Purification of eukaryotic MutL homologs from Saccharomyces cerevisiae using self-cleaving affinity technology

Protein Expression and Purification

Volumn 21, Issue 2, 2001, Pages 333-342

  Hall, Mark C ^a   Kunkel, Thomas A ^a  

^a NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ESCHERICHIA COLI; EUKARYOTA; SACCHAROMYCES; SACCHAROMYCES CEREVISIAE;

EID: 0035715213     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.2000.1379     Document Type: Article

References (32)

1. Mammalian DNA mismatch repair
2. Eukaryotic DNA mismatch repair
3. Isolation and characterization of the Escherichia coli mutL gene product
4. Crystal structure and ATPase activity of MutL: Implications for DNA repair and mutagenesis
5. The Escherichia coli MutL protein physically interacts with MutH and stimulates the MutH-associated endonuclease activity
6. Evidence for a physical interaction between the Escherichia coli methyl-directed mismatch repair proteins MutL and UvrD
7. MutS mediates hetero-duplex loop formation by a translocation mechanism
8. MutS and MutL activate helicase II in a mismatch-dependent manner
9. The MutL ATPase is required for mismatch repair
10. Meiotic pachytene arrest in MLH1-deficient mice
11. Male mice defective in the DNA mismatch repair gene PMS2 exhibit abnormal chromosome synapsis in meiosis
12. Involvement of mouse Mlh1 in DNA mismatch repair and meiotic crossing over
13. 2 cell cycle checkpoint
14. Dual requirement in yeast DNA mismatch repair for MLH1 and PMS1, two homologs of the bacterial mutL gene
15. The Saccharomyces cerevisiae MLH3 gene functions in MSH3-dependent suppression of frameshift mutations
16. Functional specificity of MutL homologs in yeast: Evidence for three Mlh1-based heterocomplexes with distinct roles during meiosis in recombination and mismatch correction
17. Restoration of mismatch repair to nuclear extracts of H6 colorectal tumor cells by a heterodimer of human MutL homologs
18. Enhancement of MSH2-MSH3-mediated mismatch recognition by the yeast MLH1-PMS1 complex
19. Analysis of yeast MSH2-MSH6 suggests that the initiation of mismatch repair can be separated into discrete steps
20. Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element
21. Tackling the protease problem in Saccharomyces cerevisiae
22. Mutator phenotypes conferred by MLH1 overexpression and by heterozygosity for mlh1 mutations
23. Functional analysis of human MutSα and MutSβ complexes in yeast
24. Cloning and nucleotide sequence of DNA mismatch repair gene PMS1 from Saccharomyces cerevisiae: Homology of PMS1 to procaryotic MutL and HexB
25. ATP-dependent assembly of a ternary complex consisting of a DNA mismatch and the yeast MSH2-MSH6 and MLH1-PMS1 protein complexes
26. Inactivation of DNA mismatch repair by increased expression of yeast MLH1
27. Protein splicing involving the Saccharomyces cerevisiae VMA intein: The steps in the pathway, side reactions leading to protein cleavage, and establishment of an in vitro splicing system
28. Identification of in-gel digested proteins by complementary peptide mass fingerprinting and tandem mass spectrometry data obtained on an electrospray ionization quadrupole time-of-flight mass spectrometer
29. + but not ATP hydrolysis
30. Storage of pure proteins
31. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
32. Functional domains of the Saccharomyces cerevisiae Mlh1p and Pms1p DNA mismatch repair proteins and their relevance to human hereditary nonpolyposis colorectal cancer-associated mutations