Methyl-specific DNA binding by McrBC, a modification-dependent restriction enzyme

Journal of Molecular Biology

Volumn 298, Issue 4, 2000, Pages 611-622

  Stewart, Fiona J ^a   Panne, Daniel ^b   Bickle, Thomas A ^b   Raleigh, Elisabeth A ^a  

^a NEW ENGLAND BIOLABS   (United States)

^b UNIVERSITY OF BASEL   (Switzerland)

Author keywords

Footprint; Gel shift; GTP dependent; Nucleotide dependent; RglB

Indexed keywords

RESTRICTION ENDONUCLEASE;

ARTICLE; BINDING SITE; CHEMICAL MODIFICATION; CONFORMATIONAL TRANSITION; DNA FOOTPRINTING; ESCHERICHIA COLI K 12; NONHUMAN; PRIORITY JOURNAL; PROTEIN DNA BINDING; SEQUENCE ANALYSIS; STRUCTURE ANALYSIS;

ESCHERICHIA; ESCHERICHIA COLI; ESCHERICHIA COLI K12;

EID: 0034640130     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.2000.3697     Document Type: Article

References (30)

1. Unwinding of closed circular DNA by the Escherichia coli RuvA and RuvB recombination/repair proteins
2. Barriers to recombination: Restriction
3. Evidence of participation of McrB(S) in McrBC restriction in Escherichia coli K-12
4. Biology of DNA restriction
5. Gel retardation
6. Restriction and modification
7. Genetic and sequence organization of the mcrBC locus of Escherichia coli K-12
8. The recognition of methylated DNA by the GTP-dependent restriction endonuclease McrBC resides in the N-terminal domain of McrB
9. Branch migration during homologous recombination: Assembly of a RuvAB-Holliday junction complex in vitro
10. Detecting cooperative protein-DNA interactions and DNA loop formation by foot-printing
11. Detection of localized DNA flexibility
12. A novel activity in Escherichia coli K-12 that directs restriction of DNA modified at CG dinucleotides
13. Restriction enzymes in cells, not eppendorfs
14. Lac repressor forms loops with linear DNA carrying two suitably spaced lac operators
15. Characterization of the mcrBC region of E. coli K-12 wild-type and mutant strains
16. McrB: A prokaryotic protein specifically recognizing DNA containing modified cytosine residues
17. Use of gel retardation to analyze protein-nucleic acid interactions
18. Type III restriction endonucleases translocate DNA in a reaction driven by recognition site-specific ATP hydrolysis
19. McrBs, a modulator peptide for McrBC activity
20. The McrBC endonuclease translocates DNA in a reaction dependent on GTP hydrolysis
21. Characterization of the interaction between the restriction endonuclease McrBC from E. coli and its cofactor GTP
22. Organization and function of the mcrBC genes of E. coli K-12
23. DNA modification: Glucosylation
24. Nucleotide sequence of the McrB region of Escherichia coli K-12 and evidence for two independent translational initiation sites at the mcrB locus
25. Dependence of McrBC cleavage on distance between recognition elements
26. Use of T7 RNA polymerase to direct expression of cloned genes
27. McrBC: A multisubunit GTP-dependent restriction endonuclease
28. Phosphatases and Kinases (unit 3.1)
29. Processing of recombination intermediates by the RuvABC proteins