Exocytosis: Proteins and perturbations

Annual Review of Pharmacology and Toxicology

Volumn 36, Issue , 1996, Pages 659-701

  Augustine, George J ^a   Burns, Marie E ^a   DeBello, William M ^a   Pettit, Diana L ^a   Schweizer, Felix E ^a  

^a DUKE UNIVERSITY MEDICAL CENTER   (United States)

Author keywords

calcium; membrane fusion; secretion

Indexed keywords

PROTEIN;

CELL MEMBRANE; DYNAMICS; EXOCYTOSIS; GENETICS; HUMAN; MOLECULAR MODEL; NONHUMAN; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; REVIEW;

EID: 0030005561     PISSN: 00664251     EISSN: None     Source Type: Book Series    
DOI: 10.1146/annurev.pa.36.040196.003303     Document Type: Review

References (273)

1. Kelly RB. 1985. Pathways of protein secretion in eukaryotes. Science 230:25-32
2. Schweizer FE, Betz H, Augustine GJ. 1995. From vesicle docking to endocytosis: intermediate reactions of exocytosis. Neuron 14 689-96
3. 2+ activated secretion Ann. NY Acad. Sci. 710:328-32
4. Scheller RH. 1995. Membrane trafficking in the presynaptic nerve terminal Neuron 14.893-97
5. Sudhof TC. 1995 The synaptic vesicle cycle a cascade of protein-protein interactions. Nature 375 645-53
6. Ferro-Novick S, Jahn R. 1994. Vesicle fusion from yeast to man. Nature 370: 191-93
7. DiAntonio A, Parfitt KD, Schwarz TL. 1993 Synaptic transmission persists in synaptotagmin mutants of Drosophila. Cell 73:1281-90
8. 2+-activated neurotransmitter release. Cell 74: 1125-34
9. Nonet ML, Grundahl K, Meyer BJ, Rand JB. 1993. Synaptic function is impaired but not eliminated in C. elegans mutants lacking synaptotagmin. Cell 73:1291-305
10. Silva AJ, Stevens CF, Tonegawa S, Wang Y. 1992 Deficient hippocampal long-term potentiation in α-calcium-calmodulin kinase II mutant mice. Science 257:201-6
11. Rosahl TW, Geppert M, Spillane D, Herz J, Hammer RE, et al. 1993. Short-term synaptic plasticity is altered in mice lacking synapsin I. Cell 75: 661-70
12. Geppert M, Bolshakov VY, Siegelbaum SA, Takei K, De Camilli P, et al. 1994. The role of Rab3A in neurotransmitter release. Nature 369:493-97
13. Geppert M, Ullrich B, Green DG, Takei K, Daniels L, et al. 1994. Synaptic targeting domains of synapsin I revealed by transgenic expression in photoreceptor cells. EMBO J. 13:3720-27
14. Mayford M, Wang J, Kandel ER, O'Dell TJ. 1995. CaMKII regulates the frequency-response function of hippocampal synapses for the production of both LTD and LTP. Cell 81:891-904
15. Sambrook J, Fritsch EF, Maniatis T. 1989. Expression of cloned genes in cultured mammalian cells. In Molecular Cloning, ed. N Ford, C Nolan, M Ferguson, M Ockler, pp. 16.30-16.37. Cold Spring Harbor, NY: Cold Spring Harbor Lab. Press
16. Neumann E, Schaefer-Ridder M, Wang Y, Hofschneider PH. 1982. Gene transfer into mouse lyoma cells by electroporation. EMBO J. 1:841-45
17. Geller AI, Breakefield XO. 1988. A defective HSV-1 vector expresses E. coli β-galactosidase in cultured periperal neurons. Science 241:1667-69
18. La Salle GL, Robert JJ, Berrard S, Ridoux V, Stratford-Perricaudet LD, et al. 1993. An adenovirus vector for gene transfer into neurons and glia in the brain. Science 259:988-90
19. Pettit DL, Perlman S, Malinow R. 1994. Potentiated transmission and prevention of further LTP by increased CaMKII activity in postsynaptic hippocampal slice neurons. Science 266:1881-85
20. Pettit DL, Koothan T, Liao D, Malinow R. 1995. Vaccinia virus transfection of hippocampal slice neurons. Neuron 14: 685-88
21. Ramsey-Ewing A, Moss B. 1995. Restriction of vaccinia virus replication in CHO cells occurs at the stage of viral intermediate protein synthesis. Virology 206.984-93
22. Lo DC, McAllister AK, Katz LC. 1994. Neuronal transfection in brain slices using particle-mediated gene transfer. Neuron 13:1263-68
23. Wu GY, Zou DJ, Koothan T, Cline HT. 1995. Infection of frog neurons with vaccinia virus permits in vivo expression of foreign proteins. Neuron 14:681-84
24. Elferink LA, Peterson MR, Scheller RH. 1993. A role for synaptotagmin (p65) in regulated exocytosis Cell 72:153-59
25. Hunt JM, Bommert K, Charlton MP, Kistner A, Habermann E, et al. 1994. A post-docking role for synaptobrevin in synaptic vesicle fusion Neuron 12: 1269-79
26. DeBello WM, O'Connor V, Dresbach T, Whiteheart SW, Wang SS-H, et al. 1995. SNAP-mediated protein-protein interactions essential for neurotransmitter release. Nature 373:626-30
27. Bommert K, Charlton MP, DeBello WM, Chin GJ, Betz H, Augustine GJ. 1993. Inhibition of neurotransmitter release by C2-domain peptides implicates synaptotagmin in exocytosis. Nature 363: 163-65
28. Richmond J, Haydon PG. 1993. Rab effector domain peptides stimulate the release of neurotransmitter from cell cultured synapses. FEBS Lett. 326:124-30
29. Oberhauser AF, Monck JR, Balch WE, Fernandez JM. 1992. Exocytotic fusion is activated by Rab3a peptides. Nature 360:270-73
30. Padfield PJ, Balch WE, Jamieson JD. 1992. A synthetic peptide of the Rab3a effector domain stimulates amylase release from permeabilized pancreatic acini. Proc. Natl. Acad. Sci. USA 89: 1656-60
31. MacLean CM, Law GJ, Edwardson JM. 1993. Stimulation of exocytotic membrane fusion by modified peptides of the rab3 effector domain; re-evaluation of the role of rab3 in regulated exocytosis. Biochem. J. 294:325-28
