Agonist-stimulated [35S]GTPγS autoradiography: Optimization for high sensitivity

European Journal of Pharmacology

Volumn 422, Issue 1-3, 2001, Pages 1-13

  Happe, H Kevin ^a   Bylund, David B ^a   Murrin, L Charles ^a  

^a UNIVERSITY OF NEBRASKA MEDICAL CENTER   (United States)

Author keywords

2 adrenergic receptor; Opiate receptor; 5 HT1A receptor; G protein coupled receptor; Signal transduction

Indexed keywords

4 (2 HYDROXYETHYL) 1 PIPERAZINEETHANESULFONIC ACID; 5 CARBAMOYLTRYPTAMINE; ADRENALIN; ALPHA 2 ADRENERGIC RECEPTOR; DITHIOTHREITOL; GLYCYLGLYCINE; GUANINE NUCLEOTIDE BINDING PROTEIN; GUANOSINE 5' O (3 THIOTRIPHOSPHATE); GUANOSINE DIPHOSPHATE; METHADONE; MU OPIATE RECEPTOR; SEROTONIN 1A RECEPTOR; SULFUR 35; TROMETAMOL; 1,3-DIPROPYL-8-CYCLOPENTYLXANTHINE; 5-CARBOXAMIDOTRYPTAMINE; 8 CYCLOPENTYL 1,3 DIPROPYLXANTHINE; ADENOSINE RECEPTOR; ADRENERGIC RECEPTOR STIMULATING AGENT; BUFFER; DIAGNOSTIC AGENT; DRUG DERIVATIVE; MAGNESIUM; SEROTONIN; SEROTONIN 1 RECEPTOR; SEROTONIN AGONIST; SEROTONIN RECEPTOR; SODIUM CHLORIDE; SULFUR; XANTHINE DERIVATIVE;

ANIMAL TISSUE; ARTICLE; ASSAY; AUTORADIOGRAPHY; BRAIN; CONTROLLED STUDY; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; RAT; RECEPTOR BINDING; RECEPTOR SENSITIVITY; SIGNAL NOISE RATIO; TISSUE SECTION; ANIMAL; BINDING COMPETITION; DOSE RESPONSE; DRUG ANTAGONISM; DRUG EFFECT; DRUG POTENTIATION; METABOLISM; SENSITIVITY AND SPECIFICITY; SPRAGUE DAWLEY RAT; TIME;

ADRENERGIC AGONISTS; ANIMALS; AUTORADIOGRAPHY; BINDING, COMPETITIVE; BRAIN; BUFFERS; DITHIOTHREITOL; DOSE-RESPONSE RELATIONSHIP, DRUG; EPINEPHRINE; GUANOSINE 5'-O-(3-THIOTRIPHOSPHATE); GUANOSINE DIPHOSPHATE; MAGNESIUM; METHADONE; RATS; RATS, SPRAGUE-DAWLEY; RECEPTORS, ADRENERGIC, ALPHA-2; RECEPTORS, PURINERGIC P1; RECEPTORS, SEROTONIN; RECEPTORS, SEROTONIN, 5-HT1; SENSITIVITY AND SPECIFICITY; SEROTONIN; SEROTONIN AGONISTS; SODIUM CHLORIDE; SULFUR RADIOISOTOPES; TIME FACTORS; XANTHINES;

EID: 0035933274     PISSN: 00142999     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0014-2999(01)01043-3     Document Type: Article

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