Activity of Pseudomonas cepacia lipase in organic media is greatly enhanced after immobilization on a polypropylene support

Applied Microbiology and Biotechnology

Volumn 47, Issue 6, 1997, Pages 630-635

  Pencreac'h, Gaëlle ^a   Baratti, Jacques C ^a  

^a AIX MARSEILLE UNIVERSITY   (France)

Author keywords

[No Author keywords available]

Indexed keywords

ACETIC ACID DERIVATIVE; BACTERIAL ENZYME; ORGANIC COMPOUND; ORGANIC SOLVENT; PALMITIC ACID DERIVATIVE; POLYPROPYLENE; TRIACYLGLYCEROL LIPASE;

ARTICLE; BURKHOLDERIA CEPACIA; CULTURE MEDIUM; ENZYME ACTIVITY; ENZYME IMMOBILIZATION; ENZYME KINETICS; HYDROLYSIS; NONHUMAN;

BURKHOLDERIA CEPACIA; DIFFUSION; ENZYMES, IMMOBILIZED; KINETICS; LIPASE; POLYPROPYLENES;

BACTERIA (MICROORGANISMS); BOVINAE; BURKHOLDERIA; BURKHOLDERIA CEPACIA; FELIS CATUS; PSEUDOMONAS;

EID: 0030787811     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s002530050986     Document Type: Article

References (18)

1. Balcao VM, Paiva AL, Malcata XF (1996) Bioreactors with immobilized lipases: state of the art. Enzyme Microb Technol 18: 392-416
2. Basri M, Ampon K, Wan Yunus WMZ, Razak CNA, Salleh AB (1995) Enzymic synthesis of fatty esters by hydrophobic lipase derivatives immobilized on organic polymer beads. J Am Oil Chem Soc 72: 407-411
3. Chitaba I, Tosa T, Sato T, Mori T (1978) Properties of immobilized enzymes and microbial cells. In: Chibata I (ed) Immobilized enzymes. Halsted Press, New York, pp 108-144
4. Cho SW, Rhee JS (1993) Immobilization of lipase for effective interesterification of fats and oils in organic solvent. Biotechnol Bioeng 41: 204-210
5. Day SH, Legge RL (1995) Immobilization of tyrosinase for use in nonaqueous media: enzyme deactivation phenomena. Biotechnol Tech 9: 471-476
6. Geluk MA, Norde W, Van Kalsbeck HKAI, Van't Riet K (1992) Adsorption of lipase from Candida rugosa on cellulose and its influence on lipolytic activity. Enzyme Microb Technol 14: 748-754
7. Halling PJ (1994) Thermodynamic predictions for biocatalysis in nonconventional media: theory, tests, and recommendations for experimental design and analysis. Enzyme Microb Technol 16: 178-206
8. Holmquist M, Martinelle M, Berglund P, Clausen IG, Patkar S, Svendsen A, Hult K (1993) Lipases from Rhizomucor miehei and Humicola lanuginosa: modification of the lid covering the active site alters enantioselectivity. J Protein Chem 12: 747-755
9. Lavayre J, Baratti J (1982) Preparation and properties of immobilized lipases. Biotechnol Bioeng 24: 1007-1013
10. Malcata FX, Reyes HR, Garcia HS, Hill CG Jr, Amundson CH (1990) Immobilized lipase reactors for modification of fats and oils. A review. J Am Oil Chem Soc 67: 890-910
11. Ottolina G, Carrea G, Riva S, Sartore L, Veronese FM (1992) Effect of the enzyme form on the activity, stability and enantioselectivity of lipoprotein lipase in toluene. Biotechnol Lett 14: 947-952
12. Pencreac'h G, Baratti J (1996) Hydrolysis of p-nitrophenyl palmitate in n-heptane by the Pseudomonas cepacia lipase: a simple test for the determination of lipase activity in organic media. Enzyme Microbiol Technol 18: 417-422
13. Pencreac'h G, Leullier M, Baratti J (1997) Properties of free and immobilized lipase from Pseudomonas cepacia. Biotechnol Bioeng (in press)
14. Reslow M, Adlercreutz P, Mattiasson B (1988) On the importance of the support material for bioorganic synthesis influence of water partition between solvent, enzyme and solid support in water-poor reaction media. Eur J Biochem 172: 573-578
15. Valivety RH, Halling PJ, Macrae AR (1992) Reaction rate with suspended lipase catalyst shows similar dependence on water activity in different organic solvents. Biochim Biophys Acta 1118: 218-222
16. Valivety RH, Halling PJ, Peilow AD, Macrae AR (1994) Relationship between water activity and catalytic activity of lipases in organic media - effects of supports, loading and enzyme preparation. Eur J Biochem 222: 461-166
17. Yamane T, Ichiryu T, Nagala M, Ueno A, Shimizu S (1990) Intramolecular esterification by lipase powder in microaqueous benzene: factors affecting activity of pure enzyme. Biotechnol Bioeng 36: 1063-1069
18. Yang H, Cao SG, Ma L, Ding ZT, Liu SD, Cheng VH (1994) A new kind of immobilized lipase in organic solvent and its structure model. Biochem Biophys Res Commun 200: 83-88