Mammalian (cytosine-5) methyltransferases cause genomic DNA methylation and lethality in Drosophila

Nature Genetics

Volumn 23, Issue 3, 1999, Pages 363-366

  Lyko, Frank ^a   Ramsahoye, Bernard H ^b   Kashevsky, Helena ^a   Tudor, Matthew ^a   Mastrangelo, Mary Ann ^a   Orr Weaver, Terry L ^a,c   Jaenisch, Rudolf ^a,c  

^a WHITEHEAD INSTITUTE FOR BIOMEDICAL RESEARCH   (United States)

^b CARDIFF UNIVERSITY   (United Kingdom)

^c MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

METHYLTRANSFERASE;

ARTICLE; DNA METHYLATION; DROSOPHILA MELANOGASTER; GENE CONTROL; GENOME; LETHALITY; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; STEM CELL; TRANSGENE;

ANIMALS; ANIMALS, GENETICALLY MODIFIED; CPG ISLANDS; DNA (CYTOSINE-5-)-METHYLTRANSFERASE; DNA METHYLATION; DROSOPHILA MELANOGASTER; GENES, LETHAL; GENOME; LARVA; MICE; PHENOTYPE; PUPA; TRANSGENES;

ARACHNIDA; DROSOPHILA MELANOGASTER; HEXAPODA; MAMMALIA; MELANOGASTER;

EID: 0032697888     PISSN: 10614036     EISSN: None     Source Type: Journal    
DOI: 10.1038/15551     Document Type: Article

References (31)

1. Li, E., Bestor, T.H. & Jaenisch, R. Targeted mutation of the DNA methyltransferase gene results in embryonic lethality. Cell 69, 915-926 (1992).
2. Li, E., Beard, C. & Jaenisch, R. Role for DNA methylation in genomic imprinting. Nature 366, 362-365 (1993).
3. Panning, B. & Jaenisch, R. DNA hypomethylation can activate Xist expression and silence X-linked genes. Genes Dev. 10, 1991-2002 (1996).
4. Chen, R.Z., Pettersson, U., Beard, C., Jackson-Grusby, L. & Jaenisch, R. DNA hypomethylation leads to elevated mutation rates. Nature 395, 89-93 (1998).
5. Walsh, C.P., Chaillet, J.R. & Bestor, T.H. Transcription of IAP endogenous retroviruses is constrained by cytosine methylation. Nature Genet. 20, 116-117 (1998).
6. Jaenisch, R. DNA methylation and imprinting: why bother? Trends Genet. 13, 323-329 (1997).
7. Gruenbaum, Y., Cedar, H. & Razin, A. Substrate and sequence specificity of a eukaryotic DNA methylase. Nature 295, 620-622 (1982).
8. Bestor, T.H. & Ingram, V.M. Two DNA methyltransferases from murine erythroleukemia cells: purification, sequence specificity, and mode of interaction with DNA. Proc. Natl Acad. Sci. USA 80, 5559-5563 (1983).
9. Yoder, J.A., Soman, N.S., Verdine, G.L. & Bestor, T.H. DNA (cytosine-5)-methyltransferases in mouse cells and tissues. Studies with a mechanism-based probe. J. Mol. Biol. 270, 385-395 (1997).
10. Lei, H. et al. De novo DNA cytosine methyltransferase activities in mouse embryonic stem cells. Development 122, 3195-3205 (1996).
11. Okano, M., Xie, S. & Li, E. Cloning and characterization of a family of novel mammalian DNA (cytosine-5) methyltransferases. Nature Genet. 19, 219-220 (1998).
12. Urieli-Shoval, S., Gruenbaum, Y., Sedat, J. & Razin, A. The absence of detectable methylated bases in Drosophila melanogaster DNA. FEBS Lett. 146, 148-152 (1982).
13. Patel, C.V. & Gopinathan, K.P. Determination of trace amounts of 5-methylcytosine in DNA by reverse-phase high-performance liquid chromatography. Anal. Biochem. 164, 164-169 (1987).
14. Brand, A.H., Manoukian, A.S. & Perrimon, N. Ectopic expression in Drosophila. Methods Cell Biol. 44, 635-654 (1994).
15. Sutherland, E., Coe, L. & Raleigh, E.A. McrBC: a multisubunit GTP-dependent restriction endonuclease. J. Mol. Biol. 225, 327-348 (1992).
16. Danilevskaya, O., Slot, F., Pavlova, M. & Pardue, M.L Structure of the Drosophila HeT-A transposon: a retrotransposon-like element forming telomeres. Chromosoma 103, 215-224 (1994).
17. Bestor, T.H. Activation of mammalian DNA methyltransferase by cleavage of a Zn binding regulatory domain. EMBO J. 11, 2611-2617 (1992).
18. Holliday, R. & Pugh, J.E. DNA modification mechanisms and gene activity during development. Science 187, 226-232 (1975).
19. Riggs, A.D. X inactivation, differentiation, and DNA methylation. Cytogenet. Cell Genet. 14, 9-25 (1975).
20. Tucker, K.L. et al. Germ-line passage is required for establishment of methylation and expression patterns of imprinted but not of nonimprinted genes. Genes Dev. 10, 1008-1020 (1996).
21. Wines, D.R., Talbert, P.B., Clark, D.V. & Henikoff, S. Introduction of a DNA methyltransferase into Drosophila to probe chromatin structure in vivo. Chromosomi 104, 332-340 (1996).
22. Becker, P.B., Ruppert, S. & Schütz, G. Genomic footprinting reveals cell type-specific DNA binding of ubiquitous factors. Cell 51, 435-443 (1987).
23. Iguchi-Ariga, S.M. & Schaffner, W. CpG methylation of the cAMP-responsive enhancer/promoter sequence TGACGTCA abolishes specific factor binding as well as transcriptional activation. Genes Dev. 3, 612-619 (1989).
24. Ng, H.H. & Bird, A. DNA methylation and chromatin modification. Curr. Opin. Genet. Dev. 9, 158-163 (1999).
25. Kudo, S. Methyl-CpG-binding protein MeCP2 represses Sp-1 activated transcription of the human leukosialin gene when the promoter is methylated. Mol. Cell. Biol. 18, 5492-5499 (1998).
26. Wade, P.A. et al. Mi-2 complex couples DNA methylation to chromatin remodelling and histone deacetylation. Nature Genet. 23, 62-66 (1999).
27. Spradling, A.L. & Rubin, G.M. Transformation of cloned P elements into Drosophila germ line chromosomes. Science 218, 341-347 (1982).
28. Zink, D. & Pare, R. Drosophila Polycomb-group regulated chromatin inhibits the accessibility of a trans-activator to its target DNA. EMBO J. 14, 5660-5671 (1995).
29. Ashburner, M. Drosophila: A Laboratory Manual (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, 1989).
30. Kumar, S., Cheng, X., Pflugrath, J.W. & Roberts, R.J. Purification, crystallization, and preliminary X-ray diffraction analysis of an M.Hhal-AdoMet complex. Biochemistry 31, 8648-8653 (1992).
31. Gruenbaum, Y., Stein, R., Cedar, H. & Razin, A. Methylation of CpG sequences in eukaryotic DNA. FEBS Lett. 124, 67-71 (1981).