Design and Properties of NCCG-gp41, a Chimeric gp41 Molecule with Nanomolar HIV Fusion Inhibitory Activity

Journal of Biological Chemistry

Volumn 276, Issue 31, 2001, Pages 29485-29489

  Louis, John M ^b   Bewley, Carole A ^a   Clore, G Marius ^a  

^a NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES   (United States)

^b NONE

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBODIES; BIOASSAY; CELLS; ENZYME INHIBITION; IMMUNOLOGY; THERMODYNAMIC STABILITY; VIRUSES;

CELL FUSION; FUSOGENIC FORMS;

BIOCHEMISTRY;

CHIMERIC PROTEIN; CYSTEINE; GLYCINE; GLYCOPROTEIN GP 41; HUMAN IMMUNODEFICIENCY VIRUS VACCINE; NCCG GP41 PROTEIN; UNCLASSIFIED DRUG; VIRUS ENVELOPE PROTEIN; ANTI HUMAN IMMUNODEFICIENCY VIRUS AGENT; CD4 ANTIGEN; DISULFIDE; HYBRID PROTEIN;

AMINO TERMINAL SEQUENCE; ANIMAL CELL; ARTICLE; ASSAY; CELL FUSION; CHIMERA; DISULFIDE BOND; HUMAN IMMUNODEFICIENCY VIRUS; HUMAN IMMUNODEFICIENCY VIRUS INFECTION; IC 50; MOUSE; NONHUMAN; PRIORITY JOURNAL; VIRUS ENVELOPE; AMINO ACID SEQUENCE; ANIMAL; BIOSYNTHESIS; CELL LINE; CELL STRAIN 3T3; CHEMICAL STRUCTURE; CHEMISTRY; CIRCULAR DICHROISM; DRUG DESIGN; DRUG EFFECT; GENETICS; HUMAN; HUMAN IMMUNODEFICIENCY VIRUS 1; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PHYSIOLOGY; PROTEIN CONFORMATION; PROTEIN ENGINEERING; PROTEIN FOLDING; PROTEIN SECONDARY STRUCTURE; ULTRAVIOLET SPECTROPHOTOMETRY; X RAY CRYSTALLOGRAPHY;

ANIMALIA; HUMAN IMMUNODEFICIENCY VIRUS; RNA VIRUSES;

3T3 CELLS; AIDS VACCINES; AMINO ACID SEQUENCE; ANIMALS; ANTI-HIV AGENTS; ANTIGENS, CD4; BASE SEQUENCE; CELL FUSION; CELL LINE; CIRCULAR DICHROISM; CRYSTALLOGRAPHY, X-RAY; DISULFIDES; DRUG DESIGN; HIV ENVELOPE PROTEIN GP41; HIV-1; HUMANS; MICE; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; PROTEIN CONFORMATION; PROTEIN ENGINEERING; PROTEIN FOLDING; PROTEIN STRUCTURE, SECONDARY; RECOMBINANT FUSION PROTEINS; SPECTROPHOTOMETRY, ULTRAVIOLET; VIRAL ENVELOPE PROTEINS;

EID: 0035800816     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.C100317200     Document Type: Article

References (34)

