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Journal of Colloid and Interface Science

Volumn 238, Issue 2, 2001, Pages 333-339

Microcalorimetric study of the effect of hexa-histidine tag and denaturant on the interaction mechanism between protein and metal-chelating gel

(3) Lin, Fu Yung a Chen, Wen Yih a Chen, Hsiu Mei b

a NATIONAL CENTRAL UNIVERSITY (Taiwan)

b NATIONAL TAIWAN UNIVERSITY OF SCIENCE AND TECHNOLOGY (Taiwan)

Author keywords

Adsorption enthalpy; Immobilized metal ion affinity chromatography, IMAC; Isothermal titration calorimetry, ITC; Poly histidine tag; Protein adsorption; Schistosoma japonicum glutathione S transferase

Indexed keywords

ADSORBENTS; CALORIMETRY; CHELATION; CHROMATOGRAPHIC ANALYSIS; GELS; METALS; RESINS;

DENATURANTS;

PROTEINS;

ADSORBENT; COBALT; GLUTATHIONE TRANSFERASE; HISTIDINE DERIVATIVE; METAL; PROTEIN;

ADSORPTION; ARTICLE; BINDING AFFINITY; CALORIMETRY; CARBOXY TERMINAL SEQUENCE; CHELATION; CHROMATOGRAPHY; COORDINATION; DENATURATION; ENTHALPY; GEL; HEAT; MICROCALORIMETRY; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN IMMOBILIZATION; PROTEIN INTERACTION; SCHISTOSOMA JAPONICUM; TEMPERATURE DEPENDENCE; THERMODYNAMICS; TITRIMETRY;

EID: 0035877917 PISSN: 00219797 EISSN: None Source Type: Journal
DOI: 10.1006/jcis.2001.7526 Document Type: Article

Times cited : (8)

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