Phospholipase D-catalyzed transphosphatidylation in anhydrous organic solvents

Biotechnology and Bioengineering

Volumn 72, Issue 3, 2001, Pages 374-377

  Rich, Joseph O ^a   Khmelnitsky, Yuri L ^a  

^a EnzyMed Division   (United States)

Author keywords

Enzymes in organic solvents; Phospholipase D; Transphosphatidylation

Indexed keywords

ALCOHOLS; AROMATIC HYDROCARBONS; CATALYSIS; CATALYST ACTIVITY; HYDROLYSIS; ION EXCHANGE RESINS; ORGANIC SOLVENTS;

PHOSPHOLIPASE; TRANSPHOSPHATIDYLATION;

ENZYMES;

ALCOHOL; CHOLINE; CHOLINE DERIVATIVE; ORGANIC SOLVENT; PHOSPHATIDYLCHOLINE; PHOSPHOLIPASE D; SCAVENGER; SODIUM CHLORIDE;

ARTICLE; CATALYSIS; CATION EXCHANGE; CHEMICAL REACTION; CONTROLLED STUDY; EXPERIMENTAL MODEL; HYDROLYSIS; NONHUMAN; REACTION ANALYSIS; STREPTOMYCES;

1,2-DIPALMITOYLPHOSPHATIDYLCHOLINE; ALCOHOLS; ANION EXCHANGE RESINS; CHLOROFORM; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; MOLECULAR STRUCTURE; PHOSPHOLIPASE D; RESINS, SYNTHETIC; SOLVENTS; STREPTOMYCES;

STREPTOMYCES SP.;

EID: 0035809096     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/1097-0290(20010205)72:3<374::AID-BIT16>3.0.CO;2-E     Document Type: Article

References (17)

1. Testing for diffusion limitations in salt-activated enzyme catalysts operating in organic solvents
2. On the pH memory of lyophilized compounds containing protein functional groups
3. Preparative transformation of natural phospholipids catalyzed by phospholipase D from Streptomyces
4. The evolution of biotransformation technologies
5. Can conformational changes be responsible for solvent and excipient effects on the catalytic behavior of subtilisin Carlsberg in organic solvents?
6. Nucleosides and nucleotides. 150. Enzymic synthesis of 5′-phosphatidyl derivatives of 1-(2-C-cyano-2-deoxy-beta-D-arabino-pentofuranosyl) cytosine (CNDAC) and their notable antitumor effects in mice
7. Repeated batch and continuous operations for phosphatidylglycerol synthesis from phosphatidylcholine with immobilized phospholipase D
8. Increasing productivity by removing choline in conversion of phosphatidylcholine to phosphatidylserine by phospholipase D
9. Synthesis of water-soluble paclitaxel derivatives by enzymatic acylation
10. Biocatalysis in nonaqueous solvents
11. Salts dramatically enhance activity of enzymes suspended in organic solvents
12. Why are enzymes less active in organic solvents than in water?
13. Simple transphosphatidylation of phospholipids catalysed by a lipid-coated phospholipase D in organic solvents
14. Optimizing the salt-induced activation of enzymes in organic solvents: Effects of lyophilization time and water content
15. Nucleosides and nucleotides. 150. Enzymic synthesis of 5′-phosphatidyl derivatives of 1-(2-C-cyano-2-deoxy-beta-D-arabino-pentofuranosyl) cytosine (CNDAC) and their notable antitumor effects in mice
16. Transphosphatidylation reaction of phosphatidylcholine to 4-methoxyphenol in water-immiscible organic solvents with immobilized phospholipase D
17. The effect of water on enzyme action in organic media