Isolation and characterization of free form prostate specific antigen (f-PSA) in sera of men with prostate cancer

Journal of Urology

Volumn 158, Issue 4, 1997, Pages 1606-1609

  Noldus, Joachim ^a   Chen, Zuxiong ^a   Stamey, Thomas A ^a  

^a STANFORD UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Free; Prostate cancer; Prostate specific antigen; PSA; Uncomplexed PSA

Indexed keywords

ENZYME PRECURSOR; PROSTATE SPECIFIC ANTIGEN;

AMINO TERMINAL SEQUENCE; ARTICLE; BONE METASTASIS; CONTROLLED STUDY; GEL FILTRATION CHROMATOGRAPHY; HUMAN; MAJOR CLINICAL STUDY; MALE; PRIORITY JOURNAL; PROSTATE CARCINOMA; PROTEIN ISOLATION; SEMINAL PLASMA;

EID: 0030822246     PISSN: 00225347     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-5347(01)64289-7     Document Type: Article

References (25)

1. Belanger, A., van Halbeek, H., Graves, H. C. B., Grandbois, K., Stamey, T. A., Huang, L., Poppe, I. and Labrie, F.: Molecular mass and carbohydrate structure of prostate specific antigen: Studies for establishment of an international PSA standard. Prostate, 27: 187, 1995.
2. Iwakiri, J., Grandbois, K., Wehner, N., Graves, H. C. B. and Stamey, T. A.: An analysis of urinary prostate specific antigen before and after radical prostatectomy: evidence for secretion of prostate specific antigen by the periurethral glands. J. Urol., 149: 783, 1993.
3. Wang, M. C., Valenzuela, L. A., Murphy, G. P. and Chu, T. M.: Purification of a human prostate specific antigen. Invest. Urol., 17: 159, 1979.
4. Lilja, H., Oldbring, J., Rannevik, G. and Laurell, C. B.: Seminal vesical-secreted proteins and their reactions during gelation and liquefaction of human semen. J. Clin. Invest., 80: 281, 1987.
5. Oesterling, J. E., Chan, D. W., Epstein, J. I., Kimball Jr., A. W., Bruzek, D. J., Rock, R. C., Brendler, C. B. and Walsh, P. C.: Prostate specific antigen in the preoperative and postoperative evaluation of localized prostatic cancer treated with radical prostatectomy. J. Urol., 139: 766, 1988.
6. Stamey, T. A., Kabalin J. N., McNeal, J. E., Johnstone, I. M., Freiha, F., Redwine, E. A. and Yang, N.: Prostate specific antigen in the diagnosis of adenocarcinoma of the prostate. II. Radical prostatectomy treated patients. J. Urol., 141: 1076, 1989.
7. Stamey, T. A., Kabalin, J. N., Ferrari, M. and Yang, M.: Prostate specific antigen in the diagnosis and treatment of adenocarcinoma of the prostate. IV. Anti-androgen treated patients. J. Urol., 141: 1088, 1989.
8. Stamey, T. A., Ferrari, M. K. and Schmid, H.-P.: The value of serial prostate specific antigen determinations 5 years after radiotherapy: Steeply increasing values characterize 80% of patients. J. Urol., 150: 1856, 1993.
9. Partin, A. W., Pound, C. R., Clemens, J. Q., Epstein, J. I. and Walsh, P. C.: Serum PSA after anatomic radical prostatectomy. The Johns Hopkins experience after 10 years. Urol. Clin. North Am., 20: 713, 1993.
10. Stenman, U. H., Leinonen, J., Alfthan, H., Rannikko, S., Tuhkanen, K. and Alfthan, O.: A complex between prostate-specific antigen and alpha 1-antichymotrypsin is the major form of prostate-specific antigen in serum of patients with prostatic cancer: assay of the complex improves clinical sensitivity for cancer. Cancer Res. 51: 222, 1991.
11. Lilja, H., Christensson, A., Dahlen, U., Matikainen, M.-T., Nilsson, O., Pettersson, K. and Lšvgren, T.: Prostate-specific antigen in serum occurs predominantly in complex with a-1-antichymotrypsin. Clin. Chem., 37: 1618, 1991.
12. Stamey, T. A., Chen, Z. and Prestigiacomo, A.: Serum prostate specific antigen binding a-1-antichymotrypsin: influence of cancer volume, location and therapeutic selection of resistant clones. J. Urol., 152: 1510, 1994.
13. Sensabaugh, G. F.: Isolation and characterization of a semen-specific protein from human seminal plasma: a potential new marker for semen identification. J. Forensic Sci., 23: 106, 1978.
14. Christensson, A., Laurell, C. B. and Lilja, H.: Enzymatic activity of prostate-specific antigen and its reaction with extracellular serine proteinase inhibitors. Eur. J. Biochem., 194: 755, 1990.
15. Riegman, P. H. J., Vlietstra, R. J., Fan der Korput, J. A. G. M., Romojin, J. C. and Trapman, J.: Characterization of the prostate-specific antigen gene. Biochem. Biophys. Res. Comm., 159: 95, 1989.
16. Laemmli, U. K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227: 680, 1970.
17. Schagger, H. and von Jagow, G.: Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for separation of proteins in the range from 1 to 100 kDa. Anal. Biochem., 166: 368, 1987.
18. Hames, B. D.: One-dimensional polyacrylamide gel electrophoresis. In: Gel Electrophoresis of Proteins, A Practical Approach. Edited by B. D. Hames and D. Rickwood, pp. 1-139, IRL Press, N.Y., 1990.
19. Schaller, J., Akiyama, K., Tsuda, R., Hara, M., Marti, T. and Rickli, E. E. Isolation, characterization and ammo-acid sequence of gamma-seminoprotein, a glycoprotein from human seminal plasma. Eur. J. Biochem., 170: 111, 1987.
20. Sensabough, G. E. and Blake, E. T.: Seminal plasma protein: Simplified purification and evidence for identity with prostate specific antigen. J. Urol., 144: 1523, 1990.
21. Christensson, A., Bjšrk, T., Nilsson, O., Dahlen, U., Martikainen, M.-T., Cockett, A. T. K., Abrahamsson, P.-A. and Lilja, H.: Serum prostate specific antigen complexed to a-1-antichymotrypsin as an indicator of prostate cancer. J. Urol., 150: 100, 1993.
22. Lilja, H.: A kallikrein-like serine protease in prostatic fluid cleaves the predominant seminal vesicle protein. J. Clin. Invest., 76: 1899, 1985.
23. Hammerer, P. G., McNeal, J. E. and Stamey, T. A.: Correlation between serum PSA levels and the volume of the individual glandular zones of the human prostate. J. Urol., 153: 111, 1995.
24. Chen, Z., Chen, H. and Stamey, T. A.: Prostate specific antigen (PSA) in benign prostatic hyperplasia (BPH) tissue is different from PSA in prostate cancer (PCa) tissue. J. Urol., 155: 1541, 1996.
25. 1. antichymotrypsin production in PSA producing cells is common in prostate cancer but rare in benign prostatic hyperplasia. Urology, 43: 427, 1994.