A dominant mutant of occludin disrupts tight junction structure and function

Journal of Cell Science

Volumn 112, Issue 12, 1999, Pages 1879-1888

  Bamforth, Simon D ^a,b   Kniesel, Uwe ^c   Wolburg, Hartwig ^c   Engelhardt, Britta ^a   Risau, Werner ^a  

^a MAX PLANCK INSTITUTE FOR HEART AND LUNG RESEARCH   (Germany)

^b UNIVERSITY OF OXFORD   (United Kingdom)

^c UNIVERSITY OF TÜBINGEN   (Germany)

Author keywords

Occludin; Permeability; Tight junction; Tight junction strand

Indexed keywords

MEMBRANE PROTEIN; OCCLUDIN;

ANIMAL CELL; ARTICLE; CELL JUNCTION; CELL LINE; CELL MEMBRANE PERMEABILITY; CONTROLLED STUDY; DIFFUSION; ELECTRIC RESISTANCE; ELECTRON MICROSCOPY; EPITHELIUM CELL; MOLECULAR WEIGHT; MOUSE; NONHUMAN; PERMEABILITY BARRIER; PRIORITY JOURNAL; TIGHT JUNCTION;

ANIMALS; CELL LINE; ELECTRIC IMPEDANCE; EPITHELIAL CELLS; FREEZE FRACTURING; GENES, DOMINANT; MEMBRANE PROTEINS; MICE; MICROSCOPY, ELECTRON; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA; STRUCTURE-ACTIVITY RELATIONSHIP; TIGHT JUNCTIONS; TUMOR CELLS, CULTURED;

ANIMALIA; MURINAE; PHOCIDAE;

EID: 0032769698     PISSN: 00219533     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (43)

