Generation and refinement of peptide mimetic ligands for paratope-specific purification of monoclonal antibodies

Analytical Biochemistry

Volumn 296, Issue 1, 2001, Pages 9-17

  Murray, Andrea ^a   Smith, Richard G ^a   Brady, Kevin ^a   Williams, Samantha ^b   Badley, R Andrew ^a   Price, Michael R ^a  

^a UNIVERSITY OF NOTTINGHAM   (United Kingdom)

^b UNILEVER RESEARCH   (United Kingdom)

Author keywords

Affinity chromatography; Mimotope; Monoclonal antibody; Phage display; Replacement net analysis

Indexed keywords

AFFINITY CHROMATOGRAPHY; ANTIGENS; CRYSTAL STRUCTURE; LIGANDS; MONOCLONAL ANTIBODIES; PHAGE DISPLAY; PURIFICATION;

AFFINITY MATURATION; ANTIBODY PURIFICATION; CHEMICAL LIBRARIES; CONVENTIONAL METHODS; IMMUNOREACTIVITIES; MIMOTOPE; PHAGE DISPLAY LIBRARIES; REPLACEMENT NET ANALYSIS;

PEPTIDES;

AMINO ACID; ESTRONE GLUCURONIDE; LIGAND; MONOCLONAL ANTIBODY; PEPTIDE;

AFFINITY CHROMATOGRAPHY; AMINO ACID SEQUENCE; ANTIBODY COMBINING SITE; ANTIGEN BINDING; ARTICLE; BINDING AFFINITY; CRYSTAL STRUCTURE; IMMUNOREACTIVITY; LIBRARY; LIGAND BINDING; METHODOLOGY; MODEL; PHAGE DISPLAY; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN LOCALIZATION; PROTEIN STRUCTURE; PROTEIN SYNTHESIS; PURIFICATION;

EID: 0035450523     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.2001.5235     Document Type: Article

References (35)

1. Purification of anti-epithelial mucin monoclonal antibodies by epitope affinity chromatography
2. Purification of monoclonal antibodies by epitope and mimotope affinity chromatography
3. Design of ligands for the purification of anti-MUC1 antibodies by peptide epitope affinity chromatography
4. Characterization of a recombinant Fv fragment of anti-MUC1 antibody HMFG1
5. Production and characterization of a recombinant anti-MUC1 scFv reactive with human carcinomas
6. Production and characterization of an anti-(MUC1 mucin) recombinant diabody
7. Construction of a reshaped HMFG1 antibody and comparison of its fine specificity with that of the parent mouse antibody
8. Targeting superficial bladder cancer by the intravesical administration of copper-67 labeled anti-MUC1 mucin antibody C595
9. Monoclonal antibodies against progesterone: Effect of steroid-carrier coupling position on antibody affinity
10. Identifying the fertile phase of the human menstrual cycle
11. Temporal relationships between ovulation and defined changes in the concentration of the plasma estradiol-17B, lutenising hormone, follicle stimulating hormone and progesterone. I. Probit analysis
12. Monitoring reproductive hormones to detect the fertile period: Development of Persona - The first home use system
13. Purification of an Fv antibody fragment using two antigen analogues: One as a column ligand, the other as a specific eluant
14. Filamentous fusion phage: Novel expression vectors that display cloned antigens on the virion surface
15. Mimotopes of polyreactive anti-DNA antibodies identified using phage-display peptide libraries
16. Peptide mimicry of carbohydrate epitopes on human immunodeficiency virus
17. Peptide mimotopes of carbohydrate antigens
18. Epitope discovery using peptide libraries displayed on phage
19. Recognition by human sera and immunogenicity of HbsAg mimotopes selected from and M14 phage display library
20. A general strategy to identify mimotopes of pathological antigens using only random peptides and human sera
21. Reproducing the immune response against the Plasmodium vivax merozoite surface protein 1 with mimotpes selected from a phage-displayed peptide library
22. Continuous cultures of fused cells secreting antibody of predefined specificity
23. C595 - A monoclonal antibody against the protein core of human urinary epithelial mucin commonly expressed in breast carcinomas
24. Selection of antibody ligands from a large library of oligopeptides expressed on a multivalent exposition vector
25. Mimicking of discontinuous epitopes by phage-displayed peptides. I. Epitope mapping of human H ferritin using a phage library of constrained peptides
26. Identification of epitopes within beta lactoglobulin recognized by polyclonal antibodies using phage display and PEPSCAN
27. Strategies for epitope design using peptide synthesis
28. Antibody fragment Fv4155 bound to two closely related steroid hormones: The structural basis of fine specificity
29. Protein A mimetic peptide ligand for affinity purification of antibodies
30. Design synthesis and application of a protein A mimetic
31. Affinity purification of proteins using ligands derived from peptide libraries
32. Epitope mapping of anti-MUC1 mucin core monoclonal antibodies
33. Identification of D-peptide ligands through mirror-image phage display
34. Inhibiting HIV-1 entry: Discovery of d-peptide inhibitors that target the gp41 coiled-coil pocket
35. Affinity purification of mouse monoclonal IgE using a protein A mimetic ligand (TG19318) immobilized on solid supports