Efficient Homolactic Fermentation by Kluyveromyces lactis Strains Defective in Pyruvate Utilization and Transformed with the Heterologous LDH Gene

Applied and Environmental Microbiology

Volumn 67, Issue 12, 2001, Pages 5621-5625

  Bianchi, Michele M ^a   Brambilla, Luca ^b   Protani, Francesca ^a   Liu, Chi Li ^c   Lievense, Jefferson ^c   Porro, Danilo ^b  

^a SAPIENZA UNIVERSITY OF ROME   (Italy)

^b UNIVERSITY OF MILANO BICOCCA   (Italy)

^c Tate and Lyle Speciality Sweeteners   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

LACTATE DEHYDROGENASE; LACTIC ACID; PYRUVATE DECARBOXYLASE; PYRUVIC ACID;

ARTICLE; CULTURE MEDIUM; ENZYMOLOGY; FERMENTATION; GENE DELETION; GENETIC TRANSFORMATION; GENETICS; GROWTH, DEVELOPMENT AND AGING; KLUYVEROMYCES; METABOLISM;

CULTURE MEDIA; FERMENTATION; GENE DELETION; KLUYVEROMYCES; LACTATE DEHYDROGENASE; LACTIC ACID; PYRUVATE DECARBOXYLASE; PYRUVIC ACID; SUPPORT, NON-U.S. GOV'T; TRANSFORMATION, GENETIC;

EID: 0035656862     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.67.12.5621-5625.2001     Document Type: Article

References (20)

1. Adachi, E., M. Torigoe, M. Sugiyama, J. J. Nikawa, and K. Shimizu. 1998. Modification of metabolic pathways of Saccharomyces cerevisiae by the expression of lactate dehydrogenase and deletion of pyruvate decarboxylase genes for the lactic acid fermentation at low pH value. J. Ferment. Bioeng. 86:284-289.
2. Benninga, H. A. 1990. A history of lactic acid making. Kluyver Academic Publishers, Dordrecht, The Netherlands.
3. Bianchi, M. M., L. Tizzani, M. Destruelle, L. Frontali, and M. Wésolowski-Louvel. 1996. The "petite-negative" yeast Kluyveromyces lactis has a single gene expressing pyruvate decarboxylase activity. Mol. Microbiol. 19:27-36.
4. Buchta, K. 1983. Lactic acid, p. 409-417. In H. Delleweg (ed.), Bio/technology, vol. 3. Verlag Chemie, Weinheim, Germany.
5. Dequin, S., and P. Barre. 1994. Mixed lactic acid-alcoholic fermentation by Saccharomyces cerevisiae expressing the Lactobacillus casei L-(+)-LDH. Bio/ Technology 12:173-177.
6. Destruelle, M., R. Menghini, L. Frontali, and M. M. Bianchi. 1999. Regulation of the expression of the Kluyveromyces lactis PDC1 gene: carbon source responsive elements and autoregulation. Yeast 15:361-370.
7. Gatignol, A., M. Dassain, and G. Tirabi. 1990. Cloning of Saccharomyces cerevisiae promoters using a probe vector based on the phleomycin resistance. Gene 9:35-41.
8. Gonzales-Siso, M. I., E. Ramil, M. E. Cerdan, and M. A. Freire-Picos. 1996. Respirofermentative metabolism in Kluyveromyces lactis: ethanol production and the Crabtree effect. Enzyme Microbiol. Technol. 18:585-591.
9. Hohmann, S. 1991. Characterization of PDC6, a third structural gene for pyruvate decarboxylase in Saccharomyces cerevisiae. J. Bacteriol. 173:7963-7969.
10. Kongo, M., Y. Nomura, and M. Iwahara. 1986. Novel methods of lactic acid production by electrodialysis fermentation. Appl. Environ. Microbiol. 32: 227-234.
11. Kiers, J., A.-M. Zeeman, M. Luttik, C. Thiele, J. I. Castrillo, Y. H. Steensma, J. P. Van Dijken, and J. T. Pronk. 1997. Regulation of alcoholic fermentation in batch and chemostat cultures of Kluyveromyces lactis CBS2359. Yeast 14:459-469.
12. Morlino, G. B., L. Tizzani, R. Fleer, L. Frontali, and M. M. Bianchi. 1999. Inducible amplification of gene copy number and heterologous protein production in the yeast Kluyveromyces lactis. Appl. Environ. Microbiol. 65:4808-4813.
13. Mulder, W., I. H. J. M. Scholten, and L. A. Grivell. 1995. Carbon catabolite regulation of transcription of nuclear genes coding for mitochondrial proteins in Kluyveromyces lactis. Curr. Genet. 28:267-273.
14. Porro, D., L. Brambilla, B. M. Ranzi, E. Martegani, and L. Alberghina. 1995. Development of metabolically engineered Saccharomyces cerevisiae cells for the production of lactic acid. Biotechnol. Prog. 11:294-298.
15. Porro, D., M. M. Bianchi, L. Brambilla, R. Menghini, D. Bolzani, V. Carrera, J. Lievense, C.-L. Liu, B. M. Ranzi, L. Frontali, and L. Alberghina. 1999. Replacement of a metabolic pathway for large-scale production of lactic acid from engineered yeasts. Appl. Environ. Microbiol. 65:4211-4215.
16. Schaaff. I., J. B. A. Green, D. Gozalbo, and S. Hohmann. 1989. A deletion of the PDC1 gene coding for pyruvate decarboxylase of yeast causes a different phenotype than previously isolated point mutations. Curr. Genet. 15:75-81.
17. Schmitt, H. D., M. Ciriacy, and F. K. Zimmermann. 1983. The synthesis of yeast pyruvate decarboxylase is regulated by large variations in the messenger RNA level. Mol. Gen. Genet. 192:247-252.
18. Wésolowski-Louvel, M., C. Prior, D. Bornecque, and H. Fukuhara. 1992. Rag mutations involved in glucose metabolism in yeast: isolation and genetic characterization. Yeast 8:711-719.
19. Wésolowski-Louvel, M., and H. Fukuhara. 1995. A map of the Kluyveromyces lactis genome. Yeast 11:211-218.
20. Zeeman, A.-M., M. A. H. Luttik, C. Thiele, J. P. Van Dijken, J. T. Pronk, and Y. H. Steensma. 1998. Inactivation of the Kluyveromyces lactis KIPDAI gene leads to loss of pyruvate dehydrogenase activity, impairs growth on glucose and triggers alcoholic fermentation. Microbiology 144:3437-3446.