The rat norepinephrine transporter: Molecular cloning from PC12 cells and functional expression

Molecular Brain Research

Volumn 52, Issue 2, 1997, Pages 257-262

  Brüss, Michael ^a   Pörzgen, Peter ^a,b   Bryan Lluka, Lesley J ^b   Bönisch, Heinz ^a  

^a UNIVERSITY OF BONN   (Germany)

^b UNIVERSITY OF QUEENSLAND   (Australia)

Author keywords

Casein kinase II; Cloning; Glycosylation; Norepinephrine transporter; PC12 cell; Protein kinase C; Rat; Transfection

Indexed keywords

CARRIER PROTEIN; CHLORIDE; COMPLEMENTARY DNA; DESIPRAMINE; DOPAMINE TRANSPORTER; NEUROTRANSMITTER; NISOXETINE; NORADRENALIN; PROTEIN KINASE C; SODIUM; TRITIUM;

AMINO ACID SEQUENCE; ANIMAL CELL; ARTICLE; CATTLE; CONTROLLED STUDY; HUMAN; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PHEOCHROMOCYTOMA CELL; PRIORITY JOURNAL; PROTEIN GLYCOSYLATION; RAT; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

ADRENAL GLAND NEOPLASMS; ALGORITHMS; AMINO ACID SEQUENCE; ANIMALS; CARRIER PROTEINS; CASEIN KINASE II; CATTLE; CLONING, MOLECULAR; GLYCOSYLATION; HUMANS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; NOREPINEPHRINE; NOREPINEPHRINE PLASMA MEMBRANE TRANSPORT PROTEINS; PC12 CELLS; PHEOCHROMOCYTOMA; PHOSPHORYLATION; PROTEIN KINASE C; PROTEIN STRUCTURE, SECONDARY; PROTEIN-SERINE-THREONINE KINASES; RATS; RECOMBINANT PROTEINS; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY, AMINO ACID; SYMPORTERS; TRANSFECTION;

ANIMALIA; BOS TAURUS; BOVINAE; TRITIUM;

EID: 0031574095     PISSN: 0169328X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0169-328X(97)00267-2     Document Type: Article

References (32)