32. Alder J, Xie ZP, Valtorta F, Greengard P, Poo MM. 1992. Antibodies to synaptophysin interfere with transmitter secretion at neuromuscular synapses. Neuron 9:759-68
33. 2+ channel activity. Neuroscience 65: 905-15
34. Pieribone VA, Shupliakov O, Brodin L, Hilfiker-Rothenfluh S, Czernik AJ, Greengard P. 1995. Distinct pool of synaptic vesicles in neurotransmitter release Nature 375:493-97
35. Scheer H, Madeddu L, Dozio N, Gatti G, Vicentini LM, Meldolesi J. 1984. α-Latroxin of black widow spider venom: an interesting neurotoxin and a tool for investigating the process of neurotransmitter release. J. Physiol. 79: 216-21
36. Montecucco C, Schiavo G. 1994. Mechanism of action of tetanus and botulinum neurotoxins Mol Microbiol. 13:1-8
37. Hamill OP, Marty A, Neher E, Sakmann B, Sigworth FJ. 1981. Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches. Pfluegers Arch. 391:85-100
38. Augustine GJ, Neher E. 1992. Calcium requirements for secretion in bovine chromaffin cells. J. Physiol. 450:247-71
39. Pusch M, Neher E. 1988. Rates of diffusional exchange between small cells and a measuring patch pipette. Pfluegers Arch. 411:204-11
40. Morgan A, Burgoyne RD. 1992. Exol and Exo2 proteins stimulate calcium-dependent exocytosis in permeabilized adrenal chromaffin cells. Nature 355: 833-36
41. Hay JC, Martin TFJ. 1992. Resolution of regulated secretion into sequential MgATP-dependent and calcium-dependent stages mediated by distinct cytosolic proteins. J. Cell Biol. 119: 139-51
42. Bhakdi S, Weller U, Walev I, Martin E, Jonas D, Palmer M. 1993. A guide to the use of pore-forming toxins for controlled permeabilization of cell membranes. Med. Microbiol. Immunol. 182:167-75
43. Ahnert-Hilger G, Wegenhorst U, Stecher B, Spicher K, Rosenthal W, Gratz M. 1992. Exocytosis from permeabilized bovine adrenal chromaffin cells is differently modulated by guanosine 5′-[γ-thio]tripriosphate and guanosine 5′[γ-imido]triphosphate. Biochem. J. 284: 321-26
44. Schafer T, Karli UO, Gratwohl EK, Schweizer FE, Burger MM. 1987. Digitonin-permeabilized cells are exocytosis competent. J. Neurochem. 49:1697-707
45. Disatnik MH, Hernandez-Sotomayor SM, Jones G, Carpenter G, Mochly-Rosen D. 1994. Phospholipase C-gamma-1 binding to intracellular receptors for activated protein kinase C. Proc. Natl. Acad. Sci. USA 91:559-63
46. 2+-activated steps of dense core granuel exocytosis. Cold Spring Harbor Symp. Quant. Biol. In press
47. Martin TFJ. 1989. Cell cracking: permeabilizing cells to macromolecular probe. Methods Enzymol. 168:225-33
48. Rahamimoff R, Meiri H, Erulkar SD, Barenholz Y. 1978. Changes in transmitter release induced by ion-containing liposomes. Proc. Natl. Acad. Sci USA 75:5214-16
49. Heumann R, Schwab M, Thoenen H. 1981. A second messenger required for nerve growth factor biological activity? Nature 292:838-40
50. Tagaya M, Toyonaga S, Takahashi M, Yamamoto A, Fujiwara T, et al. 1995. Syntaxin 1 (HPC-1) Is associated with chromaffin granules. J. Biol. Chem. 270: 15930-33
51. Walch-Solimena C, Blasi J, Edelmann L, Chapman ER, von Mollard GF, Jahn R. 1995. The t-SNAREs syntaxin 1 and SNAP-25 are present on organelles that participate in synaptic vesicle recycling. J. Cell Biol. 128:637-45
52. Trimble WS, Cowan DM, Scheller RH. 1988. VAMP-1: a synaptic vesicle-associated integral membrane protein. Proc. Natl. Acad Sci. USA 85:4538-42
53. Baumert M, Maycox PR, Navone F, De Camilli P, Jahn R. 1989. Synaptobrevin: an integral membrane protein of 18,000 daltons present in small synaptic vesicles of rat brain. EMBO J. 8:379-84
54. Elferink LA, Trimble WS, Scheller RH. 1989. Two vesicle-associated membrane protein genes are differentially expressed in the rat central nervous system. J. Biol. Chem. 264:11061-64
55. Sudhof TC, Czernik AJ, Kao HT, Takei K, Johnston PA, et al. 1989. Synapsins: mosiacs of shared and individual domains in a family of synaptic vesicle phosphoproteins. Science 245:1474-80
56. McMahon HT, Ushkaryov YA, Edelmann L, Link E, Binz T, et al. 1993. Cellubrevin is a ubiquitous tetanus-toxin substrate homologous to a putative synaptic vesicle fusion protein. Nature 364: 346-49
57. Chilcote TJ, Galli T, Mundigl O, Edelmann L, McPherson PS, et al. 1995. Cellubrevin and synaptobrevins: similar subcellular localization and biochemical properties in PC12 cells. J. Cell Biol. 129:219-31
58. Sollner T, Whiteheart SW, Brunner M, Erdjument-Bromage H, Geromanos S, et al. 1993. SNAP receptors implicated in vesicle targeting and fusion. Nature 362:318-24
59. Pevsner J, Hsu S-C, Braun JEA, Calakos N, Ting AE, Bennett MK, et al. 1994. Specificity and regulation of a vesicle docking complex. Neuron 13: 353-61
60. Hayashi T, McMahon H, Yamasaki S, Binz T, Hata Y, et al. 1994. Synaptic vesicle membrane fusion complex: action of clostridial neurotoxins on assembly. EMBO J. 13:5051-61
61. Calakos N, Scheller RH 1994. Vesicle-associated membrane protein and synaptophysin are associated on the synaptic vesicle. J. Biol. Chem 269:24534-37
62. Washbourne P, Schiavo G, Montecucco C. 1995. Vesicle-associate membrane protein-2 (snaptobrevin-2) forms a complex with synaptophysin. Biochem J. 305:721-24
63. Edelmann L, Hanson PI, Chapman ER, Jahn R. 1995. Synaptobrevin binding to synaptophysin: a potential mechanism for controlling the exocytotic fusion machine. EMBO J. 14:224-31