1. Ando-Akatsuka, Y., Saitou, M., Hirase, T., Kishi, M., Sakakibara, A., Itoh, M., Yonemura, S., Furuse, M. and Tsukita, S. (1996). Interspecies diversity of the occludin sequence: cDNA cloning of human, mouse, dog, and rat-kangaroo homologues. J. Cell Biol. 133, 43-47.
2. Balda, M. S., Whitney, J. A., Flores, C., Gonzalez, S., Cereijido, M. and Matter, K. (1996). Functional dissociation of paracellular permeability and transepithelial electrical resistance and disruption of the apical-basolateral intramembrane diffusion barrier by expression of a mutant tight junction membrane protein. J. Cell Biol. 134, 1031-1049.
3. Chen, C. and Okayama, H. (1987). High-efficiency transformation of mammalian cells by plasmid DNA. Mol. Cell. Biol. 7, 2745-2752.
4. Chen, Y. H., Merzdorf, C., Paul, D. L. and Goodenough, D. A. (1997). COOH terminus of occludin is required for tight junction barrier function in early Xenopus embryos. J. Cell Biol. 138, 891-899.
5. Chomczynski, P. and Sacchi, N. (1987). Single-step method of RNA isolation by acidguanidium thiocyanate-phenol-chloroform extraction. Annal. Biochem. 162, 156-159.
6. Citi, S., Sabanay, H., Jakes, R., Geiger, B. and Kendrick-Jones, J. (1988). Cingulin, a new peripheral component of tight junctions. Nature 333, 272-275.
7. Claude, P. (1978). Morphological factors influencing transepithelial permeability: a model for the resistance of the zonula occludens. J. Membr. Biol. 39, 219-232.
8. Drenckhahn, D. and Dermietzel, R. (1988). Organization of the actin filament cytoskeleton in the intestinal brush border: a quantitative and qualitative immunoelectron microscope study. J. Cell Biol. 107, 1037-1048.
9. Fanning, A. S., Jameson, B. J., Jesaitis, L. A. and Anderson. J. M. (1998). The tight junction protein ZO-1 establishes a link between the transmembrane protein occludin and the actin cytoskeleton. J. Biol. Chem. 273, 29745-29753.
10. Farquhar, M. G. and Palade, G. E. (1963). Junctional complexes in various epithelia. J. Cell Biol. 17, 375-412.
11. Fujimoto, K. (1995). Freeze-fracture replica electron microscopy combined with SDS digestion for cytochemical labeling of integral membrane proteins. Application to the immunogold labeling of intercellular junctional complexes. J. Cell Sci. 108, 3443-3449.
12. Furuse, M., Hirase, T., Itoh, M., Nagafuchi, A., Yonemura, S., Tsukita, S. and Tsukita, S. (1993). Occludin: a novel integral membrane protein localizing at tight junctions. J. Cell Biol. 123, 1777-1788.
13. Furuse, M., Itoh, M., Hirase, T., Nagafuchi, A., Yonemura, S., Tsukita, S. and Tsukita, S. (1994). Direct association of occludin with ZO-1 and its possible involvement in the localization of occludin at tight junctions. J. Cell Biol. 127, 1617-1626.
14. Furuse, M., Fujimoto, K., Sato, N., Hirase, T., Tsukita, S. and Tsukita, S. (1996). Overexpression of occludin, a tight junction-associated integral membrane protein, induces the formation of intracellular multilamellar bodies bearing tight junction-like structures. J. Cell Sci. 109, 429-435.
15. Furuse, M., Fujita, K., Hiiragi, T., Fujimoto, K. and Tsukita, S. (1998a). Claudin-1 and -2: novel integral membrane proteins localizing at tight junctions with no sequence similarity to occludin. J. Cell Biol. 141, 1539-1550.
16. Furuse, M., Sasaki, H., Fujimoto, K. and Tsukita, S. (1998b). A single gene product, claudin-1 or -2, reconstitutes tight junction strands and recruits occludin in fibroblasts. J. Cell Biol. 143, 391-401.
17. Gumbiner, B., Lowenkopf, T. and Apatira, D. (1991). Identification of a 160-kDa polypeptide that binds to the tight junction protein ZO-1. Proc. Nat. Acad. Sci. USA 88, 3460-3464.
18. Gumbiner, B. M. (1993). Breaking through the tight junction barrier. J. Cell Biol. 123, 1631-1633.
19. Haskins, J., Gu, L. J., Wittchen, E. S., Hibbard, J. and Stevenson, B. R. (1998). ZO-3, a novel member of the MAGUK protein family found at the tight junction, interacts with ZO-1 and occludin. J. Cell Biol. 141, 199-208.
20. Kniesel, U., Reichenbach, A., Risau, W. and Wolburg, H. (1994). Quantification of tight junction complexity by means of fractal analysis. Tissue & Cell 26, 901-912.
21. Knowles, M. A. and Franks, L. M. (1977). Stages in neoplastic transformation of adult epithelial cells by 7, 12-dimethylbenz(a)anthracene in vitro. Cancer Res. 37, 3917-3924.