1. [1] S.G. Amara, M.J. Kuhar, Neurotransmitter transporters: recent progress, Annu. Rev. Neurosci. 16 (1993) 73-93.
2. [2] H. Bönisch, M. Brüss, The noradrenaline transporter of the neuronal plasma membrane, Ann. NY Acad. Sci. 733 (1994) 193-202.
3. 3H-desipramine to the neuronal noradrenaline carrier of rat phaeochromocytoma cells (PC12 cells), Naunyn-Schmiedeberg's Arch. Pharmacol. 334 (1986) 403-411.
4. [4] M. Brüss, R. Hammermann, S. Brimijoin, H. Bönisch, Antipeptide antibodies confirm the topology of the human norepinephrine transporter, J. Biol. Chem. 270 (1995) 9197-9201.
5. [5] C. Chen, H. Okayama, High efficiency transformation of mammalian cells by plasmid DNA, Cell. Biol. 7 (1987) 2745-2752.
6. [6] P. Chomczynski, N. Sacchi, Single step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction, Anal. Biochem. 162 (1987) 156-159.
7. [7] J.F. Cubells, K.S. Kim, H. Baker, B.T. Volpe, Y. Chung, T.A. Houpt, T.C. Wessel, T.H. Job, Differential in vivo regulation of mRNA encoding the norepinephrine transporter and tyrosine hydroxylase in rat adrenal medulla and locus ceruleus, J. Neurochem. 65 (1995) 502-509.
8. [8] C.D. Dunigan, A.E. Shamoo, Identification of the major transport pathway for the Parkinson-inducing neurotoxin 1-methyl-4-phenyl-pyridinium, Neuroscience 75 (1996) 37-41.
9. [9] A. Eshleman, C. Dunigan, A. Shamoo, M. Eldefrawi, ATP enhances catecholamine uptake into PC12 cells, Life Sci. 56 (1995) 1613-1621.
10. - in transport, Naunyn-Schmiedeberg's Arch. Pharmacol. 333 (1986) 246-252.
11. [11] M.A. Frohman, M.K. Dush, G.R. Martin, Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer, Proc. Natl. Acad. Sci. USA 85 (1988) 8998-9002.
12. [12] B. Giros, S. El Mestikawy, N. Goginat, K. Zheng, H. Han, T. Yang-Feng, M.G. Caron, Cloning, pharmacological characterization, and chromosome assignement of the human dopamine transporter, Mol. Pharmacol. 42 (1992) 383-390.
13. [13] K.-H. Graefe, H. Bönisch, The transport of amines across the axonal membranes of noradrenergic and dopaminergic neurones, in: U. Trendelenburg, N. Weiner (Eds.), Handbook of Experimental Pharmacology: Catecholamines I, Vol. 90/I, Springer, Berlin, 1988, pp. 247-278.
14. [14] L.A. Green, G. Rein, Release, storage and uptake of catecholamines by a clonal cell line of nerve growth factor (NGF) responsive pheochromocytoma cells, Brain Res. 129 (1977) 247-263.
15. [15] R. Hammermann, B. Lingen, M. Brüss, H. Bönisch, Regulation of the sodium-dependent noradrenaline transporter (NAT) by protein kinase C, Biol. Chem. 377 (Suppl.) (1996) S26.
16. [16] R. Harder, H. Bönisch, Effect of monovalent ions on the transport of noradrenaline across the plasma membrane of neuronal cells (PC12 cells), J. Neurochem. 45 (1985) 1154-1162.
17. [17] T. Kadota, T. Yamaai, Y. Saito, Y. Akita, S. Kawashima, K. Moroi, N. Inagaki, K. Kadota, Expression of dopamine transporter at the tips of growing neurites of PC12 cells, J. Histochem. Cytochem. 44 (1996) 989-996.
18. [18] J.E. Kilty, D. Lorang, S.G. Amara, Cloning and expression of a cocaine-sensitive rat dopamine transporter, Science 254 (1991) 578-579.
19. [19] P. Klein, M. Kanehisa, C. DeLisi, The detection and classification of membrane-spanning proteins, Biochim. Biophys. Acta 815 (1985) 468-476.
20. [20] M. Koide, A.K. Cho, B.D. Howard, Characterization of xylamine binding to proteins of PC12 pheochromocytoma cells, J. Neurochem. 47 (1986) 1277-1285.
21. [21] M. Kozak, Structural features in eukaryotic mRNAs that modulate the initiation of translation, J. Biol. Chem. 266 (1991) 19867-19870.
22. [22] D.E. Krantz, D. Peter, Y.J. Liu, R.H. Edwards, Phosphorylation of a vesicular monoamine transporter by casein kinase II, J. Biol. Chem. 272 (1997) 6752-6759.
23. [23] J. Kyte, R.F. Doolittle, A simple method for displaying the hydropathic character of a protein, J. Mol. Biol. 157 (1982) 105-132.
24. [24] B. Lingen, M. Brüss, H. Bönisch, Cloning and expression of the bovine sodium-and chloride-dependent noradrenaline transporter, FEBS Lett. 342 (1994) 235-238.
25. [25] D. Lorang, S.G. Amara, R.B. Simerly, Cell-type-specific expression of catecholamine transporters in the rat brain, J. Neurosci. 14 (1994) 4903-4914.
26. [26] H.E. Melikian, S. Ramamoorthy, G.G. Tate, R.D. Blakely, Inability to N-glycosylate the human norepinephrine transporter reduces protein stability, surface trafficking, and transport activity but not ligand recognition, Mol. Pharmacol. 50 (1996) 266-276.
27. [27] T.T. Nguyen, S.G. Amara, N-linked oligosaccharides are required for cell surface expression of the norepinephrine transporter but do not influence substrate or inhibitor recognition, J. Neurochem. 67 (1996) 645-655.
28. [28] T. Pacholczyk, R.D. Blakely, S.G. Amara, Expression cloning of a cocaine-and antidepressant-sensitive human noradrenaline transporter, Nature 350 (1991) 350-354.
29. [29] B. Ramachandran, K. Houben, Y.Y. Rozenberg, J.R. Haigh, A. Varpetian, B.D. Howard, Differential expression of transporters for norepinephrine and glutamate in wild type, variant, and Wnt1-expressing PC12 cells, J. Biol. Chem. 268 (1993) 23891-23897.
30. [30] F. Sanger, S. Nicklen, A.R. Coulson, DNA sequencing with chain terminating inhibitors, Proc. Natl. Acad. Sci. USA 74 (1977) 5463-5467.
31. [31] A.R. Wakade, T.D. Wakade, M. Poosch, M.J. Bannon, Noradrenaline transport and transporter mRNA of rat chromaffin cells are controlled by dexamethasone and nerve growth factor, J. Physiol. 494 (1996) 67-75.
32. [32] J. Zhu, T.D. Hexum, Characterization of cocaine-sensitive dopamine uptake in PC12 cells, Neurochem. Int. 21 (1992) 521-526.