64. Cornille F, Deloye F, Fournie-Zaluski M-C, Roques BP, Poulain B. 1995. Inhibition of neurotransmitter release by synthetic proline-rich peptides shows that the N-terminal domain of vesicle-associated membrane protein/synaptobrevin is critical for neuro-exocytosis. J. Biol. Chem. 270:16826-32
65. Schiavo G, Rossetto O, Montecucco C. 1994. Clostridial neurotoxins as tools to investigate the molecular events of neurotransmitter release. Semin. Cell Biol. 5:221-29
66. Pellegrini LL, O'Connor V, Betz H. 1994. Fusion complex formation protects synaptobrevin against proteolysis by tetanus toxin light chain. FEBS Lett. 353:319-23
67. Pellegrini LL, O'Connor V, Lottspeich F, Betz H. 1995. Clostridial neurotoxins compromise the stability of a low-energy SNARE complex mediating NSF activation of synaptic vesicle fusion. EMBO J. 14:4705-13
68. Li C, Ullrich B, Zhang JZ, Anderson RG, Brose N, et al. 1995. Ca-dependent and independent activation of neural and nonneural synaptotagmins. Nature 375: 594-99
69. Chapman ER, Jahn R. 1994. Calcium-dependent interaction of the cytoplasmic region of synaptotagmin with membranes. J. Biol. Chem. 269:5735-41
70. Brose N, Petrenko AG, Sudhof TC, Jahn R. 1992. Synaptotagmin: a calcium sensor on the synaptic vesicle surface. Science 256:1021-25
71. Ullrich B, Li C, Zhang JZ, McMahon H, Anderson RG, et al. 1994. Functional properties of multiple synaptotagmins in brain. Neuron 13:1281-91
72. 2+/phospholipid binding. J. Biol. Chem. 268: 26386-90
73. 2+/phospholipid-binding fold. Cell 80:929-38
74. + implications for membrane recycling. Cell 78.751-60
75. Hata Y, Davletov B, Petrenko AG, Jahn R, Sudhof TC. 1993. Interaction of synaptotagmin with the cytoplasmic domains of neurexins. Neuron 10:307-15
76. Perin MS. 1994 The COOH terminus of synapcotagmin mediates interaction with the neurexins. J. Biol. Chem. 269: 8576-81
77. Leveque C, El Far O, Martin-Moutot N, Sato K, Kato R, et al. 1994. Purification of the N-type calcium channel associated with syntaxin and synaptotagmin. J. Biol. Chem. 269:6306-12
78. 12+ channel complex. FEBS Lett. 326:255-60
79. Deleted in proof
80. Yoshida A, Oho C, Omori A, Kuwahara R, Ito T, et al. 1992. HPC-1 is associated with synaptotagmin and omega-conotoxin receptor. J. Biol. Chem. 267: 24925-28
81. 2+ channels. Science 263:981-82
82. Gundersen CB, Umbach JA. 1992. Suppression cloning of the cDNA encoding a candidate subunit of a presynaptic calcium channel. Neuron 9:527-37
83. Gundersen CB, Mastrogiacomo A, Faull K, Umbach JA. 1994. Extensive lipidation of a Torpedo cysteine string protein. J. Biol. Chem. 269:19197-99
84. Zinsmaier KE, Eberle KK, Buchner E, Walter N, Benzer S. 1994. Paralysis and early death of cysteine string protein mutants of Drosophila. Science 263: 977-80
85. Wiedenmann B, Franke WW. 1985. Identification and localization of synaptophysin, an integral membrane glycoprotein of Mr 38,000 characteristic of presynaptic vesicles. Cell 41:1017-28
86. Sudhof TC, Lottspeich F, Greengard P, Mehl E, Jahn R. 1987. A synaptic vesicle protein with a novel cytoplasmic domain and four transmembrane regions. Science 238:1142-44
87. Leube RE, Kaiser P, Seiter A, Zembelmann R, Franke WW, et al. 1987. Synaptophysin: molecular organization and mRNA expression as determined from cloned cDNA. EMBO J. 6:3261-68
88. Fykse EM, Takei K, Walch-Solimena C, Geppert M, Jahn R, et al. 1993. Relative properties and localizations of synaptic vesicle protein isoforms: the case of the synaptophysins. J. Neurosci. 13:4997-5007
89. Barnekow A, Jahn R, Schartl M. 1990. Synaptophysin: a substrate for the protein tyrosine kinase pp60C-src in intact synaptic vesicles. Oncogene 5:1019-24
90. Rubenstein JL, Greengard P, Czernik AJ. 1993. Calcium-dependent serine phosphorylation of synaptophysin. Synapse 13:161-72
91. Thomas L, Hartung K, Langosch D, Rehm H, Bamberg E, et al. 1988. Identification of synaptophysin as a hexameric channel protein of the synaptic vesicle membrane Science 242:1050-53
92. Thomas L, Beiz H. 1990. Synaptophysin binds to physophilin, a putative synaptic plasma membrane protein. J. Cell Biol. 111:2041-52
93. +-ATPase. J. Biol. Chem. 269: 28329-34
94. Knaus P, Marqueze-Pouey B, Scherer H, Betz H. 1990. Synaptoporin, a novel putative channel protein of synaptic vesicles. Neuron 5:453-62
95. Grabs D, Bergmann M, Schuster T, Fox PA, Brich M, Gratz M. 1994. Differential expression of synaptophysin and synaptoponn during pre- and postnatal development of the rat hippocampal network. Eur. J. Neurosci. 6:1765-71
96. Leube RE. 1994. Expression of the synaptophysin gene family is not restricted to neuronal and neuroendocrine differentiation in rat and human. Differentiation 56:163-71
97. Simons K, Zerial M. 1993. Rab proteins and the road maps for intracellular transport. Neuron 11:789-99
98. von Mollard GF, Stahl B, Khokhlatchev A, Sudhof TC, Jahn R. 1994. Rab3C is a synaptic vesicle protein that dissociates from synaptic vesicles after stimulation of exocytosis. J. Biol. Chem. 269: 10971-74
99. Johnson PA, Archer BT III, Robinson K, Mignery GA, Jahn R, et al 1991. Rab3a attachment to the synaptic vesicle membrane mediated by a conserved polyisoprenylated carboxy-terminal sequence. Neuron 7:101-9
100. Santos E, Nebreda AR 1989 Structural and functional properties of ras proteins FASEB J 3.2151-63
