR system to detect sequence variation in the dihydropteroate synthetase gene of Plasmodium falciparum
29. Resistance to antifolates in Plasmodium falciparum monitored by sequence analysis of dihydropteroate synthetase and dihydrofolate reductase alleles in a large number of field samples of diverse origins
30. Sulfadoxine resistance in the human malaria parasite Plasmodium falciparum is determined by mutations in dihydropteroate synthetase and an additional factor associated with folate utilization
31. Pyrimethamine-sulfadoxine efficacy and selection for mutations in Plasmodium falciparum dihydrofolate reductase and dihydropteroate synthase in Mali