Cx32 but not Cx26 is associated with tight junctions in primary cultures of rat hepatocytes

Experimental Cell Research

Volumn 263, Issue 2, 2001, Pages 193-201

  Kojima, Takashi ^a   Kokai, Yasuo ^a   Chiba, Hideki ^a   Yamamoto, Masao ^b   Mochizuki, Yohichi ^a   Sawada, Norimasa ^a  

^a SAPPORO MEDICAL UNIVERSITY SCHOOL OF MEDICINE   (Japan)

^b Kure University   (Japan)

Author keywords

Gap junctions; Primary rat hepatocytes; Tight junctions

Indexed keywords

CLAUDIN; GAP JUNCTION PROTEIN; OCCLUDIN; PROTEIN CX26; PROTEIN CX32; PROTEIN ZO1; PROTEIN ZO2; SPHINGOMYELIN; UNCLASSIFIED DRUG;

ANIMAL CELL; ANIMAL TISSUE; APICAL MEMBRANE; ARTICLE; BASOLATERAL MEMBRANE; CELL DIFFERENTIATION; CELL MEMBRANE; CELL POLARITY; CONTROLLED STUDY; FREEZE FRACTURE; GAP JUNCTION; IMMUNOPRECIPITATION; LIVER CELL CULTURE; MALE; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; RAT; TIGHT JUNCTION; WESTERN BLOTTING;

EID: 0035864383     PISSN: 00144827     EISSN: None     Source Type: Journal    
DOI: 10.1006/excr.2000.5103     Document Type: Article

References (62)

1. c-AMP increases junctional conductance and stimulates phosphorylation of the 27 KDa principal gap junction polypeptide
2. Hepatocyte gap junctions are permeable to the second messenger, inositol 1,4,5-triphosphate and to calcium ions
3. The gap junction communication channel
4. Junctional intercellular communication and the control of growth
5. Gap junctions: New tools, new answers, new questions
6. Connexins and glucagon receptors during development of rat hepatic acinus
7. Role of connexin genes in growth control
8. Cell-cell communication in carcinogenesis
9. Vitamin A-induced mucous metaplasia. An in vitro system for modulating tight and gap junction differentiation
10. Intercellular junctional complexes of the rat seminiferous tubules: A freeze-fracture study
11. Establishment of hepatic cell polarity in the rat hepatoma-human fibroblast hybrid WIF-B9
12. Changes in cellular distribution of connexin 32 and 26 during formation of gap junctions in primary cultures of adult rat hepatocytes
13. Different changes in expression and function of connexin 26 and connexin 32 during DNA synthesis and redifferentiation in primary rat hepatocytes using a DMSO culture system
14. Identification of ZO-1: A high molecular weight polypeptide associated with the tight junction (zonula occludens) in a variety of epithelia
15. Characterization of ZO-1, a protein component of the tight junction from mouse liver and Madin-Darby canine kidney cells
16. The gap junction protein connexin43 interacts with the second PDZ domain of the zona occludens-1 protein
17. Direct association of the gap junction protein connexin-43 with ZO-1 in cardiac myocytes
18. Breaking through the tight junction barrier
19. Structure, function, and regulation of cellular tight junctions
20. Further observations on the fine structure of freeze-cleaved tight junctions
21. Occludin: A novel integral membrane protein localizing at tight junctions
22. Claudin-1 and -2: Novel integral membrane proteins localizing at tight junctions with no sequence similarity to occludin
23. Claudin multigene family encoding four-transmembrane domain protein components of tight junction strands
24. Junctional adhesion molecule, a novel member of the immunoglobulin superfamily that distributes at intercellular junctions and modulates monocyte transmigration
25. Molecular characterization and tissue distribution of ZO-2, a tight junction protein homologous to ZO-1 and the Drosophila discs-large tumor suppressor protein
26. ZO-3, a novel member of the MAGUK protein family found at the tight junction, interacts with ZO-1 and occludin
27. Monoclonal antibody 7H6 reacts with a novel tight junction-associated protein distinct from ZO-1, cingulin and ZO-2
28. Cingulin, a new peripheral component of fight junctions
29. Symplekin, a novel type of tight junction plaque protein
30. Expression and polarized targeting of a rab3 isoform in epithelial cells
31. The Ras target AF-6 interacts with ZO-1 and serves as a peripheral component of tight junctions in epithelial cells
32. An atypical PKC directly associates and colocalizes at the epithelial tight junction with ASIP, a mammalian homologue of Caenorhabditis elegans polarity protein PAR-3
33. Direct association of occludin with ZO-1 and its possible involvement in the localization of occludin at tight junctions
34. Characterization of ZO-2 as a MAGUK family member associated with tight as well as adherens junctions with a binding affinity to occludin and alpha catenin
35. Direct binding of three tight junction-associated MAGUKs, ZO-1, ZO-2, and ZO-3, with the COOH termini of claudins
36. Two homologous protein components of hepatic gap junctions
37. Comparative characterization of the 21-kD and 26-kD gap junction proteins in murine liver and cultured hepatocytes
38. Gap junctions in liver: Composition, function, and regulation
39. Reappearance and long-term maintenance of connexin 32 in proliferated adult rat hepatocytes: Use of serum-free L-15 medium supplemented with EGF and DMSO
40. Induction and regulation of connexin26 by glucagon in primary cultures of adult rat hepatocytes
41. Preparation of isolated rat liver cells
42. Primary cultures of parenchymal liver cells on collagen membranes: Morphological and biological observations
43. Electron microscope observations on the formation of primitive villi in rat small intestine with special reference to intercellular junctions
44. Functional dissociation of paracellular permeability and transepithelial electrical resistance and disruption of the apical-basolateral intramembrane diffusion barrier by expression of a mutant tight junction membrane protein
45. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
46. Connections between cells of the developing squid as revealed by electrophysiological methods
47. Junctional intercellular communication: The cell-to-cell membrane channel
48. Gap junctions and cell polarity: Connexin32 and connexin43 expressed in polarized thyroid epithelial cells assemble into separate gap junctions, which are located in distinct regions of the lateral plasma membrane domain
49. Switch in gap junction protein expression is associated with selective changes in junctional permeability during keratinocyte differentiation
50. Modulation of connexins during differentiation of oval cells into hepatocytes
51. Formation of three-dimensional thyroid follicle-like structures by polarized FRT cells made communication competent by transfection and stable expression of the connexin-32 gene
52. Induction of tight junctions in human connexin 32 (hCx32)-transfected mouse hepatocytes: Connexin 32 interacts with occludin
53. A single gene product, claudin-1 or -2, reconstitutes tight junction strands and recruits occludin in fibroblasts
54. A dominant mutant of occludin disrupts tight junction structure and function
55. The coiled-coil domain of occludin can act to organize structural and functional elements of the epithelial tight junction
56. Tight junctions are membrane microdomains
57. Calmodulin directly participates in chemical gating of gap junction channels
58. Manner of interaction of heterogeneous claudin species within and between tight junction strands
59. The function of tight junctions in maintaining differences in lipid composition between the apical and basolateral cell surface domains of MDCK cells
60. Role of tight junctions in establishing and maintaining cell polarity
61. Tight junctions and the experimental modifications of lipid content
62. Uncoupling of gate and fence functions of MDCK cells by the actin depolymerizing reagent mycalolide B