101. Arab S, Kikuchi A, Hata Y, Isomura M, Takai Y 1990 Regulation of reversible binding of smg p25A, J ras p21-ltke GTP-binding protein, to synaptic plasma membranes and vesicles by its specific regulatory protein, GDP dissociation inhibitor. J. Biol. Chem. 265:13007-15
102. Burstein ES, Macara IG. 1992. Characterization of a guanine nucleotide-releasing factor and a GTPase-activating protein that are specific for the ras-related protein p25 rab3A. Proc. Natl. Acad. Sci. USA 89:1154-58
103. Yamaguchi T, Shirataki H, Kishida S, Miyazaki M, Nishikawa J, et al. 1993. Two functionally different domains of Rabphilin-3A, Rab3A/smg p25A-binding and phospholipid and calcium-binding domains. J. Biol. Chem. 268: 27164-70
104. Shirataki H, Kaibuchi K, Sakoda T, Kishida S, Yamaguchi T, et al. 1993. Rabphilin-3A, a putative target protein for smg p25A/rab3A p25 small GTP-binding protein related to synaptotagmin. Mol. Cell Biol. 13:2061-68
105. 2+/phospholipid-binding protein, depends on rab3A/3C. Neuron 13:885-98
106. Shirataki H, Yamamoto T, Hagi S, Miura H, Oishi H, et al. 1995. Rabphilin-3A is associated with synaptic vesicles through a vesicle protein in a manner independent of Rab3A. J. Biol. Chem. 269:32717-20
107. Kishida S, Shirataki H, Sasaki T, Kato M, Kaibuchi K, et al. 1993. Rab3A GTPase-activating protein-inhibiting activity of Rabphilin-3A, a putative Rab3A target protein. J. Biol. Chem. 268:22259-61
108. Fujita Y, Sasaki T, Araki K, Takahashi K, Imazumi K, et al. 1994. GDP/GTP exchange reaction-stimulating activity of Rabphilin-3A for Rab3A small GTP-binding protein. FEBS Lett. 353:67-70
109. Miyazaki M, Shirataki H, Kohno H, Kaibuchi K, Tsugita A, et al. 1994. Identification as β-adducin of a protein interacting with Rabphifin-3A in the presence of calcium and phosphatidylserine. Biochem. Biophys. Res. Commun. 205:460-66
110. Orita S, Sasaki T, Naito A, Komuro R, Ohtsuka T, et al 1995. Doc2. a novel brain protein having two repeated C2-like domains. Biochem Biophys. Res. Commun. 206:439-48
111. 2+/calmodulin-dependent protein kinase. Adv. Second Messenger Phosphoprotein Res. 22:39-112
112. 2+/calmodulin-dependent protein kinase. Neuron 1:63-72
113. Hanks SK, Quinn AM, Hunter T. 1988. The protein kinase family: conserved features and deduced phylogeny of the catalytic domains. Science 241:42-52
114. Yamaguchi T, Ohsako S, Deguchi T. 1988. Expression and characterization of calmodulin-dependent protein kinase II from cloned cDNAs in Chinese hamster ovary cells. J. Biol. Chem. 264: 19108-16
115. Lin CR, Kapiloff MS, Durgerian S, Tatemoto K, Russo AF, et al. 1987. Molecular cloning of a brain-specific calcium-calmodulin-dependent protein kinase. Proc. Neat. Acad. Sci. USA 84: 5962-66
116. 2+/ calmoudlin-independent activity. Proc. Natl. Acad. Sci. USA 87:1273-77
117. 2+/calmodulin-dependent protein kinase II. J. Biol. Chem. 264:16759-63
118. 2+-independent activity of calmodulin-dependent protein kinase II alpha subunit. J. Biochem. 109:137-43
119. 2+/calmodulin-dependent protein kinase II is a binding protein for synapsin I. Nature 359:417-20
120. Ueda T, Maeno H, Greengard P. 1973. Regulation of endogenous phosphorylation of specific proteins in synaptic membrane fractions from rat brain by adenosine 3′5′-monophosphate. J. Biol. Chem. 248:8295-305
121. 2+/calmodulin-dependent protein kinase II. J. Biol. Chem. 260: 1696-704
122. DeCamilli P, Greengard P. 1986. Synapsin I: synaptic vesicle-associated phosphoprotein. Biochem. Pharmacol. 35:4349-57
123. Greengard P, Valtorta F, Czernik AJ, Benfenati F. 1993. Synaptic vesicle phosphoproteins and regulation of synaptic function. Science 259:780-85
124. Bahler M, Greengard P. 1987. Synapsin I bundles F-actin in a phosphorylation-dependent manner. Nature 326:704-7
125. McPherson PS, Takei K, Schmid SL, DeCamilli P. 1994. p145, a major Grb2-binding protein in brain, is co-localized with dynamin in nerve terminals where it undergoes activity dependent dephosphorylation. J. Biol. Chem. 269:30132-39
126. Petrucci TC, Morrow JS. 1987. Synapsin I: an actin-bundling protein under phosphorylation control. J. Cell Biol. 105:1355-63
127. Bennett MK, Garcia-Arraras JE, Elferink LA, Peterson K, Fleming AM, et al. 1993. The syntaxin family of vesicular transport receptors. Cell 74:863-73
128. Sollner T, Bennett MK, Whiteheart SW, Scheller RH, Rothman JE. 1993. A protein assembly-disassembly pathway in vitro that may correspond to sequential steps of synaptic vesicle docking, activation, and fusion. Cell 75:409-18
129. Bennett MK, Calakos N, Scheller RH. 1992. Syntaxin: a synaptic protein implicated in docking of synaptic vesicles at presynaptic active zones. Science 257: 255-59
130. Sheng ZH, Rettig J, Takahashi M, Catterall WA. 1994. Identification of a syntaxin-binding site on N-type calcium channels. Neuron 13:1303-13
131. Blasi J, Chapman ER, Yamasaki S, Binz T, Niemann H, Jahn R. 1993. Botulinum neurotoxin C1 blocks neurotransmitter release by means of cleaving HPC-1/syntaxin. EMBO J. 12:4821-28
132. Oyler GA, Higgins GA, Hart RA, Battenberg E, Billingsley M, et al. 1989. The identification of a novel synaptosomal-associated protein, SNAP-25, differentially expressed by neuronal subpopulations. J. Cell Biol. 109:3039-52
133. Hess DT, Slater TM, Wilson MC, Skene JHP. 1992. The 25 kDa synaptosomal-associated protein SNAP-25 is the major methionine-rich polypeptide in rapid axonal transport and a major substrate for palmitoylation in adult CNS. J. Neurosci. 12:4634-41
