Microheterogeneity of recombinant human phenylalanine hydroxylase as a result of nonenzymatic deamidations of labile amide containing amino acids: Effects on catalytic and stability properties

European Journal of Biochemistry

Volumn 267, Issue 20, 2000, Pages 6302-6310

  Solstad, T ^a   Flatmark, T ^a  

^a UNIVERSITY OF BERGEN   (Norway)

Author keywords

Deamidation; Escherichia coli; Heterologous expression; Microheterogeneity; Phenylalanine hydroxylase

Indexed keywords

AMIDE; AMINO ACID; PHENYLALANINE 4 MONOOXYGENASE; RECOMBINANT ENZYME;

ARTICLE; CATALYSIS; DEAMINATION; ENZYME ACTIVITY; ENZYME INDUCTION; ENZYME POLYMORPHISM; ENZYME STABILITY; ESCHERICHIA COLI; ISOELECTRIC FOCUSING; NONHUMAN; PH; PRIORITY JOURNAL; PROTEIN EXPRESSION; TEMPERATURE DEPENDENCE; TWO DIMENSIONAL ELECTROPHORESIS;

AMIDES; AMMONIA; CATALYSIS; CLONING, MOLECULAR; ENZYME STABILITY; ESCHERICHIA COLI; HUMAN; ISOELECTRIC FOCUSING; KINETICS; PHENYLALANINE HYDROXYLASE; PHOSPHORYLATION; RECOMBINANT PROTEINS; SUPPORT, NON-U.S. GOV'T; TRYPSIN;

ESCHERICHIA COLI;

EID: 0033771865     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.2000.01715.x     Document Type: Article

References (30)

1. The phenylalanine hydroxylating system from mammalian liver
2. Purification and some physical properties of phenylalanine hydroxylase from rat liver
3. Phenylalanine hydroxylase from monkey liver
4. Phenylalanine 4-monooxygenase from human liver
5. Molecular characterization of disease related mutant forms of human phenylalanine hydroxylase and tyrosine hydroxylase
6. Structural insight into the aromatic amino acid hydroxylases and their disease related mutant forms
7. Two apparent molecular weight forms of human and monkey phenylalanine hydroxylase are due to phosphorylation
8. Recombinant human phenylalanine hydroxylase is a substrate for the ubiquitin-conjugating enzyme system
9. Nonenzymatic deamidation of asparaginyl and glutaminyl residues in proteins
10. Expression of recombinant human phenylalanine hydroxylase as a fusion protein in Escherichia coli circumvents proteolytic degradation by host cell proteases. Isolation and characterization of the wild-type enzyme
11. Structure/function relationships in human phenylalanine hydroxylase. Effect of terminal deletions on the oligomerization, activation and cooperativity of substrate binding to the enzyme
12. Partial characterization and three-dimensional-structural localization of eight mutations in exon 7 of the human phenylalanine hydroxylase gene associated with phenylketonuria
13. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
14. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
15. Phosphorylation of recombinant human phenylalanine hydroxylase: Effect on catalytic activity, substrate activation and protection against non-specific cleavage of the fusion protein by restriction protease
16. Is substrate inhibition a consequence of allostery in aspartate transcarbamylase?
17. On the heterogeneity of beef heart cytochrome c. III. A kinetic study of the non-enzymatic deamidation of the main subfractions (Cy I-Cy III)
18. Sequence dependent deamidation rates for model peptides of histone IV
19. Sequence dependent deamidation rates for model peptides of cytochrome c
20. Deamidations of glutaminyl and asparaginyl residues in peptides and proteins
21. PKU mutation (D143G) associated with an apparent high residual enzyme activity: Expression of a kinetic variant form of phenylalanine hydroxylase in three different systems
22. Cryptic amidase active sites catalyzes deamidation in proteins
23. Deamidation of asparagine residues in a recombinant serine hydroxymethyltransferase
24. Crystal structure of the catalytic domain of the human phenylalanine hydroxylase reveals the structural basis for phenylketonuria
25. Structure of tetrameric phenylalanine hydroxylase and its implications for phenylketonuria
26. Multiple molecular forms of bovine heart cytochrome c. V. A comparative study of their physicochemical properties and their reactions in biological systems
27. Multiple forms of cytochrome c in the rat. Precursor-product relationship between the main component Cy I and the minor components Cy II and Cy III in vivo
28. Substrate inhibition
29. Crystal structure and site-specific mutagenesis of pterin-bound human phenylalanine hydroxylase