22. Knowles, M. A. and Franks, L. M. (1978). Ultrastructural and biological markers of neoplastic change in adult mouse epithelial cells transformed in vitro. Br. J. Cancer 37, 603-611.
23. Lane, N. J., Revest, V. A., Whytock, S. and Abbott, N. J. (1995). Fine-structural investigation of rat brain microvascular endothelial cells: tight junctions and vesicular structures in freshly isolated and cultured preparations. J. Neurocytol. 24, 347-360.
24. Madara, J. L. (1987). Intestinal absorptive cell tight junctions are linked to cytoskeleton. Am. J. Physiol. 253, C171-C175.
25. Madara, J. L. (1998). Regulation of the movement of solutes across tight junctions. Ann. Rev. Physiol. 60, 143-159.
26. Mandel, L. J., Bacallao, R. and Zampighi, G. (1993). Uncoupling of the molecular fence and paracellular gate functions in epithelial tight junctions. Nature 361, 552-555.
27. Markowitz, D., Goff, S. and Bank, A. (1988). A safe packaging line for gene transfer: separating viral genes on two different plasmids. J. Virol. 62, 1120-1124.
28. Martin-Padura, I., Lostaglio, S., Schneemann, M., Williams, L., Romano, M., Fruscella, P., Panzeri, C., Stoppacciaro, A., Ruco, L., Villa, A., Simmons, D. and Dejana, E. (1998). Junctional adhesion molecule, a novel member of the immunoglobulin superfamily that distributes at intercellular junctions and modulates monocyte transmigration. J. Cell Biol. 142, 117-127.
29. Matter, K. and Balda, M. S. (1998). Biogenesis of tight junctions - the C-terminal domain of occludin mediates basolateral targeting. J. Cell Sci. 111, 511-519.
30. McCarthy, K. M., Skare, I. B., Stankewich, M. C., Furuse, M., Tsukita, S., Rogers, R. A., Lynch, R. D. and Schneeberger, E. E. (1996). Occludin is a functional component of the tight junction. J. Cell Sci. 109, 2287-2298.
31. Mitic, L. L. and Anderson, J. M. (1998). Molecular architecture of tight junctions. Annu. Rev. Physiol. 60, 121-142.
32. Morgenstern, J. P. and Land, H. (1990). Advanced mammalian gene transfer: high titre retroviral vectors with multiple drug selection markers and a complementary helper-free packaging cell line. Nucl. Acid Res. 18, 3587-3596.
33. Mühleisen, H., Wolburg, H. and Betz, E. (1989). Freeze-fracture analysis of endothelial cell membranes in rabbit carotid arteries subjected to short-term atherogenic stimuli. Virch. Arch. B Cell Pathol. 56, 413-417.
34. Saitou, M., Fujimoto, K., Doi, Y., Itoh, M., Fujimoto, T., Furuse, M., Takano, H., Noda, T. and Tsukita, S. (1998). Occludin-deficient embryonic stem cells can differentiate into polarized epithelial cells bearing tight junctions. J. Cell Biol. 141, 397-408.
35. Schneeberger, E. E. and Lynch, R. D. (1992). Structure, function, and regulation of cellular tight junctions. Am. J. Physiol. 262, L 647-L 661.
36. Schnürch, H. and Risau, W. (1993). Expression of tie-2, a member of a novel family of receptor tyrosine kinases, in the endothelial cell lineage. Development. 119, 957-968.
37. Simionescu, M., Ghinea, N., Fixman, A., Lasser, M., Kukes, L., Simionescu, N. and Palade, G. E. (1988). The cerebral microvasculature of the rat: structure and luminal surface properties during early development. J. Submicrosc. Cytol. 20, 243-261.
38. Stevenson, B. R., Siliciano, J. D., Mooseker, M. S. and Goodenough, D. A. (1986). Identification of ZO-1: a high molecular weight polypeptide associated with the tight junction (zonula occludens) in a variety of epithelia. J. Cell Biol. 103, 755-766.
39. Stevenson, B. R. and Begg, D. A. (1994). Concentration-dependent effects of cytochalasin D on tight junctions and actin filaments in MDCK epithelial cells. J. Cell Sci. 107, 367-375.
40. Van Itallie, C. M. and Anderson, J. M. (1997). Occludin confers adhesiveness when expressed in fibroblasts. J. Cell Sci. 110, 1113-1121.
41. van Meer, G. and Simons, K. (1986). The function of tight junctions in maintaining differences in lipid composition between the apical and the basolateral cell surface domains of MDCK cells. EMBO J. 5, 1455-1464.
42. Wolburg, H., Neuhaus, J., Kniesel, U., Krauss, B., Schmid, E. M., Öcalan, M., Farrell, C. and Risau, W. (1994). Modulation of tight junction structure in blood-brain barrier endothelial cells. Effects of tissue culture, second messengers and cocultured astrocytes. J. Cell Sci. 107, 1347-1357.
43. Wong, V. and Gumbiner, B. M. (1997). A synthetic peptide corresponding to the extracellular domain of occludin perturbs the tight junction permeability barrier. J. Cell Biol. 136, 399-409.