Establishment of variant PC12 subclones deficient in stimulation-secretion coupling

Biochimica et Biophysica Acta - Molecular Cell Research

Volumn 1499, Issue 3, 2001, Pages 180-190

  Shoji Kasai, Yoko ^a   Morishima, Mieko ^b,c   Kuwahara, Reiko ^a   Kondo, Shunzo ^a   Itakura, Makoto ^a   Takahashi, Masami ^a  

^a MITSUBISHI KASEI INST OF LIFE SCI   (Japan)

^b TOKYO UNIVERSITY OF PHARMACY AND LIFE SCIENCES   (Japan)

^c OSAKA UNIVERSITY GRADUATE SCHOOL OF MEDICINE   (Japan)

Author keywords

Ca2+ channel; Catecholamine transporter; Dopamine; Exocytosis; PC12 cell; SNARE protein

Indexed keywords

CALCIUM CHANNEL L TYPE; CALCIUM ION; CELL PROTEIN; DOPAMINE; FURA 2; IONOMYCIN; MUNC18 PROTEIN; NICARDIPINE; POTASSIUM ION; PROTEIN SEC 6; RAB PROTEIN; SNARE PROTEIN; SYNAPTOBREVIN; SYNAPTOPHYSIN; SYNAPTOSOMAL ASSOCIATED PROTEIN 25; SYNTAXIN; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CALCIUM TRANSPORT; CATECHOLAMINE UPTAKE; CELL CLONE; CELL MEMBRANE DEPOLARIZATION; CELL STRAIN; CONTROLLED STUDY; DOPAMINE RELEASE; ELECTRON MICROSCOPY; EXOCYTOSIS; FLUOROMETRY; IMMUNOBLOTTING; NEUROTRANSMITTER RELEASE; NONHUMAN; PHEOCHROMOCYTOMA CELL; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN SYNTHESIS; RAT; STIMULUS SECRETION COUPLING;

AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; CALCIUM; CLONE CELLS; DNA PRIMERS; DOPAMINE; EXOCYTOSIS; GENE EXPRESSION; IONOMYCIN; IONOPHORES; MEMBRANE POTENTIALS; MICROSCOPY, ELECTRON; MOLECULAR SEQUENCE DATA; MUTATION; NERVE TISSUE PROTEINS; NEUROTRANSMITTER AGENTS; PC12 CELLS; POTASSIUM; RATS;

EID: 0035862435     PISSN: 01674889     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0167-4889(00)00103-8     Document Type: Article

References (40)

1. Mechanisms of intracellular protein transport
2. A fusion of new ideas
3. PC12 pheochromocytoma cultures in neurobiological research
4. Control of exocytosis in adrenal chromaffin cells
5. PC12 variants deficient in catecholamine transport
6. Overall lack of regulated secretion in a PC12 variant cell clone
7. Clonal variants of PC12 pheochromocytoma cells with defects in cAMP-dependent protein kinases induce ornithine decarboxylase in response to nerve growth factor but not to adenosine agonists
8. Nerve growth factor-mediated stimulation of tyrosine hydroxylase activity in a clonal rat pheochromocytoma cell line
9. Comparison of exocytotic mechanisms between acetylcholine- and catecholamine-containing vesicles in rat pheochromocytoma cells
10. Neurotransmitter release from synaptotagmin-deficient clonal variants of PC12 cells
11. Neurite outgrowth in PC12 cells deficient in GAP-43
12. Real-time monitoring of the secretory function of culture adrenal chromaffin cells
13. 2+-independent release of neurotransmitter induced by α-latrotoxin
14. 2+ indicators with greatly improved fluorescence properties
15. Identification of protein receptor for Clostridium botulinum type B neurotoxin in rat brain synaptosomes
16. 2+/calmodulin-dependent protein kinase II phosphorylate a novel 58-kDa protein in synaptic vesicles
17. Immunological search for molecules specific to subtypes of neurons
18. HPC-1 is associated with synaptotagmin and ω-conotoxin receptor
19. Expression and complex formation of soluble N-ethyl-maleimide-sensitive factor attachment protein (SNAP) receptors in clonal rat endocrine cells
20. Transient transfection studies of secretion in bovine chromaffin cells and PC12 cells
21. Transfection analysis of functional roles of complexin I and II in the exocytosis of two deferent types of secretory vesicles
22. 2+ entry pathways in neuronal preparations
23. Calcium antagonists high-affinity binding and inhibition of calcium transport in a clonal cell line
24. L-type calcium channels and GSK-3 regulate the activity of NF-Atc4 in hippocampal neurons
25. Distinct functions of nuclear and cytoplasmic calcium in the control of gene expression
26. L-type voltage-sensitive calcium channel activation stimulates gene expression by a serum response factor-dependent pathway
27. Proteins of synaptic vesicles involved in exocytosis and membrane recycling
28. Calcium-dependent transmitter secretion reconstituted in Xenopus oocytes: Requirement for synaptophysin
29. Antibodies to synaptophysin interfere with transmitter secretion at neuromuscular synapses
30. Overexpression of synaptophysin enhances neurotransmitter secretion at Xenopus neuromuscular synapses
31. P29: A novel tyrosine-phosphorylated membrane protein present in small clear vesicles of neurons and endocrine cells
32. Structure of synaptogyrin (p29) defines novel synaptic vesicle protein
33. Mice lacking synaptophysin reproduce and form typical synaptic vesicles
34. Synaptophysin, a major synaptic vesicle protein, is not essential for neurotransmitter release
35. Initial docking of ER-derived vesicles requires Uso1p and Ypt1p but is independent of SNARE proteins
36. Genetic and electrophysiological studies of Drosophila syntaxin-1A demonstrate its role in nonneuronal secretion and neurotransmission
37. Syntaxin and synaptobrevin function downstream of vesicle docking in Drosophila
38. Exocytosis: Proteins and perturbations
39. Expression cloning of a cocaine- and antidepressant-sensitive human noradrenaline transporter
40. The rat norepinephrine transporter: Molecular cloning from PC12 cells and functional expression