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note
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The organization of the bilayer at the edges is not known. Presumably, the top and the bottom leaflets connect in some manner to keep the hydrophobic interior away from water. The result is an unfavorable line energy that should cause an isolated square such as the ones in Figure 2 to become circular. However, we have not observed any reorganization for any of the shapes printed or blotted, indicating that large-scale reorganization is prevented, probably by entanglement in pinning defects on the surface.
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0011326243
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note
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We note that we have also looked at vesicle fusion on unoxidized PDMS. From fluorimetry and FRAP measurements, there are approximately 40% the number of lipids on these surfaces as on glass and there is long-range diffusion, with diffusion coefficients slightly slower than those on glass. These results indicate that it is possible to form a mobile monolayer on hydrophobic PDMS.
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