Involvement of the c-terminal end of the prostrate-specific antigen in a conformational epitope: Characterization by proteolytic degradation of monoclonal antibody-bound antigen and mass spectrometry

Journal of Molecular Recognition

Volumn 14, Issue 6, 2001, Pages 406-413

  Michel, Sandrine ^a   Forest, Eric ^b   Pétillot, Yves ^b   Deléage, Gilbert ^c   Heuzé Vourc'h, Nathalie ^d   Courty, Yves ^d   Lascoux, David ^b   Jolivet, Michel ^a   Jolivet Reynaud, Colette ^a  

^a BIOMÉRIEUX   (France)

^b INSTITUT DE BIOLOGIE STRUCTURALE   (France)

^c INSTITUT DE BIOLOGIE ET CHIMIE DES PROTÉINES   (France)

^d INSERM   (France)

Author keywords

Epitope mapping; Mass spectrometry; Monoclonal antibodies; Prostate specific antigen; Protein modeling

Indexed keywords

CHYMOTRYPSIN; CHYMOTRYPSIN A; EPITOPE; MONOCLONAL ANTIBODY; PROSTATE SPECIFIC ANTIGEN; RECOMBINANT PROTEIN; TRYPSIN;

AMINO ACID SEQUENCE; ANTIBODY AFFINITY; ARTICLE; CHEMISTRY; EPITOPE MAPPING; GENETICS; IMMUNOLOGY; MASS SPECTROMETRY; METABOLISM; METHODOLOGY; MOLECULAR GENETICS; PROTEIN CONFORMATION;

AMINO ACID SEQUENCE; ANTIBODIES, MONOCLONAL; ANTIBODY AFFINITY; CHYMOTRYPSIN; EPITOPE MAPPING; EPITOPES; MOLECULAR SEQUENCE DATA; PROSTATE-SPECIFIC ANTIGEN; PROTEIN CONFORMATION; RECOMBINANT PROTEINS; SPECTRUM ANALYSIS, MASS; TRYPSIN;

EID: 0035542873     PISSN: 09523499     EISSN: None     Source Type: Journal    
DOI: 10.1002/jmr.552     Document Type: Article

References (34)

1. Three-dimensional structure of an antigen-antibody complex at 2.8 Å resolution
2. Two-dimensional electrophoresis of prostate-specific antigen in sera of men with prostate cancer or benign prostate hyperplasia
3. Enzymatic activity of prostate-specific antigen and its reactions with extracellular serine proteinase inhibitors
4. Structure and chromosomal localization of the human renal kallikrein gene
5. Expression of the gene coding for human prostate-specific antigen and related hGK-1 in benign and malignant tumors of the human prostate
6. Molecular cloning and expression of an alternative hKLK3 transcript coding for a variant protein of prostate-specific antigen
7. Molecular heterogeneity of free PSA in sera of patients with benign and malignant prostate tumours
8. Characterization of a discontinuous epitope of the human immunodeficiency virus (HIV) core protein p24 by epitope excision and differential chemical modification followed by mass spectrometric peptide mapping analysis
9. Mass spectrometric characterization of a discontinuous epitope of the HIV envelope protein HIV-gp 120 recognized by the human monoclonal antibody 1331A
10. Mapping epitopes on a protein antigen by the proteolysis of antigen-antibody complexes
11. Matrix-assisted laser desorption ionization/mass spectrometry mapping of human immunodeficiency virus-gp120 epitopes recognized by a limited polyclonal antibody
12. Direct identification of protein epitopes by mass spectrometry without immobilization of antibody and isolation of antibody-peptide complexes
13. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
14. Characterization of an anti-Borrelia burgdorferi OspA conformational epitope by limited proteolysis of monoclonal antibody-bound antigen and mass spectrometric peptide mapping
15. A kallikrein-like serine protease in prostatic fluid cleaves the predominant seminal vesicle protein
16. Molecular cloning of human prostate-specific antigen cDNA
17. Mass spectrometric mapping of protein epitope structures of myocardial infarct markers myoglobin and troponin T
18. Anti-free prostate-specific antigen monoclonal antibody epitopes defined by mimotopes and molecular modeling
19. Structural basis for the binding of an anti-cytochrome c antibody to its antigen: Crystal structures of FabE8-cytochrome c complex to 1.8 Å resolution and FabE8 to 2.26 Å resolution
20. Isolation and characterization of free form prostate-specific antigen (f-PSA) in sera of men with prostate cancer
21. Epitope mapping by mass spectrometry: Determination of an epitope on HIV-1 IIIB p26 recognized by a monoclonal antibody
22. Molecular cloning and characterization of novel prostate antigen cDNA's
23. Prostate-specific antigen as a serum marker for adenocarcinoma of the prostate
24. Molecular epitope identification by limited proteolysis of an immobilized antigen-antibody complex and mass spectrometric peptide mapping
25. A novel form of prostate-specific antigen transcript produced by alternative splicing
26. A structural model for the prostate disease marker, human prostate-specific antigen
27. Structural investigation of the alpha-1-antichymotrypsin: Prostate-specific antigen complex by comparative model building
28. Human prostate-specific antigen: Structural and functional similarity with serine proteases
29. Mapping epitopes of monoclonal antibodies against HIV-1 integrase with limited proteolysis and matrix-assisted laser desorption ionization time-of-flight mass spectrometry
30. An inhibitory monoclonal antibody binds at the turn of the helix-turn-helix motif in the N-terminal domain of HIV-1 integrase
31. Epitope mapping of monoclonal antibodies by mass spectrometry: Identification of protein antigens in complex biological systems
32. Purification and characterization of different molecular forms of prostate-specific antigen in human seminal fluid
33. Protein epitope mapping by mass spectrometry
34. Mapping protein-protein interactions by affinity-directed mass spectrometry