Monitoring receptor oligomerization using time-resolved fluorescence resonance energy transfer and bioluminescence resonance energy transfer. The human δ-opioid receptor displays constitutive oligomerization at the cell surface, which is not regulated by receptor occupancy

Journal of Biological Chemistry

Volumn 276, Issue 17, 2001, Pages 14092-14099

  McVey, Mary ^a   Ramsay, Douglas ^a   Kellett, Elaine ^a   Rees, Stephen ^b   Wilson, Shelagh ^b   Pope, Andrew J ^b   Milligan, Graeme ^a,c  

^a UNIVERSITY OF GLASGOW   (United Kingdom)

^b GLAXOSMITHKLINE   (United Kingdom)

^c UNIVERSITY OF GLASGOW   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

BIOLUMINESCENCE; ENERGY TRANSFER; FLUORESCENCE; IMMUNOLOGY; OLIGOMERS; PROTEINS;

INVERSE AGONIST LIGANDS;

CELLS;

ADENYLATE CYCLASE; BETA 2 ADRENERGIC RECEPTOR; DELTA OPIATE RECEPTOR; DIHYDROALPRENOLOL; DIPRENORPHINE; DNA; ENKEPHALIN[2 DEXTRO ALANINE 5 DEXTRO LEUCINE]; FORSKOLIN; G PROTEIN COUPLED RECEPTOR; LIGAND; LUCIFERASE; N,N DIALLYLTYROSYL 2 METHYLALANYL 2 METHYLALANYLPHENYLALANYLLEUCINE; OLIGOMER; OPIATE; TRITIUM; DRUG DERIVATIVE; ENKEPHALIN; EPITOPE; LEUCINE ENKEPHALIN; N,N DIALLYL TYROSYL ALPHA AMINOISOBUTYRIC ACID PHENYLALANYL LEUCINE; N,N-DIALLYL-TYROSYL-ALPHA-AMINOISOBUTYRIC ACID-PHENYLALANYL-LEUCINE; NARCOTIC ANTAGONIST;

ARTICLE; BIOLUMINESCENCE; DRUG RECEPTOR BINDING; ENERGY TRANSFER; ENZYME ACTIVITY; FLUORESCENCE; FLUORESCENCE RESONANCE ENERGY TRANSFER; HUMAN; IMMUNOPRECIPITATION; ISOTOPE LABELING; LIGAND BINDING; NUCLEOTIDE SEQUENCE; OLIGOMERIZATION; PLASMID; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RECEPTOR OCCUPANCY; CELL LINE; CELL MEMBRANE; CHEMISTRY; GENETIC TRANSFECTION; IMMUNOBLOTTING; LUMINESCENCE; METABOLISM; METHODOLOGY; PROTEIN BINDING; SERODIAGNOSIS; SIGNAL TRANSDUCTION; SPECTROFLUOROMETRY; TIME; WESTERN BLOTTING;

HOMO;

BLOTTING, WESTERN; CELL LINE; CELL MEMBRANE; ENKEPHALIN, LEUCINE; ENKEPHALINS; EPITOPES; HUMANS; IMMUNOBLOTTING; LIGANDS; LUMINESCENT MEASUREMENTS; NARCOTIC ANTAGONISTS; PLASMIDS; PRECIPITIN TESTS; PROTEIN BINDING; RECEPTORS, ADRENERGIC, BETA-2; RECEPTORS, OPIOID, DELTA; SIGNAL TRANSDUCTION; SPECTROMETRY, FLUORESCENCE; TIME FACTORS; TRANSFECTION;

EID: 0035958023     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.M008902200     Document Type: Article

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