Clue to damage recognition by UvrB: Residues in the β-hairpin structure prevent binding to non-damaged DNA

EMBO Journal

Volumn 20, Issue 21, 2001, Pages 6140-6149

  Moolenaar, Geri F ^a   Höglund, Lotta ^a   Goosen, Nora ^a  

^a LEIDEN UNIVERSITY   (Netherlands)

Author keywords

Damage recognition; DNA repair; Nucleotide flipping; UvrB

Indexed keywords

NUCLEOTIDE; SINGLE STRANDED DNA BINDING PROTEIN;

AMINO ACID SUBSTITUTION; ARTICLE; CRYSTAL STRUCTURE; DNA BINDING; DNA DAMAGE; DNA HELIX; DNA PROTEIN COMPLEX; DNA REPAIR; DNA STRAND; DNA STRUCTURE; HYDROPHOBICITY; MOLECULAR MODEL; PRIORITY JOURNAL; PROTEIN PURIFICATION;

AMINO ACID SUBSTITUTION; BACILLUS; BINDING SITES; DNA DAMAGE; DNA HELICASES; DNA REPAIR; DNA, BACTERIAL; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; MACROMOLECULAR SUBSTANCES; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; NUCLEIC ACID CONFORMATION; PLASMIDS; PROTEIN STRUCTURE, SECONDARY; SEQUENCE HOMOLOGY, AMINO ACID; STRUCTURE-ACTIVITY RELATIONSHIP; ULTRAVIOLET RAYS;

BACTERIA (MICROORGANISMS);

EID: 0035503314     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/20.21.6140     Document Type: Article

References (33)

1. Damage recognition in nucleotide excision repair of DNA
2. Structural basis for recognition and repair of the endogenous mutagen 8-oxoguanine in DNA
3. Mutagenic specificity in the lacI gene of Escherichia coli
4. The limited strand-separating activity of the UvrAB protein complex and its role in the recognition of DNA damage
5. Structure of the DNA repair enzyme endonuclease IV and its complex: Double-nucleotide flipping at abasic sites and three-metalion catalysis
6. Hepatitis C virus NS3 RNA helicase domain with a bound oligonucleotide: The crystal structure provides insights into the mode of unwinding
7. Major domain swiveling revealed by the crystal structures of complexes of E.coli Rep helicase bound to single-stranded DNA and ADP
8. Crystal structure of a human alkylbase-DNA repair enzyme complexed to DNA: Mechanisms for nucleotide flipping and base excision
9. Active site of (A)BC excinuclease. I. Evidence for 5′ incision by UvrC through a catalytic site involving Asp399, Asp438, Asp466 and His538 residues
10. Crystal structure of the DNA nucleotide excision repair enzyme UvrB from Thermus thermophilus
11. Dimerization of Escherichia coli UvrA and its binding to undamaged and ultraviolet-light damaged DNA
12. Helicase motifs V and VI of the Escherichia coli UvrB protein of the UvrABC endonuclease are essential for the formation of the preincision complex
13. Characterisation of the Escherichia coli damage-independent UvrBC endonuclease activity
14. The effect of the DNA flanking the lesion on formation of the UvrB-DNA preincision complex. Mechanism for the UvrA-mediated loading of UvrB onto the damage
15. The role of ATP binding and hydrolysis by UvrB during nucleotide excision repair
16. Role of the Escherichia coli nucleotide excision repair proteins in DNA replication
17. Crystal structure of Thermus thermophilus HB8 UvrB protein, a key enzyme of nucleotide excision repair
18. Helicase properties of the Escherichia coli UvrAB protein complex
19. Characterization of the helicase activity of the Escherichia coli UvrAB protein complex
20. ATPase activity of the UvrA and UvrB protein complexes of the Escherichia coli UvrABC endonuclease
21. The (A)BC excinuclease of Escherichia coli has only the UvrB and UvrC subunits in the incision complex
22. Escherichia coli DNA photolyase stimulates UvrABC excision nuclease in vitro
23. Purification and properties of the UvrA protein from Escherichia coli
24. The role of Escherichia coli UvrB in nucleotide excision repair
25. A nucleotide-flipping mechanism from the structure of human uracil-glycosylase bound to DNA
26. Crystal structure of UvrB, a helicase adapted for nucleotide excision repair
27. Both ATPase sites of Escherichia coli UvrA have functional roles in nucleotide excision repair
28. Evidence for dinucleotide flipping by DNA photolyase
29. Mechanism of action of the Escherichia coli UvrABC nuclease: Clues to the damage recognition problem
30. Crystal structures of complexes of PcrA DNA helicase with a DNA substrate indicate an inchworm mechanism
31. Architecture of nucleotide excision repair complexes: DNA is wrapped by UvrB before and after damage recognition
32. Analysis of UvrABC endonuclease reaction intermediates on cisplatin-damaged DNA using mobility shift gel electrophoresis
33. Protein complexes formed during the incision reaction catalyzed by the Escherichia coli UvrABC endonuclease