Use of surface plasmon resonance for real-time measurements of the global conformational transition in human phenylalanine hydroxylase in response to substrate binding and catalytic activation

Analytical Biochemistry

Volumn 294, Issue 2, 2001, Pages 95-101

  Flatmark, Torgeir ^a   Stokka, Anne Jorunn ^a   Berge, Sissel V ^a  

^a UNIVERSITY OF BERGEN   (Norway)

Author keywords

Affinity; Association and dissociation rate; Biacore; Binding; Biosensor; Phenylalanine hydroxylase; Surface plasmon resonance

Indexed keywords

AMINO ACIDS; BIOSENSORS; DISSOCIATION; ENZYMES; PLASMONS; RESONANCE; TIME MEASUREMENT;

AFFINITY; BIACORE; BINDING; DISSOCIATION RATES; PHENYLALANINE HYDROXYLASE;

SURFACE PLASMON RESONANCE;

PHENYLALANINE 4 MONOOXYGENASE; PTERIN DERIVATIVE; TETRAHYDROBIOPTERIN;

ARTICLE; BINDING AFFINITY; BIOSENSOR; CHEMICAL BINDING; CONFORMATIONAL TRANSITION; ENZYME ACTIVATION; HALF LIFE TIME; KINETICS; MEASUREMENT; PRIORITY JOURNAL; STEADY STATE; SURFACE PLASMON RESONANCE;

EID: 0035879403     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.2001.5163     Document Type: Article

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