134. Chapman ER, An S, Barton N, Jahn R. 1994. SNAP-25, a t-SNARE which binds so both syntaxin and synaptobrevin via domains that may form coiled coils. J. Biol. Chem. 269:27427-32
135. Hayashi T, Yamasaki S, Nauenburg S, Binz T, Niemann H. 1995. Disassembly of the reconstituted synaptic vesicle membrane fusion complex in vitro. EMBO J. 14:2317-25
136. Blasi J, Chapman ER, Link E, Binz T, Yamasaki S, et al. 1993. Botulinum neurotoxin A selectively cleaves the synaptic protein SNAP-25. Nature 365: 160-63
137. Bark IC, Wilson MC. 1994. Human cDNA clones encoding two different isoforms of the nerve terminal protein SNAP-25. Gene 129:291-92
138. Rothman JE, Warren G. 1994. Implications of the SNARE hypothesis for intracellular membrane topology and dynamics. Curr. Biol. 4:220-33
139. Gengyo-Ando K, Kamiya Y, Yamakawa A, Kodaira KI, Nishiwaki K, et al. 1993. The C. elegans unc-18 gene encodes a protein expressed in motor neurons. Neuron 11:703-11
140. Hodel A, Schafer T, Gerosa D, Burger MM. 1994. In chromaffin cells, the mammalian Sec1p homologue is a syntaxin 1A-binding protein associated with chromaffin granules. J. Biol. Chem. 269:8623-26
141. Pevsner J, Hsu SC, Scheller RH. 1994. n-Sec1: a neural-specific syntaxin-binding protein. Proc. Natl. Acad. Sci. USA 91:1445-49
142. Hata Y, Sudhof TC. 1995. A novel ubiquitous form of munc-18 interacts with multiple syntaxins. J. Biol. Chem. 270:13022-28
143. Garcia EP, Gatti E, Butler M, Burton J, Decamilli P. 1994. A rat brain Sec1 homolog related to Rop and UNC18 interacts with syntaxin. Proc. Natl. Acad. Sci. USA 91:2003-7
144. Katagiri H, Terasaki J, Murata T, Ishihara H, Ogthara T, et al. 1995. A novel isoform of syntaxin-binding protein homologous to yeast sec1 expressed obiquitously in mammalian cells. J. BioL Chem. 270:4963-66
145. Tellan JT, McIntosh S, James DE. 1995. Molecular identification of two novel, munc-18 isoforms expressed in non-neuronal tissues. J Biol. Chem. 270:5857-63
146. Garcia EP, McPherson PS, Chilcote TJ, Takei K, DeCamilli P. 1995. rbSec1A and B colocalize with syntaxin 1 and SNAP-25 throughout the axon, but are not in a stable complex with syntaxin. J. Cell Biol. 129:105-20
147. Ushkaryov YA, Petrenko AG, Geppert M, Sudhof TC. 1992. Neurexins: synaptic cell surface proteins related to the alpha-latrotoxin receptor and laminin. Science 257:50-56
148. Ullrich B, Ushkaryov YA, Sudhof TC. 1995. Cartography of neurexins: more than 1000 isoforms generated by alternative splicing and expressed in distinct subsets of neurons. Neuron 14:497-507
149. Ichtchenko K, Hata Y, Nguyen T, Ullrich B, Missler M, et al. 1995. Neuroligin 1: a splice site-specific ligand for β-neurexins. Cell 81:435-43
150. Malhotra V, Orci L, Glick BS, Block MR, Rothman JE. 1988. Role of an N-ethylmaleimide-sensitive transport component in promoting fusion of transport vesicles with cisternae of the Golgi Stack. Cell 54:221-27
151. Deleted in proof
152. Wilson DW, Wilcox CA, Flynn GC, Chen E, Kuang WJ, et al. 1989. A fusion protein required for vesicle mediated transport in both mammalian cells and yeast. Nature 339:355-59
153. Hong RM, Mori H, Fukui T, Moriyama Y, Futai M, et al. 1994. Association of N-ethlmaleintide-sensetive factory with synaptic vesicles. FEBS Lett. 350:253-57
154. Whiteheart SW, Griff IC, Brunner M, Clary DO, Mayer T, et al. 1993. SNAP family of NSF attachment proteins includes a brain-specific isoform. Nature 362:353-55
155. Morgan A, Dimaline R, Burgoyne RD. 1994. The ATPase activity of N-ethyl-maleimide-sensitive fusion protein (NSF) is regulated by soluble SNF attachment proteins. J. Biol. Chem. 269:29347-50
156. Whiteheart SW, Kubalek EW. 1995. SNAPs and NSF: general members of the fusion apparatus. Trends Cell Biol. 5:64-68
157. Whiteheart SW, Rossnagel K, Buhrow SA, Brunner M, Jaenicke R, et al. 1994. N-ethylmaleimide-sensitive fusion protein: a trimeric ATPase whose hydrolysis of ATP is requried for membrane fusion. J. Cell Biol. 126:945-54
158. Pleasure IT, Black MM, Keen JH. 1993. Valosin-containing protein, VCP, is a ubiquitous clathrin-binding protein. Nature 365:459-62
159. Frohlich KU, Fries HW, Rudiger M, Erdmann R, Botstein D, Mecke D. 1991. Yeast cell cycle protein CDC48p shows full-length homology to the mammalian protein VCP and is a member of a protein family involved in secretion, peroxisome formation, and gene expression. J. Cell Biol. 114:443-53
160. McMahon HT, Sudhof TC. 1995. Synaptic core complex of synaptobrevin, suntaxin, and SNAP25 forms high affinity alpha-SNAP binding site. J. Biol. Chem. 270:2213-17
161. 2+-activated secretion. Nature 366:572-75
162. Hay JC, Fisette PL, Jenkins GH, Fukami K, Takenawa T, et al. 1995. ATP-dependent inositide phosphorylation required for Ca-activated secretion. Nature 374:173-77
163. Carpenter CL, Cantley LC. 1990. Phosphoinositide kinases. Biochemistry 29:11147-56
164. Jenkins GH, Fisette PL, Anderson RA. 1994. Type I phosphatidylinositol 4-phosphate 5-kinase isoforms are specifically stimulated by phosphatidic acid. J. Biol. Chem. 269:11547-54
165. 2+-regulated secretion in permeable neuroendocrine cells. Cell 70:765-75
166. Barroso M, Nelson DS, Sztul E. 1995. Transcytosis-associated protein (TAP)/-p115 is a general fusion factor required for binding of vesicles to acceptor membranes. Proc. Natl. Acad. Sci. USA 92:527-31
