Interactions of mRNAs and gRNAs involved in trypanosome mitochondrial RNA editing: Structure probing of an mRNA bound to its cognate gRNA

RNA

Volumn 7, Issue 12, 2001, Pages 1803-1816

  Leung, S S ^a   Koslowsky, D J ^a  

^a Michigan State University ^*  (United States)

Author keywords

Apocytochrome b; Kinetoplasts; Organelle gene expression; Posttranscriptional processing; RNA structure; RNA RNA interactions

Indexed keywords

DOUBLE STRANDED RNA; EXONUCLEASE; GUIDE RNA; HEXOSE 1 PHOSPHATE URIDYLYLTRANSFERASE; MESSENGER RNA; MITOCHONDRIAL RNA;

3' UNTRANSLATED REGION; ARTICLE; BASE PAIRING; ENZYME ACTIVITY; MITOCHONDRIAL RESPIRATION; MOLECULAR RECOGNITION; NONHUMAN; PREDICTION; PRIORITY JOURNAL; PROTEIN CROSS LINKING; PROTEIN MOTIF; PROTEIN RNA BINDING; PROTEIN SECONDARY STRUCTURE; RNA BINDING; RNA EDITING; RNA SEQUENCE; RNA STRUCTURE; TRANSCRIPTION REGULATION; TRYPANOSOMA;

ANIMALS; APOPROTEINS; ASPERGILLUS NUCLEASE S1; BASE SEQUENCE; CYTOCHROME B GROUP; CYTOCHROMES B; ENDORIBONUCLEASES; FORECASTING; MOLECULAR SEQUENCE DATA; NUCLEIC ACID CONFORMATION; RIBONUCLEASE T1; RNA; RNA EDITING; RNA, DOUBLE-STRANDED; RNA, GUIDE; RNA, MESSENGER; SUBSTRATE SPECIFICITY; TRYPANOSOMA;

TRYPANOSOMA;

EID: 0035671634     PISSN: 13558382     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (40)

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2. Guide RNAs in kinetoplastid mitochondria have a nonencoded 3′ oligo(U) tail involved in recognition of the preedited region
3. Kinetoplastid RNA editing does not require the terminal 3′ hydroxyl of guide RNA, but modifications to the guide RNA terminus can inhibit in vitro U insertion
4. Mapping the active site of ribonuclease P RNA using a substrate containing a photoaffinity agent
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6. Analysis of rRNA structure: Experimental and theoretical considerations
7. Complexes from Trypanosoma brucei that exhibit deletion editing and other editing-associated properties
8. Unexpected electrophoretic migration of RNA with different 3′ termini causes a RNA sizing ambiguity that can be resolved using nuclease P1-generated sequencing ladders
9. Trypanosome U-deletional RNA editing involves guide RNA-directed endonuclease cleavage, terminal U exonuclease, and RNA ligase activities
10. Probing the structure of RNAs in solution
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13. Developmentally regulated addition of nucleotides within apocytochrome b transcripts in Trypanosoma brucei
14. The formation of mitochondrial ribonucleoprotein complexes involving guide RNA molecules in Trypanosoma brucei
15. Mitochondrial mRNA editing in kinetoplastid protozoa
16. Cleavage of DNA with methidiumpropyl-EDTA-iron(II): Reaction conditions and product analyses
17. Uridylate addition and RNA ligation contribute to the specificity of kinetoplastid insertion RNA editing
18. RNA editing: A mechanism for gRNA-specified uridylate insertion into precursor mRNA
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20. Detection of high-affinity intercalator sites in a ribosomal RNA fragment by the affinity cleavage intercalator methidiumpropyl-EDTA-iron(II)
21. Distinct differences in the requirements for ribonucleoprotein complex formation on differentially regulated pre-edited mRNAs in Trypanosoma brucei
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26. RNAdraw: An integrated program for RNA secondary structure calculation and analysis under 32-bit Microsoft Windows
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28. Association of two novel proteins, TbMP52 and TbMP48, with the Trypanosoma brucei RNA editing complex
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30. Native mRNA editing complexes from Trypanosoma brucei mitochondria
31. Assembly of mitochondrial ribonucleoprotein complexes involves specific guide RNA (gRNA)-binding proteins and gRNA domains but does not require preedited mRNA
32. Multiple guide RNAs for identical editing of Trypanosoma brucei apocytochrome b mRNA have an unusual minicircle location and are developmentally regulated
33. Purification of a functional enzymatic editing complex from Trypanosoma brucei mitochondria
34. The secondary structure of guide RNA molecules from Trypanosoma brucei
35. Sequence-specific double-strand cleavage of DNA by penta-N-methylpyrrolecarboxamide-EDTA X Fe(II)
36. Direct visualization of uridylate deletion in vitro suggests a mechanism for kinetoplastid RNA editing
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38. The specificity of ribonuclease T2
39. RNA structure analysis using methidiumpropyl-EDTA.Fe(II): A base-pair-specific RNA structure probe
40. Photochemical labeling of bovine pancreatic ribonuclease A with 8-azidoadenosine 3′,5′-bisphosphate