P-glycoprotein does not reduce substrate concentration from the extracellular leaflet of the plasma membrane in living cells

Cancer Research

Volumn 61, Issue 21, 2001, Pages 7763-7769

  Chen, Yu ^a   Pant, Alok C ^a   Simon, Sanford M ^a  

^a ROCKEFELLER UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CYCLOSPORIN A; GLYCOPROTEIN P; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; TRIMETHYLAMINE;

ARTICLE; CELL MEMBRANE; CONCENTRATION RESPONSE; CONTROLLED STUDY; ENZYME SPECIFICITY; EXTRACELLULAR SPACE; FLUORESCENCE MICROSCOPY; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; INCUBATION TIME; PRIORITY JOURNAL; PROTEIN EXPRESSION;

CELL MEMBRANE; CYTOPLASM; DIPHENYLHEXATRIENE; DRUG RESISTANCE, MULTIPLE; DRUG RESISTANCE, NEOPLASM; EXTRACELLULAR SPACE; FLUORESCENT DYES; GREEN FLUORESCENT PROTEINS; HELA CELLS; HUMANS; LUMINESCENT PROTEINS; P-GLYCOPROTEIN; RECOMBINANT FUSION PROTEINS; SUBSTRATE SPECIFICITY; TRANSFECTION;

EID: 0035503608     PISSN: 00085472     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (45)

1. High-dose chemotherapy. Concepts and strategies
2. Cell biological mechanisms of multidrug resistance in tumors
3. Clinical multidrug resistance in cancer: A multifactorial problem
4. Biochemistry of multidrug resistance mediated by the multidrug transporter
5. P-glycopretein-mediated multidrug resistance in tumor cells: Biochemistry, clinical relevance and modulation
6. Multidrug resistance: Molecular mechanisms and clinical relevance
7. Overexpression of a transporter gene in a multidrug-resistant human lung cancer cell line
8. A multidrug resistance transporter from human MCF-7 breast cancer cells
9. Two members of the mouse mdr gene family confer mulfidrug resistance with overlapping but distinct drug specificities
10. In situ biochemical demonstration that P-glycoprotein is a drug efflux pump with broad specificity
11. Kinetics of the multidrug transporter (P-glycoprotein) and its reversal
12. Mechanism of action of P-glycoprotein in relation to passive membrane permeation
13. MDRI P-glycoprotein is a lipid translocase of broad specificity, while MDR3 P-glycoprotein specifically translocates phosphatidylcholine
14. Physical-chemical properties shared by compounds that modulate multidrug resistance in human leukemic cells
15. Transport studies of doxorubicin in model membranes indicate a difference in passive diffusion across and binding at the outer and inner leaflets of the plasma membrane
16. The role of passive transbilayer drug movement in multidrug resistance and its modulation
17. Flip-flop of doxorubicin across erythrocyte and lipid membranes
18. Transmembrane aromatic amino acid distribution in P-glycoprotein. A functional role in broad substrate specificity
19. Evidence for two nonidentical drug-interaction sites in the human P-glycoprotein
20. An altered pattern of cross-resistance in multidrug-resistant human cells results from spontaneous mutations in the mdrl (P-glycoprotein) gene
21. Biochemical, cellular, and pharmacological aspects of the multidrug transporter
22. The transmembrane domains of the human multidrug resistance P-glycoprotein are sufficient to mediate drug binding and trafficking to the cell surface
23. P-glycoprotein-mediated Hoechst 33342 transport out of the lipid bilayer
24. Photosensitized labeling of a functional multidrug transporter in living drug-resistant tumor cells
25. Is the multidrug transporter a flippase?
26. Extraction of Hoechst 33342 from the cytoplasmic leaflet of the plasma membrane by P-glycoprotein
27. Defective acidification in human breast tumor cells and implications for chemotherapy
28. Tamoxifen inhibits acidification in cells independent of the estrogen receptor
29. Segregation of transferrin to a mildly acidic (pH 6.5) para-Golgi compartment in the recycling pathway
30. Cell surface membrane homeostasis and intracellular membrane traffic balance in mouse L929 cells
31. The kinetic aspects of intracellular fluorescence labeling with TMA-DPH support the maturation model for endocytosis in L929 cells
32. TMA-DPH: A suitable fluorescence polarization probe for specific plasma membrane fluidity studies in intact living cells
33. Multidrug resistance in Lactococcus lactis: Evidence for ATP-dependent drug extrusion from the inner leaflet of the cytoplasmic membrane
34. Localization of mitochondria in living cells with rhodamine 123
35. Isolation of amplified and overexpressed DNA sequences from adriamycin-resistant human breast cancer cells
36. Defective pH regulation of acidic compartments in human breast cancer cells (MCF-7) is normalized in adriamycin-resistant cells (MCF-7adr)
37. Active outward transport of daunomycin in resistant Ehrlich ascites tumor cells
38. Reduced permeability in CHO cells as a mechanism of resistance to colchicine
39. Fluorescent cellular indicators are extruded by the multidrug resistance protein
40. Kinetic analysis of calcein and calcein-acetoxymethylester efflux mediated by the multidrug resistance protein and P-glycoprotein
41. Relation between the turnover number for vinblastine transport and for vinblastine-stimulated ATP hydrolysis by human P-glycoprorein
42. Functional reconstitution of P-glycoprotein reveals an apparent near stoichiometric drug transport to ATP hydrolysis
43. Reconstitution of drug-stimulated ATPase activity following co-expression of each half of human P-glycoprotein as separate polypeptides
44. ATP hydrolysis by multidrug-resistance protein from Chinese hamster ovary cells
45. ATPase activity of purified and reconstituted P-glycoprotein from Chinese hamster ovary cells