Systematic mutagenesis of the Helicobacter pylori cag pathogenicity island: Essential genes for CagA translocation in host cells and induction of interleukin-8

Molecular Microbiology

Volumn 42, Issue 5, 2001, Pages 1337-1348

  Fischer, Wolfgang ^a   Püls, Jürgen ^a   Buhrdorf, Renate ^a   Gebert, Bettina ^a   Odenbreit, Stefan ^a   Haas, Rainer ^a  

^a UNIVERSITY OF MUNICH   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CAGA PROTEIN; INTERLEUKIN 8; POLYPEPTIDE;

ARTICLE; BACTERIAL MUTATION; BACTERIAL STRAIN; BACTERIUM ISOLATION; CYTOKINE PRODUCTION; CYTOKINE RELEASE; EUKARYOTIC CELL; GENE DELETION; GENE EXPRESSION; GENE FUNCTION; GENE IDENTIFICATION; GENE TRANSLOCATION; HELICOBACTER PYLORI; HOST CELL; NONHUMAN; OPERON; POINT MUTATION; PRIORITY JOURNAL; PROTEIN INDUCTION; RHIZOBIUM RADIOBACTER; RNA TRANSLATION; SEQUENCE HOMOLOGY;

AMINO ACID SEQUENCE; ANTIGENS, BACTERIAL; BACTERIAL PROTEINS; BASE SEQUENCE; CLONING, MOLECULAR; DNA TRANSPOSABLE ELEMENTS; EPITHELIAL CELLS; GENE DELETION; HELICOBACTER PYLORI; HUMANS; INTERLEUKIN-8; MUTAGENESIS, INSERTIONAL; RECOMBINANT FUSION PROTEINS; RESTRICTION MAPPING; SEQUENCE DELETION; TRANSFORMATION, BACTERIAL; TRANSLOCATION, GENETIC; VIRULENCE;

AGROBACTERIUM; AGROBACTERIUM TUMEFACIENS; ANIMALIA; BACTERIA (MICROORGANISMS); EUKARYOTA; HELICOBACTER; HELICOBACTER PYLORI; INSERTION SEQUENCES; NEGIBACTERIA;

EID: 0035725211     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2958.2001.02714.x     Document Type: Article

References (40)

1. Genomic-sequence comparison of two unrelated isolates of the human gastric pathogen Helicobacter pylori
2. Helicobacter pylori CagA protein can be tyrosine phosphorylated in gastric epithelial cells
3. Implication of the structure of the Helicobacter pylori cag pathogenicity island in induction of interleukin-8 secretion
4. Translocation of the Helicobacter pylori CagA protein in gastric epithelial cells by a type IV secretion apparatus
5. A complementation analysis of the restriction and modification of DNA in Escherichia coli
6. Cag, a pathogenicity island of Helicobacter pylori, encodes type I-specific and disease-associated virulence factors
7. FACS-optimized mutants of the green fluorescent protein (gfp)
8. Tyrosine-phosphory-lated bacterial proteins: Trojan horses for the host cell
9. Helicobacter pylori virulence and genetic geography
10. Modulation of Helicobacter pylori induced interleukin-8 synthesis in gastric epithelial cells mediated by cag PAI encoded VirD4 homologue
11. Interleukin-1 polymorphisms associated with increased risk of gastric cancer
12. PAK1 activates the NIK-IKK NF-kappaB pathway and proinflammatory cytokines in H. pylori infection
13. Aflagellated mutants of Helicobacter pylori generated by genetic transformation of naturally competent strains using transposon shuttle mutagenesis
14. IgA protease of Neisseria gonorrhoeae: Isolation and characterization of the gene and its extracellular product
15. Clinical outcome after infection with Helicobacter pylori does not appear to be reliably predicted by the presence of any of the genes of the cag pathogenicity island
16. An improved TnMax mini-transposon system suitable for sequencing, shuttle mutagenesis and gene fusions
17. Helicobacter pylori infection activates NF-kappa B in gastric epithelial cells
18. Cleavage of structural proteins during the assembly of the head of the bacteriophage T4
19. Pathogenicity of Helicobacter pylori: A perspective
20. Characterisation of flaA, a Helicobacter pylori flagellin gene
21. Multiple genes in the left half of the cag pathogenicity island of Helicobacter pylori are required for tyrosine kinase-dependent transcription of interleukin-8 in gastric epithelial cells
22. Structure of cag pathogenicity island in Japanese Helicobacter pylori isolates
23. A secreted/shed product of Helicobacter pylori activates transcription factor nuclear factor-kappa B
24. Activation of activator protein 1 and stress response kinases in epithelial cells colonized by Helicobacter pylori encoding the cag pathogenicity island
25. Composition and gene expression of the cag pathogenicity island in Helicobacter pylori strains isolated from gastric carcinoma and gastritis patients in Costa Rica
26. Cloning and genetic characterization of Helicobacter pylori catalase and construction of a catalase-deficient mutant strain
27. Translocation of Helicobacter pylori CagA into gastric epithelial cells by type IV secretion
28. Interaction of Helicobacter pylori with professional phagocytes: Role of the cag pathogenicity island and translocation, phosphorylation and specific processing of CagA
29. Virulence factors of Helicobacter pylori responsible for gastric diseases in Mongolian gerbil
30. A plasmid system for high-level expression and in vitro processing of recombinant proteins
31. Role of adherence in interleukin-8 induction in Helicobacter pylori-associated gastritis
32. Comparison of CXC chemokines, ENA-78 and interleukin-8 expression in Helicobacter pylori-associated gastritis
33. Yersinia enterocolitica-induced interleukin-8 secretion by human intestinal epithelial cells depends on cell differentiation
34. Yersinia enterocolitica invasin protein triggers IL-8 production in epithelial cells via activation of Rel p65-p65 homodimers
35. Induction of host signal transduction pathways by Helicobacter pylori
36. Altered states: Involvement of phosphorylated CagA in the induction of host cellular growth changes by Helicobacter pylori
37. Tyrosine phosphorylation of the Helicobacter pylori CagA antigen after cag-driven host cell translocation
38. Cloning and genetic characterization of the Helicobacter pylori and Helicobacter mustelae flaB flagellin genes and construction of H. pylori flaA and flaB-negative mutants by electroporation-mediated allelic exchange
39. The complete genome sequence of the gastric pathogen Helicobacter pylori