Molecular cloning and expression of an alternative hKLK3 transcript coding for a variant protein of prostate-specific antigen

Cancer Research

Volumn 59, Issue 12, 1999, Pages 2820-2824

  Heuzé, Nathalie ^a   Olayat, Sophie ^a   Gutman, Ninette ^a   Zani, Marie Louise ^a   Courty, Yves ^a,b  

^a UNIVERSITÉ DE TOURS   (France)

^b Lab d'Enzymol Chim des Proteines   (France)

Author keywords

[No Author keywords available]

Indexed keywords

PROSTATE SPECIFIC ANTIGEN;

ARTICLE; CELL GROWTH; DIAGNOSTIC VALUE; HUMAN; HUMAN CELL; MOLECULAR CLONING; PRIORITY JOURNAL; PROSTATE CANCER; PROTEIN EXPRESSION; TISSUE SPECIFICITY;

ALTERNATIVE SPLICING; AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; CLONING, MOLECULAR; COS CELLS; CROSS REACTIONS; HUMANS; MOLECULAR SEQUENCE DATA; PROSTATE-SPECIFIC ANTIGEN; RECOMBINANT FUSION PROTEINS; RNA, MESSENGER; TUMOR CELLS, CULTURED;

EID: 0033564485     PISSN: 00085472     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (21)

1. McCormack, R. T., Rittenhouse, H. G., Finlay, J. A., Sokolof, R. L., Wang, T. J., Wolfert, R. L., Lilja, H., and Oesterling, J. E. Molecular forms of prostate-specific antigen and the human kallikrein gene family: a new era. Urology, 45: 729-744, 1995.
2. Christensson, A., Laurell, C. B., and Lilja, H. Enzymatic activity of prostate-specific antigen and its reactions with extracellular serine proteinase inhibitors. Eur. J. Biochem., 194: 755-763, 1990.
3. Lilja, H. A kallikrein-like serine protease in prostatic fluid cleaves the predominant seminal vesicle protein. J. Clin. Invest., 76: 1899-1903, 1985.
4. Cohen, P., Graves, H., Peehl, D., Kamarei, M., Giudice, L., and Rosenfeld, R. Prostate-specific antigen (PSA) is an insulin-like growth factor binding protein-3 protease found in seminal plasma. J. Clin. Endocrinol. Metab., 75: 1046-1053, 1992.
5. Stamey, T. A., Ekman, P. E., Blankenstein, M. A., Cooper, E. H., Kontturi, M., Lilja, H., Oesterling, J. E., Stenman, U. H., and Turkes, A. Tumor markers. Consensus Conference on Diagnosis and Prognostic Parameters in Localized Prostate Cancer. Stockholm, Sweden, May 12-13, 1993. Scand. J. Urol. Nephrol. Suppl., 162: 73-87, 1994.
6. Henttu, P., Lukkarinen, O., and Vihko, P. Expression of the gene coding for human prostate-specific antigen and related hGK-1 in benign and malignant tumors of the human prostate. Int. J. Cancer, 45: 654-660, 1990.
7. Singer, M., Courty, Y., and Rougeon, F. Recent evolution of genes encoding the prohormone-like protein SMRI in the rat submandibular gland. DNA Cell Biol., 14: 137-144, 1995.
8. Takayama, T. K., Fujikawa, K., and Davie, E. W. Characterization of the precursor of prostate-specific antigen. Activation by trypsin and by human glandular kallikrein. J. Biol. Chem., 272: 21582-21588, 1997.
9. Riegman, P. H. J., Klaassen, P., Van der Korput, J. A. G. M., Romijn, J. C., and Trapman, J. Molecular cloning and characterization of novel prostate antigen cDNAs. Biochem. Biophys. Res. Commun., 155: 181-188, 1988.
10. Keil, B. Specificity of Proteolysis, pp. 54-56. Berlin: Springer-Verlag, 1992.
11. Sheets, M. D., Ogg, S. C., and Wickens, M. P. Point mutations in AAUAAA and the poly (A) addition site: effects on the accuracy and efficiency of cleavage and polyadenylation in vitro. Nucleic Acids Res., 18: 5799-5805, 1990.
12. Henttu, P., Liao, S., and Vihko, P. Androgens up-regulate the human prostate-specific antigen messenger ribonucleic acid (mRNA), but down-regulate the prostatic acid phosphatase mRNA in the LNCaP cell line. Endocrinology, 130: 766-772, 1992.
13. Wolf, D., Schulz, P., and Fittler, F. Transcriptional regulation of prostate kallikrein-like genes by androgen. Mol. Endocrinol., 6: 753-762, 1992.
14. Kumar, A., Mikolajczyk, S. D., Goel, A. S., Millar, L. S., and Saedi, M. S. Expression of pro form of prostate-specific antigen by mammalian cells and its conversion to mature, active form by human kallikrein 2. Cancer Res., 57; 3111-3114, 1997.
15. Qiu, S. D., Young, C. Y., Bilhartz, D. L., Prescott, J. L., Farrow, G. M., He, W. W., and Tindall, D. J. In situ hybridization of prostate-specific antigen mRNA in human prostate. J. Urol., 144: 1550-1556, 1990.
16. Christensson, A., Bjartell, A., and Lilja, H. The significance of serpins in the regulation of proteases in the male genital tract. In: Church, F. C., Cunningham, D. D., Ginsburg, D., Hoffman, M., Stone, S. R., and Tollefsen, D. M. (eds.), Chemistry and Biology of Serpins, pp. 163-176. New York: Plenum Press, 1997.
17. Lilja, H., Christensson, A., Dahlen, U., Matikainen, M-T., Nilsson, O., Pettersson, K., and Lövgren, T. Prostate-specific antigen in serum occurs predominantly in complex with α1-antichymotrypsin. Clin. Chem., 37: 1618-1625, 1991.
18. Lilja, H. Regulation of the enzymatic activity of prostate-specific antigen and its reactions with extracellular protease inhibitors in prostate cancer. Scand. J. Clin. Lab. Invest. Suppl., 220: 47-56, 1995.
19. Noldus, J., Chen, Z., and Stamey, T. A. Isolation and characterization of free form prostate specific antigen (f-PSA) in sera of men with prostate cancer. J. Urol., 158: 1606-1609, 1997.
20. Mikolajczyk, S. D., Grauer, L. S., Millar, L. S., Hill, T. M., Kumar, A., Rittenhouse, H. G., Wolfert, R. L., and Saedi, M. S. A precursor form of PSA (pPSA) is a component of the free PSA in prostate cancer serum. Urology, 50: 710-714, 1997.
21. Becker, C., and Lilja, H. Individual prostate-specific antigen (PSA) forms as prostate tumor markers. Clin. Chim. Acta, 257: 117-132, 1997.