167. Sapperstein SK, Walter DM, Grosvenor AR, Heuser JE, Waters MG. 1995. p115 is a general vesicular transport factor related to the yeast endoplasmic reticulum to Golgi transport factor Usolp. Proc. Natl. Acad. Sci. USA 92:522-26
168. Morgan A, Burgoyne RD. 1992. Interaction between protein kinase C and Exol (14-3-3 protein) and its relevance to exocytosis in permeabilized adrenal chromaffin cells. Biochem. J. 286:807-11
169. Chamberlain LH, Roth G, Morgan A, Burgoyne RD. 1995. Distinct effects of α-SNAP, 14-3-3 proteins, and calmodulin on priming and triggering of regulated exocytosis. J. Cell Biol. 130:1063-70
170. Aitken A. 1995. 14-3-3 proteins on the map. Trends Biol. Sci. 20:95-97
171. 2+-dependent exocytosis in chromaffin cells. EMBO J. 12:3747-52
172. Vitale ML, Seward EP, Trifaro JM. 1995. Chromaffin cell cortical actin network dynamics control the size of the release-ready vesicle pool and the initial rate of exocytosis. Neuron 14:353-63
173. 2+ binding proteins and synaptic plasticity. Neurosci. Res 16:1-7
174. Mochida S, Kobayaski H, Matsuda Y, Yuda Y, Muramoto K, Nonomura Y. 1994. Myosin II is involved in transmitter release at synapses formed between rat sympathetic neurons in culture. Neuron 13:1131-42
175. Schweizer FE, Schafer T, Tapparelli C, Grob M, Karli UO, et al. 1989. Inhibition of exocytosis by intracellularly applied antibodies against a chromaffin granule-binding protein. Nature 339:709-12
176. Kelly RB. 1993. Storage and release of neurotransmitters. Cell 72:43-53
177. Kelly RB, Bonzelius F, Cleves A, Clift-O'Grady L, Grote E, Herman G, et al. 1993. Biogenesis of synaptic vesicles. J. Cell Sci. 17:81-83
178. Bennett MK, Scheller RH. 1994. A molecular description of synaptic vesicle membrane trafficking. Annu. Rev. Biochem. 63:63-100
179. Robinson MS. 1994. The role of clathrin, adaptors and dynamin in endocytosis. Curr. Opin. Cell Biol. 6:538-44
180. Betz WJ, Bewick GS. 1992. Optical analysts of synaptic vesicle recycling at the frog neuromuscuiar junction. Science 255:200-3
181. Ryan TA, Reuter H, Wendland B, Schweizer FE, Tsien RW, Smith SJ, et al. 1993. The kinetics of synaptic vesicle recycling measured at single presynaptic boutons. Neuron 11:713-24
182. Ryan TA, Smith SJ. 1995. Vesicle pool mobilization during action potential firing at hippocampal synapses. Neuron 14:983-89
183. (2+)-stimulated exocytosis in single pituitary cells. J. Cell Biol. 124:667-75
184. Parsons TD, Coorssen JR, Horstmann H, Almers W. 1995. Docked granules, the exocytic burst and the need for ATP hydrolysis in endocrine cells. Neuron 15:1085-96
185. Rothman JE. 1994. Mechanisms of intracellular protein transport. Nature 372:55-63
186. Warren G. 1993. Bridging the gap. Nature 362:297-98
187. Sudhof TC, De Camilli P, Niemann H, Jahn R. 1993. Membrane fusion machinery: insights from synaptic proteins. Cell 75:1-4
188. Schulze KL, Broadie K, Perin MS, Bellen HJ. 1995. Genetic and electrophysiological studies of Drosophila syntaxin-1A demonstrate its role in nonneuronal secretion and neurotransmission. Cell 80:311-20
189. Broadie K, Prokop A, Bellen HJ, O'Kane CJ, Schulze KJL, Sweeney ST, et al. 1995. Syntaxin and synaptobrevin function downstream of vesicle docking in Drosophila. Neuron 15:663-73
190. Harrison SD, Broadie K, van de Goor J, Rubin GM. 1994. Mutations in the Drosophila rop gene suggest a function in general secretion and synaptic transmission. Neuron 13:555-66
191. Schulze KL, Littleton JT, Salzberg A, Halachmi N, Stern M, et al. 1994. rop, a Drospohila homolog of yeast Sec1 and vertebrate n-Sec1/Munc-18 proteins, is a negative regulator of neurotransmitter release in vivo. Neuron 13:1099-108
192. Deleted in proof
193. Novick P, Brennwald P. 1993. Friends and family: the role of the Rab GTPases in vesicular traffic. Cell 75:597-601
194. Hess SD, Doroshenko PA, Augustine GJ. 1993. A functional role for GTP-binding proteins in synaptic synaptic vesicle cycling. Science 259:1169-72
195. Deleted in proof
196. Ngsee JK, Miller K, Wendland B, Scheller RH. 1990. Multiple GTP-binding proteins from cholinergic synaptic vesicles. J. Neurosci. 10:317-22
197. Fischer von Mollard G, Mignery GA, Baumert M, Perin MS, Hanson TJ, et al. 1990. Rab3 is a small GTP-binding protein exclusively localized to synaptic vesicles. Proc. Natl. Acad. Sci. USA 87:1988-92
198. Brennwald P, Kearns B, Champion K, Keranen S, Bankaitis V, Novick P, et al. 1994. Sec9 is a SNAP-25-like component of a yeast SNARE complex that may be the effector of Sec4 function in exocytosis. Cell 79:245-58
199. Sogaard M, Tani K, Ye RY, Geromanos S, Tempst P, et al. 1994. A Rab protein is required for the assembly of SNARE complexes in the docking of transport vesicles. Cell 78:937-48
200. Fischer von Mollard G, Sudhof TC, Jahn R. 1991. A small GTP-binding protein dissociates from synaptic vesicles during exocytosis. Nature 349.79-81
201. 2+-dependent exocytosis from adrenal chromaffin cells. J. Biol. Chem. 269: 10229-34
202. Johannes L, Lledo PM, Roa M, Vincent JD, Henry JP, et al. 1994. The GTPase Rab3a negatively controls calcium-dependent exocytosis in endocrine cells. EMBO J. 13:2029-37
203. Lledo PM, Vernier P, Vincent JD, Mason WT, Zorec R. 1993. Inhibition of Rab3B expression attenuates calcium-dependent exocytosis in rat anterior pituitary cells. Nature 364:540-44