1. Freed, E. O., and Martin, M. A. (1995) J. Biol. Chem. 270, 23883-23886
2. Moore, J. P., Trkola, A., and Dragic, T. (1997) Curr. Opin. Immunol. 9, 551-562
3. McMichael, A. J., and Hanke, T. (1999) Nat. Med. 5, 612-614
4. Kwong, P. D., Wyatt, R., Robinson, J., Sweet, R. W., Sodroski, J., and Hendrickson, W. A. (1998) Nature 393, 648-659
5. Boyd, M. R., Gustafson, K. R., McMahon, J. B., Shoemaker, R. H., O'Keefe, B. R., Mori, T., Gulakowski, R. J., Wu, L., Rivera, M. I., Laurencot, C. M., Currens, M. J., Cardellina, J. H., Buckheit, R. W., Nara, P. L., Pannell, L. K., Sowder, R. C., and Henderson, L. E. (1997) Antimicrob. Agents Chemother. 41, 1521-1530
6. Bewley, C. A., and Otero-Quintero, S. (2001) J. Am. Chem. Soc. 123, 3892-3902
7. Chan, D. C., and Kim, P. S. (1998) Cell 93, 681-684
8. Caffrey, M., Cai, M., Kaufman, J., Stahl, S. J., Wingfield, P. T., Covell, D. G., Gronenborn, A. M., and Clore, G. M. (1998) EMBO J. 17, 4572-4584
9. Chan, D. C., Fass, D., Berger, J. M., and Kim, P. S. (1997) Cell 89, 263-273
10. Weissenhorn, W., Dessen, A., Harrison, S. C., Skehel, J. J., and Wiley, D. C. (1997) Nature 387, 426-430
11. Tan, K. J., Liu, J., Wang, S., Shen, S., and Lu, M. (1997) Proc. Natl. Acad. Sci. U. S. A. 94, 12303-12308
12. Malashkevich, V. N., Chan, D. C., Chutkowski, C. T., and Kim, P. S. (1998) Proc. Natl. Acad. Sci. U. S. A. 95, 9134-9139
13. Wild, C. T., Oas, T., McDanal, C. B., Bolognesi, D., and Matthews, T. J. (1992) Proc. Natl. Acad. Sci. U. S. A. 89, 10537-10541
14. Wild, C. T., Shugars, D. C., Greenwell, T. K., McDanal, C. B., and Matthews, T. J. (1994) Proc. Natl. Acad. Sci. U. S. A. 91, 9770-9774
15. Lu, M., Blacklow, S. C., and Kim, P. S. (1995) Nat. Struct. Biol. 2, 1075-1082
16. Chan, D. C., Chutkowski, C. T., and Kim, P. S. (1998) Proc. Natl. Acad. Sci. U. S. A. 95, 15613-15617
17. Kilby, J. M., Hopkins, S., Venetta, T. M., DiMassimo, B., Cloud, G. A., Lee, J. Y., Alldredge, L., Hunter, E., Lambert, D., Bolognesi, D., Matthews, T., Johnson, M. R., Nowak, M. A., Shaw, G. M., and Saag, M. S. (1998) Nat. Med. 4, 1302-1307
18. Furuta, R. A., Wild, C. T., Weng, Y., and Weiss, C. D. (1998) Nat. Struct. Biol. 5, 276-279
19. Weng, Y., and Weiss, C. D. (1998) J. Virol. 72, 9676-9682
20. Eckert, D. M., Malashkevich, V. N., Hong, L. H., Carr, P. A., and Kim, P. S. (1999) Cell 99, 103-115
21. Ferrer, M., Kapoor, T. M., Strassmaier, T., Weissenhorn, W., Skehel, J. J., Oprian, D., Schreiber, S. L., Wiley, D. C., and Harrison, S. C. (1999) Nat. Struct. Biol. 6, 953-960
22. Louis, J. M., Wondrak, E. M., Copeland, T. D., Smith, C. A. D., Mora, P. T., and Oroszlan, S. (1989) Biochem. Biophys. Res. Comm. 159, 87-94
23. Andrade., M. A., Chacon, P., Merelo, J. J., and Moran, F. (1993) Protein Eng. 6, 383-390
24. Perkins, S. J. (1986) Eur. J. Biochem. 157, 169-180
25. Broder, C. C., and Berger, E. (1995) Proc. Natl. Acad. Sci. U. S. A. 92, 9004-9008
26. Feng, Y., Broder, C. C., Kennedy, P. E., and Berger, E. A. (1996) Science 272, 872-877
27. Alexander, W. A., Moss, B., and Fuerst, T. T. (1992) J. Virol. 66, 2934-2942
28. Salzwedel, K., Smith, E., Dey, B., and Berger, E. J. (2000) J. Virol. 74, 326-333
29. Brünger, A. T. (1993) X-PLOR: A System for X-ray Crystallography and NMR, Yale University Press, New Haven, CT
30. Schwieters, C. D., and Clore, G. M. (2001) J. Magn. Reson. 149, 239-244
31. Farzan, M., Choe, H., Desjardins, E., Sun, Y., Kuhn, J., Cao, J., Archambault, D., Kolchinski, P., Koch, M., Wyatt, R., and Sodroski, J. (1998) J. Virol. 72, 7620-7625
32. Yang, F., Bewley, C. A., Louis, J. M., Gustafson, K. R., Boyd, M. R., Gronenborn, A. M., Clore, G. M., and Wlodawer, A. (1999) J. Mol. Biol. 288, 403-412
33. Caffrey, M., Cai, M., Kaufman, J., Stahl, S. J., Wingfield, P. T., Gronenborn, A. M., and Clore, G. M. (1997) J. Mol. Biol. 271, 819-826
34. Root, M. J., Kay, M. S., and Kim, P. S. (2001) Science 291, 884-888