Effect of water activity and immobilization on fatty acid selectivity for esterification reactions mediated by lipases

Biotechnology and Bioengineering

Volumn 75, Issue 2, 2001, Pages 219-227

  Lee, Chen Hsien ^a   Parkin, Kirk L ^a  

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

Author keywords

Esterification; Fatty acid; Immobilization; Lipase; Selectivity; Water activity

Indexed keywords

CELL IMMOBILIZATION; ENZYMES; ESTERIFICATION; ESTERS; LIPIDS; WATER;

COSUBSTRATES;

FATTY ACIDS;

FATTY ACID; TRIACYLGLYCEROL LIPASE;

ARTICLE; BURKHOLDERIA CEPACIA; CANDIDA ANTARCTICA; CANDIDA RUGOSA; ENZYME ACTIVITY; ENZYME IMMOBILIZATION; ENZYME SPECIFICITY; ESTERIFICATION; NONHUMAN; RHIZOMUCOR;

ANION EXCHANGE RESINS; BACTERIA; BINDING SITES; BURKHOLDERIA CEPACIA; CANDIDA; ENZYME ACTIVATION; ESTERIFICATION; FATTY ACIDS; FUNGI; IMMOBILIZATION; KINETICS; LIPASE; PROPYLENE GLYCOLS; RHIZOMUCOR; SUBSTRATE SPECIFICITY; TIME FACTORS; WATER;

EID: 0035922873     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.10009     Document Type: Article

References (48)

1. On the importance of the support material for enzymatic synthesis in organic media. Support effects at controlled water activity
2. Enzymatic catalysis and dynamics in low-water environment
3. Hydrolysis of edible oils by lipases immobilized on hydrophobic supports: Effects of internal support structure
4. Thermal stabilization of immobilized lipase B from Candida antarctica on different supports: Effect of water activity on enzymatic activity in organic media
5. Biocatalyst behaviour in low-water systems
6. Lipases in organic solvents; the fatty acid chain length profile
7. Activity and stability of lipase in the solid-phase glycerolysis of triolein
8. Water activity does not influence the enantioselectivity of lipase PS and lipoprotein-lipase in organic solvents
9. M of lipase catalyzed transesterification in organic media
10. Comparative selectivities of immobilized lipases from Pseudomonas cepacia and Candida antarctica (fraction B) for esterification reactions with glycerol and glycerol analogues in organic media
11. Influence of water activity on the synthesis of triolein catalyzed by immobilized Mucor miehei lipase
12. Effect of the immobilization support on the hydrolytic activity of a cutinase from Fusarium solani pisi
13. Enzymes as biocatalyst in the modification of natural lipids
14. Salt hydrates for water activity control with biocatalysts in organic media
15. Thermodynamic predictions for biocatalysis in nonconventional media: Theory, test, and recommendations for experimental design and analysis
16. Water activity influences enantioselectivity in a lipase-catalysed reaction by esterification in an organic solvent
17. Selectivity is an important characteristic of lipases (acylglycerol hydrolases)
18. Elucidating structure-mechanism relationships in lipases: Prospects for predicting and engineering catalytic properties
19. Enzyme immobilization
20. The effect of immobilization on the hydrolytic/interesterification activity of lipase from Rhizopus niveus
21. Additive effects of acyl-binding site mutations on the fatty acid selectivity of Rhizopus delemar lipase
22. Why are enzymes less active in organic solvents than in water?
23. Working at controlled water activity in a continuous process: The gas/solid system as a solution
24. Comparative fatty acid selectivity of lipases in esterification reactions with glycerol and diol analogues in organic media
25. Immobilized lipase reactors for modification of fats and oils - A review
26. Substrate specificities of lipases in view of kinetic resolution of unsaturated fatty acids
27. Reaction rates in organic media show similar dependence on water activity with lipase catalyst immobilized on different supports
28. Tailoring lipase specificity by solvent and substrate chemistries
29. Activity of Pseudomonas cepacia lipase in organic media is greatly enhanced after immobilization on a polypropylene support
30. Properties of free and immobilized lipase from Pseudomonas cepacia
31. Selectivity of Celite-immobilized patain (lipid acyl hydrolase from potato (Solanum tuberosum L.) tubers in esterification reactions as influenced by water activity and glycerol analogues as alcohol acceptors
32. Reaction of glycerol with fatty acids in the presence of ion-exchange resins. Preparation of monoglycerides
33. Multicompetitive enzymatic reactions in organic media: A simple test for the determination of lipase fatty acid specificity
34. Water activity control in lipase-catalyzed reaction system
35. On the importance of the support material for bioorganic syntheses. Influence of water partition between solvent, enzyme and solid support in water-poor reaction media
36. Lipases: Interfacial enzymes with attractive applications
37. Optimization of Pseudomonas cepacia lipase preparations for catalysis in organic solvents
38. Pseudomonas cepacia lipase: Esterification reactions in AOT microemulsion systems
39. Effects of water activity on reaction rates and equilibrium positions in enzymatic esterification
40. Lipases from different sources vary widely in dependence of catalytic activity on water activity
41. Reaction rate with suspended lipase catalytic shows similar dependence on water activity in different organic solvents
42. Relationship between water activity and catalytic activity of lipases in organic media. Effects of supports, loading and enzyme preparation
43. The comparative discriminating abilities of lipases in different media and their application in fatty acid enrichment
44. Lipases have similar water activity profile in different reactions
45. Enantioselectivity of lipases: Effects of water activity
46. Enzymic interesterification of fats: The effect of non-lipase material on immobilized enzyme activity
47. Ester synthesis in lipase-catalyzed reactions
48. Selection of salt hydrate pairs for use in water control in enzyme catalysis in organic solvents