Design and characterization of a novel « family-shuffling » technology adapted to membrane enzyme: Application to P450s involved in xenobiotic metabolism

Advances in Experimental Medicine and Biology

Volumn 500, Issue , 2001, Pages 319-322

  Abécassis, Valérie ^a   Pompon, Denis ^a   Truan, Gilles ^a  

^a CNRS   (France)

Author keywords

[No Author keywords available]

Indexed keywords

CYTOCHROME P450; DNA; MEMBRANE ENZYME; XENOBIOTIC AGENT;

BIOTRANSFORMATION; CONFERENCE PAPER; CONTROLLED STUDY; DNA HYBRIDIZATION; DNA LIBRARY; EXPRESSION VECTOR; GENETIC ENGINEERING; NONHUMAN; OXIDATION REDUCTION POTENTIAL; PRIORITY JOURNAL; XENOBIOTIC METABOLISM; YEAST;

VECTORS;

EID: 0035697688     PISSN: 00652598     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4615-0667-6_49     Document Type: Conference Paper

References (11)

1. The role of cytochrome P-450 in the metabolism of chemical carcinogens
2. Engineering new functions and altering existing functions
3. Combinatorial protein design by in vitro recombination
4. Strategies for the in vitro evolution of protein function: Enzyme evolution by random recombination of improved sequences
5. Directed evolution of an aspartate aminotransferase with new substrate specificities
6. Directed evolution of new catalytic activity using the alpha/beta-barrel scaffold
7. DNA shuffling of a family of genes from diverse species accelerates directed evolution
8. An effective family shuffling method using single-stranded DNA
9. DNA shuffling by random fragmentation and reassembly: In vitro recombination for molecular evolution
10. Recombination between similar but not identical DNA sequences during yeast transformation occurs within short stretches of identity
11. Enhanced in vivo monooxygenase activities of mammalian P450s in engineered yeast cells producing high levels of NADPH-P450 reductase and human cytochrome b5