Affinity purification of mouse monoclonal IgE using a protein A mimetic ligand (TG19318) immobilized on solid supports

Journal of Molecular Recognition

Volumn 11, Issue 1-6, 1998, Pages 247-249

  Palombo, Giovanna ^a   Rossi, Maria ^a   Cassani, Giovanni ^a   Fassina, Giorgio ^a  

^a TECNOGEN SCpA   (Italy)

Author keywords

IgE purification; Mimetic ligand; Protein A

Indexed keywords

AFFINITY CHROMATOGRAPHY; COMBINATORIAL LIBRARY; IMMUNOASSAY; IMMUNOGLOBULIN E; PEPTIDE LIBRARY; PROTEIN PURIFICATION; SDS POLYACRYLAMIDE GEL ELECTROPHORESIS; SODIUM DIHYDROGEN PHOSPHATE;

EID: 0032448774     PISSN: 09523499     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1099-1352(199812)11:1/6<247::AID-JMR432>3.0.CO;2-L     Document Type: Conference Paper

References (6)

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2. Ikeyama, S., Nakagawa, S., Arakawa, M., Sugino, H. and Kakinuma, A. (1986). Purification and characterization of IgE produced by human myeloma cell line, U266. Mol. Immunol. 23, 159-167.
3. Lehrer, S. B. (1979). Isolation of IgE from normal mouse serum. Immunology 36, 103-109.
4. Nagy, E., Berczi, I. and Sehon, A. H. (1991). Growth inhibition of murine mammary carcinoma by monoclonal IgE antibodies specific for the mammary tumor virus. Cancer Immunol. Immunother. 34, 63-69.
5. Phillips, T. M., More, N. S., Queen, W. D. and Thompson, A. M. (1985). Isolation and quantitation of serum IgE levels by high-performance immunoaffinity chromatography. J. Chromatogr. 327, 205-211.
6. Zola, H., Garland, L. G., Cox, H. C. and Adcock, J. J. (1978). Separation of IgE from IgG subclasses using staphylococcal protein A. Int. Arch. Allergy Appl. Immunol. 56, 123-127.