204. Johannes L, Lledo PM, Roa M, Vincent JD, Henry JP, Darchen F. 1994 The GTPase Rab3a negatively controls calcium-dependent exocytosis in neuroendocrine cells. EMBO J. 13:2029-37
205. Plutner H, Schwaniger R, Pind S, Balch WE. 1990. Synthetic peptides of the Rab effector domain inhibit vesicular transport through the secretory pathway. EMBO J. 9:2375-83
206. Burton JL, Burns ME, Gatti E, Augustine GJ, DeCamilli P. 1994. Specific interactions of Mss4 with members of the Rab GTPase subfamily. EMBO J. 13:5547-58
207. 2+ sensor for transmitter release at a central synapse. Cell 79:717-27
208. Bittner MA, Holz RW. 1992. Kinetic analysis of secretion from permeabilized adrenal chromaffin cells reveals distinct components. J. Biol. Chem. 267:16219-25
209. Holz RW, Bittner MA, Peppers SC, Senter RA, Eberhard DA. 1989. MgATP-independent and MgATP-dependent exocytosis. J. Biol. Chem. 264:5412-19
210. Vogel SS, Chernomordik LV, Zimmerberg J. 1992. Calcium-triggered fusion of exocytotic granules requires proteins in only one membrane. J. Biol. Chem. 267:25640-43
211. Vogel SS, Leikina EA, Chernomordik LV. 1993. Lysophosphatidylcholine reversibly arrests exocytosis and viral fusion at a stage between triggering and membrane merger. J. Biol. Chem. 268:25764-68
212. Vilmart-Seuwen J, Kersken H, Sturzl R, Plattner H. 1986. ATP keeps exocytosis sites in a primed state but is not required for membrane fusion: an analysis with Paramecium cells in vivo and in vitro. J. Cell Biol. 103:1279-88
213. Kee Y, Lin RC, Hsu SC, Scheller RH. 1995. Distinct domains of syntaxin are required for synaptic vesicle fusion complex formation and dissociation. Neuron 14:991-98
214. O'Connor V, Augustine GJ, Betz H. 1994. Synaptic vesicle exocytosis: molecules and models. Cell 76:785-87
215. Morgan A, Burgoyne RD. 1995. A role for soluble NSF attachment proteins (SNAPs) in regulated exocytosis in adrenal chromaffin cells. EMBO J. 14:232-39
216. Pallanck L, Ordway RW, Ganetzky B. 1995. A Drosophila NSF mutant. Nature 376:25
217. Siddiqi O, Benzer S. 1976. Neurophysiological defects in temperature-sensitive paralytic mutants of Drosphila melano-gaster. Proc. Natl. Acad. Sci. USA 73:3253-57
218. Kumakura K, Sasaki K, Sakurai T, Ohara-Imaizumi M, Misonou H, et al. 1994. Essential role of myosin light chain kinase in the mechanism for MgATP-dependent priming of exocytosis in adrenal chromaffin cells. J. Neurosci. 14:7695-703
219. 2+ receptor controls the final steps in peptide secretion from pituitary melanotrophs. Neuron 11:93-104
220. Katz B, Miledi R. 1965. The effect of temperature on the synaptic delay at the neuromuscular junction. J. Physiol. 181:656-70
221. Katz B, Miledi R. 1967. A study of synaptic transmission in the absence of nerve impulses. J. Physiol. 192:407-36
222. Llinas R, Steinberg IZ, Walton K. 1981. Relationship between presynaptic calcium current and post synaptic potential in squid giant synapse. Biophys. J. 33:323-51
223. Augustine GJ, Charlton MP, Smith SJ. 1985. Calcium entry and neurotransmitter release at voltage-clamped nerve terminals of squid. J. Physiol. 367:163-81
224. Augustine GJ, Adler EM, Charlton MP. 1991. The calcium signal for transmitter secretion from presynaptic nerve teminals. Ann. NY Acad. Sci. 635:365-81
225. Adler EM, Augustine GJ, Duffy SN, Charlton MP. 1991. Alien intracellular calcium chelators attenuate neurotransmitter release at the squid giant synapse. J. Neurosci. 11:1496-507
226. Llinas R, Sugimori M, Silver RB. 1992. Microdomains of high calcium concentration in a presynaptic terminal. Science 256:677-79
227. Yamada WM, Zucker RS. 1992. Time course of transmitter release calculated from simulations of a calcium diffusion model. Biophys J. 61:671-82
228. Heidelberger R, Heinemann C, Neher E, Matthews G. 1994. Calcium dependence of the rate of exocytosis in a synaptic terminal. Nature 371:513-15
229. Dodge FA Jr, Rahamimoff R. 1967. Co-operative action of calcium ions in transmitter release at the neuromuscular junction. J. Physiol. 193:419-32
230. Augustine GJ, Charlton MP. 1986. Calcium-dependence of presynaptic calcium current and post-synaptic response at the squid giant synapse J. Physiol. 381:619-40
231. Augustine GJ, Eckert R. 1984. Divalent cations differentially support transmitter release at the squid giant synapse. J. Physiol. 346:257-71
232. Goda Y, Stevens CF. 1994. Two components of transmitter release at a central synapse. Proc. Natl. Acad. Sci. USA 91:12942-46
233. Zucker RS. 1989. Short-term synaptic plasticity. Annu. Rev. Neurosci. 12:13-31
234. Verhage M, McMahon HT, Ghijsen WE, Boomsma F, Scholten G, et al. 1991. Differential release of amino acids, neuropeptides, and catecholamines from isolated nerve terminals. Neuron 6:517-24
235. 2+ elevation above a threshold level in bull-frog sympathetic ganglia. Neuron 10:465-73
236. Burns ME, Augustine GJ. 1995. Synaptic structure and function: dynamic organization yields architectural precision. Cell 83:187-94
237. Chow RH, vonRuden L, Neher E. 1992. Delay in vesicle fusion revealed by electrochemical monitoring of single secretory events in adrenal chromaffin cells. Nature 356:60-63
238. Horrigan FT, Bookman RJ. 1994. Releasable pools and the kinetics of exocytosis in adrenal chromaffin cells. Neuron 13:1119-29
