Cationized human serum albumin as a non-viral vector system for gene delivery? Characterization of complex formation with plasmid DNA and transfection efficiency

International Journal of Pharmaceutics

Volumn 225, Issue 1-2, 2001, Pages 97-111

  Fischer, Dagmar ^a   Bieber, Thorsten ^a   Brüsselbach, Sabine ^a   Elsässer, Hans Peter ^a   Kissel, Thomas ^a  

^a PHILIPPS UNIVERSITY MARBURG   (Germany)

Author keywords

Absorptive endocytosis; Cationized human serum albumin; Confocal laser scanning microscopy; Gene transfer

Indexed keywords

1,6 HEXANEDIAMINE; CARBOXYL GROUP; CHLOROQUINE; DNA; HUMAN SERUM ALBUMIN; PLASMID DNA; SODIUM CHLORIDE;

AGAR GEL ELECTROPHORESIS; ANIMAL CELL; ARTICLE; CELL AGGREGATION; CELL STRAIN 3T3; CONFOCAL LASER MICROSCOPY; CONTROLLED STUDY; COVALENT BOND; DNA DRUG COMPLEX; DNA TRANSFECTION; ENDOCYTOSIS; GENE EXPRESSION; GENE TARGETING; GENE TRANSFER; INCUBATION TIME; IONIC STRENGTH; IONIZATION; ISOELECTRIC POINT; MOUSE; NONHUMAN; PH; PRIORITY JOURNAL; PROTEIN ASSEMBLY; REPORTER GENE; SPECTROSCOPY; TEMPERATURE;

DNA; ELECTROPHORESIS, AGAR GEL; GENE TRANSFER TECHNIQUES; HUMANS; LAC OPERON; MICROSCOPY, CONFOCAL; PLASMIDS; SERUM ALBUMIN;

EID: 0035964457     PISSN: 03785173     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-5173(01)00765-7     Document Type: Article

References (40)

1. Cationization of bovine serum albumin alters its conformation as well as its charge
2. Gene transfer with synthetic cationic amphiphiles: Prospects for gene therapy
3. Effect of size and serum proteins on transfection efficiency of poly((2-dimethylamino)ethyl methacrylate)-plasmid nanoparticles
4. In vitro cytotoxicity of macromolecules in different cell culture systems
5. Gene vaccination with naked plasmid DNA: Mechanism of CTL priming
6. Transferrin-polycation-mediated introduction of DNA into human leukemic cells: Stimulation by agents that effect the survival of transfected DNA or modulate transferrin receptor levels
7. Enhanced gene delivery and mechanism studies with a novel series of cationic lipid formulations
8. Comparative in vitro cytotoxicity studies of polycations for gene therapy
9. Cationic low molecular weight polymers for gene transfer: Cytotoxicity and transfection efficiency
10. A novel nonviral vector for DNA delivery based on low molecular weight, branched polyethylenimine: Effect of molecular weight on transfection efficiency and cytotoxicity
11. Method for the quantitative modification and estimation of carboxylic acid groups in proteins
12. Evidence for targeted gene transfer by receptor-mediated endocytosis. Stable expression following insulin-directed entry of NEO into HepG2 cells
13. Absorptive-mediated endocytosis of cationized albumin and a β-endorphin-cationized albumin chimeric peptide by isolated brain capillaries
14. Polymer systems for cardiovascular drug delivery
15. Nonviral gene therapy: The promise of genes as pharmaceutical products
16. Pharmaceutical approach to somatic gene therapy
17. Specific gene transfer mediated by lactosylated poly-L-lysine into hepatoma cells
18. Targeted vectors for gene therapy
19. Characterization and biological implications of membrane lectins in tumor, lymphoid and myeloid cells
20. Albumin nanospheres as carriers for passive drug targeting: An optimized manufacturing technique
21. The basic science of gene therapy
22. Polyvinyl derivatives as novel interactive polymers for controlled gene delivery to muscle
23. New delivery system for plasmid DNA in vivo using atelocollagen as a carrier material: The Minipellet
24. Absence of albumin receptor on brain capillaries in vivo or in vitro
25. Evaluation of cationized rat albumin as a potential blood-brain barrier drug transport vector
26. β-Endorphin chimeric peptides: Transport through the blood-brain barrier in vivo and cleavage of disulfide linkage by brain
27. Serum albumin
28. A poly(L-lysine)/anti-thrombomodulin conjugate for targeted gene delivery to endothelial cells
29. Surface-modified biodegradable albumin nano- and microspheres. I. Preparation and characterization
30. Humoral and cellular immunities elicited by DNA vaccines: Application to the human immunodeficiency virus and influenza
31. The influence of polymer structure on the interactions of cationic polymers with DNA and morphology of the resulting complexes
32. Texas Red, a hydrophilic, red-emitting fluorophore for use with fluorescein in dual parameter flow microfluorometric and fluorescence microscopic study
33. Controllable gene therapy: Pharmaceutics of non-viral gene delivery systems
34. Condensation of plasmid DNA with polylysine improves liposome-mediated gene transfer into established and primary muscle cells
35. Ultrastructural study of transcellular transport of native and cationized albumin in cultured sheep brain microvascular endothelium
36. Influenza virus hemagglutinin HA-2 N-terminal fusogenic peptides augment gene transfer by transferrin-polylysine-DNA complexes: Toward a synthetic virus-like gene-transfer vehicle
37. Coupling of adenovirus to transferrin-polylysine/DNA complexes greatly enhances receptor-mediated gene delivery and expression of transfected genes
38. Characterization of vectors for gene therapy formed by self-assembly of DNA with synthetic block co-polymers
39. Direct gene transfer into mouse muscle in vivo