Heterogeneity of primer extension products in asymmetric PCR is due both to cleavage by a structure-specific exo/endonuclease activity of DNA polymerases and to premature stops

Proceedings of the National Academy of Sciences of the United States of America

Volumn 93, Issue 7, 1996, Pages 2724-2728

  Tombline, Gregory ^a,c   Bellizzi, Dina ^b   Sgaramella, Vittorio ^b  

^a ROCKEFELLER UNIVERSITY   (United States)

^b UNIVERSITY OF CALABRIA   (Italy)

^c THOMAS JEFFERSON UNIVERSITY   (United States)

Author keywords

elongation; eubacterial DNA polymerases; hairpins; PCR plateau

Indexed keywords

DNA POLYMERASE;

ARTICLE; DNA SYNTHESIS; DNA TEMPLATE; ENZYME ACTIVITY; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; STRUCTURE ACTIVITY RELATION; TEMPERATURE MEASUREMENT; THERMUS AQUATICUS; THERMUS THERMOPHILUS;

BASE SEQUENCE; DNA PRIMERS; DNA-DIRECTED DNA POLYMERASE; ENDODEOXYRIBONUCLEASES; ESCHERICHIA COLI; EXODEOXYRIBONUCLEASES; MOLECULAR SEQUENCE DATA; NUCLEIC ACID CONFORMATION; PLASMIDS; POLYMERASE CHAIN REACTION; SUBSTRATE SPECIFICITY; TAQ POLYMERASE; TEMPLATES, GENETIC; THERMUS; THERMUS THERMOPHILUS;

EID: 0029981239     PISSN: 00278424     EISSN: None     Source Type: Journal    
DOI: 10.1073/pnas.93.7.2724     Document Type: Article

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