239. Heinemann C, Chow RH, Neher E, Zucker RS. 1994. Kinetics of the secretory response in bovine chromaffin cells following flash photolysis of caged calcium. Biophys. J. 67:2546-57
240. Chow RH, Klingauf J, Neher E. 1994. Time course of Ca concentration triggering exocytosis in neuroendocrine cells. Proc. Natl. Acad. Sci. USA 91:12765-69
241. Robinson EM, Finnegan JM, Monck JR, Wightman RM, Fernandez JM. 1995. Colocalization of calcium entry and exocytotic release sites in adrenal chromaffin cells. Proc. Natl. Acad. Sci. USA 92:2474-78
242. Neher E, Zucker RS. 1993. Multiple calcium-dependent processes related to secretion in bovine chromaffin cells. Neuron 10:21-30
243. Popov SV, Poo MM. 1993. Synaptotagmin: a calcium-sensitive inhibitor of exocytosis? Cell 73:1247-49
244. DeBello WM, Betz H, Augustine GJ. 1993. Synaptotagmin and neurotransmitter release. Cell 74:947-50
245. Littleton JT, Bellen HJ. 1995. Synaptotagmin controls and modulates synaptic vesicle fusion machinery in a calcium dependent manner. Trends Neurosci. 18:177-83
246. Broadie K, Bellen HJ, DiAntonio A, Littleton JT, Schwarz TL. 1994. Absence of synaptotagmin disrupts excitation-secretion coupling during synaptic transmission. Proc. Natl. Acad. Sci. USA 91:10727-31
247. Littleton JT, Stern M, Perin M, Bellen HJ. 1994. Calcium dependence of neurotransmitter release and rate of spontaneous vesicle fusions are altered in Drosophila synaptotagmin mutants. Proc. Natl. Acad. Sci. USA 91:10888-92
248. Shoji-Kasai Y, Yoshida A, Sato K, Hoshino T, Ogura A, et al. 1992. Neurotransmitter release from synaptotagmin-deficient clonal variants of PC12 cells. Science 256:1820-23
249. Jorgensen EM, Hartwieg E, Schuske K, Nonet ML, Jin Y, Horvitz HR. 1995. Defective recycling of synaptic vesicles in synaptotagmin mutants of Caenorhabditis elegans. Nature 378:196-99
250. Chung S-H, Takai Y, Holz RW. 1995. Evidence that the Rab3a-binding protein, Rabphilin3a, enhances regulated secretion J. Biol. Chem. 270:16714-18
251. Burns ME, Fukui D, Sasaki Y, Takai Y, Augustine GJ. 1995. Rabphilin3A regulates neurotransmitter release at the squid giant synapse. Soc. Neurosci. Abstr. 21:326
252. White JM. 1992. Membrane fusion. Science 258:917-24
253. Zimmerberg J, Vogel SS, Chernomordik LV. 1993. Mechanisms of membrane fusion. Annu. Rev. Biophys. Biomol. Struct. 22:433-66
254. Chernomordik L, Chanturiya A, Green J. Zimmerberg J. 1995. The hemifusion intermediate and its conversion to complete fusion: regulation by membrane composition. Biophys. J. 69: 922-29
255. Kemble GW, Darueli T, White JM. 1994. Lipid-anchored influenza hemagglutinin promotes hemifusion, not complete fusion. Cell 76:383-91
256. Palade G 1975 Intracellular aspects of the process of protein synthesis. Science 189:347-58
257. Ornberg RL, Reese TS. 1981 Beginning of exocytosis captured by rapid-frezing of Limulus amebocytes. J. Cell Biol. 90:40-54
258. Almers W, Tse FW. 1990. Transmitter release from synapses: Does a preassembled fusion pore initiate exocytosis? Neuron 4:813-18
259. Monck JR, Fernandez JM. 1994. The exocytotic fusion pore and neurotransmitter release. Neuron 12:707-16
260. Alvarez de Toledo G, Fernandez-Chacon R, Fernandez JM. 1993. Release of secretory products during transient vesicle fusion. Nature 363:554-58
261. Alder J, Kanki H, Valtorta F, Greengard P, Poo MM. 1995. Overexpression of synaptophysin enhances neurotransmitter secretion at Xenopus neuromuscular synapses. J. Neurosci. 15:511-19
262. 2+-activated channel in neuorohypophysial granules. Neuron 8:335-42
263. Deleted in proof
264. Gundersen CB, Mastrogiacomo A, Umbach JA. 1995. Cysteine-string proteins as templates for membrane fusion: models of synaptic vesicle exocytosis. J. Theor. Biol. 172:269-77
265. Umbach JA, Zinsmaier KE, Eberle KK, Buchner E, Benzer S, et al. 1994. Presynaptic dysfunction in Drosophila csp mutants. Neuron 13:899-907
266. 2+-dependent conformational change in synaptotagmin I. J. Biol. Chem. 269: 28547-50
267. Otto H, Hanson PI, Chapman ER, Blasi J, Jahn R. 1995. Poisoning by botulinum neurotoxin A does not inhibit formation or disassembly of the synaptosomal fusion complex. Biochem. Biophys. Res. Commun. 212:945-52
268. Hanson PI, Otto H, Barton N, Jahn R. 1995. The N-ethylmaleimide-sensitive fusion protein and α-SNAP induce a conformational change in syntaxin. J. Biol. Chem. 270:16955-61
269. Fukuda M, Aruga J, Niinobe M, Aimoto S, Mikoshiba K. 1994. Inositol-1,3,4,5-tetrakisphosphate binding to C2B domain of IP4BP/synaptotagmin II. J. Biol. Chem. 269:29206-11
270. Ye W, Ali N, Bembenek ME, Shears SB, Lafer EM. 1995. Inhibition of clathrin assembly by high affinity binding of specific inositol polyphosphates J. Biol. Chem. 270:1564-68
271. Llinas R, Sugimori M, Lang EJ, Monta M, Fukuda M, et al. 1994. The inositol high-polyphosphate series blocks synaptic transmission by preventing vesicular fusion: a squid giant synapse study. Proc. Natl. Acad. Sci. USA 91:12990-93
272. Mikoshiba K, Fukuda M, Moreira JE, Lewis FMT, Sugimori M, Niiobe M, Llinas R. 1995. Role of the C2A domain of synaptotagmin in transmitter release as determined by specific antibody injection into the squid giant synapse preterminal. Proc. Natl. Acad. Sci. USA 92:10703-7
273. Muallem S, Kwiatkowska K, Xu X, Yin HL. 1995. Actin filament disassembly is a sufficient final trigger for exocytosis in nonexcitable cells. J. Cell Biol. 128:589-98