Optimisation of retroviral supernatant production conditions for the genetic modification of human CD34+ cells

Journal of Gene Medicine

Volumn 3, Issue 3, 2001, Pages 219-227

  Dando, Jonathan S ^a   Aiuti, Alessandro ^a   Deola, Sara ^a   Ficara, Francesca ^a   Bordignon, Claudio ^a  

^a SAN RAFFAELE SCIENTIFIC INSTITUTE   (Italy)

Author keywords

CD34+ cells; Quality control; Retroviral supernatant

Indexed keywords

MURINAE;

CD34 ANTIGEN; GREEN FLUORESCENT PROTEIN; PHOTOPROTEIN;

ANIMAL; ARTICLE; BIOSYNTHESIS; CELL CULTURE; CELL STRAIN 3T3; GENE TRANSFER; GENE VECTOR; GENETIC TRANSDUCTION; GENETIC TRANSFECTION; GENETICS; HEMATOPOIETIC STEM CELL; HUMAN; IMMUNOLOGY; METABOLISM; METHODOLOGY; MOUSE; PHYSIOLOGY; RETROVIRUS; VIRUS GENE;

3T3 CELLS; ANIMALS; ANTIGENS, CD34; CELLS, CULTURED; GENE TRANSFER TECHNIQUES; GENES, VIRAL; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; HEMATOPOIETIC STEM CELLS; HUMANS; LUMINESCENT PROTEINS; MICE; RETROVIRIDAE; TRANSDUCTION, GENETIC; TRANSFECTION;

EID: 0035351101     PISSN: 1099498X     EISSN: None     Source Type: Journal    
DOI: 10.1002/1521-2254(200105/06)3:3<219::AID-JGM184>3.0.CO;2-E     Document Type: Article

References (24)

1. Nolta JA, Smogorzewska EM, Kohn DB. Analysis of optimal conditions for retroviral-mediated transduction of primitive human hematopoietic cells. Blood 1995; 86: 101-110.
2. Shimakura Y, Kawada H, Ando K, et al. Murine stromal cell line HESS-5 maintains reconstituting ability of ex vivo-generated hematopoietic stem cells from human bone marrow and cytokine-mobilized peripheral blood. Stem Cells 2000; 18: 183-189.
3. + cells into rapid division. Exp Hematol 1999; 27: 1019-1028.
4. Blau CA, Neff T, Papayannopoulou T. Cytokine prestimulation as a gene therapy strategy: implications for using the MDR1 gene as a dominant selectable marker. Blood 1997; 89: 146-154.
5. + progenitors to sustain long-term hematopoiesis in immune-deficient mice after ex vivo retroviral-mediated transduction. Blood 1997; 89: 446-456.
6. Hennemann B, Conneally E, Pawliuk R, et al. Optimisation of retroviral-mediated gene transfer to human NOD/SCID mouse repopulating cord blood cells through systematic analysis of protocol variables. Exp Hematol 1999; 27: 817-825.
7. + hematopoietic cells. Hum Gene Ther 1997; 8: 2193-2206.
8. Kotani H, Newton III PB, Zhang S, et al. Improved methods for retroviral vector transduction and production for gene therapy. Hum Gene Ther 1994; 5: 19-28.
9. Forestell SP, Bohnlein E, Rigg RJ. Retroviral endpoint titer is not predictive of gene transfer efficiency: implications for vector production. Gene Ther 1995; 2: 723-730.
10. Kaptein LC, Greijer AE, Valerio D, van Beusechem VW. Optimised conditions for the production of recombinant amphotropic retroviral vector preparations. Gene Ther 1997; 4: 172-176.
11. Sandstrom CE, Collins PC, McAdams TA, et al. Comparison of whole serum-deprived media for ex vivo expansion of hematopoietic progenitor cells from cord blood and mobilized peripheral blood mononuclear cells. J Hematother 1996; 5: 461-473.
12. Sekhar M, Kotani H, Doren S, et al. Retroviral transduction of CD34-enriched hematopoietic progenitor cells under serum-free conditions. Hum Gene Ther 1996; 7: 33-38.
13. + cord blood cells under serum-free conditions. Hum Gene Ther 1997; 8: 1651-1658.
14. Glimm H, Flugge K, Mobest D, et al. Efficient serum-free retroviral gene transfer into primitive human hematopoietic progenitor cells by a defined high titre, non concentrated vector-containing media. Hum Gene Ther 1998; 9: 771-778.
15. Conneally E, Eaves CJ, Humphries RK. Efficient retroviral-mediated gene transfer to human cord blood stem cells with in vivo repopulating potential. Blood 1998; 91: 3487-3493.
16. Schilz AJ, Brouns G, Knoss H, et al. High efficiency gene transfer to human hematopoietic SCID-repopulating cells under serum-free conditions. Blood 1998; 92: 3163-3171.
17. Miller AD, Buttimore C. Redesign of retrovirus packaging cell lines to avoid recombination leading to helper virus production. Mol Cell Biol 1986; 6: 2895-2902.
18. Markowitz D, Goff S, Bank A. Construction of safe and efficient amphotropic packaging cell lines. Virology 1988; 167: 400-406.
19. Miller AD, Rosman GJ. Improved retroviral vectors for gene transfer and expression. Biotechniques 1989; 7: 980-990.
20. Emery DW, Andrews RG, Papayannopoulou T. Differences among nonhuman primates in susceptibility to bone marrow progenitor transduction with retrovirus vectors. Gene Ther 2000; 7: 359-367.
21. McAdams TA, Miller WM, Papoutsakis ET. Variations in culture pH affect the cloning efficiency and differentiation of progenitor cells in ex vivo hematopoiesis. Br J Haematol 1997; 97: 889-895.
22. Cashman JD, Clark-Lewis I, Eaves AC, Eaves CJ. Differentiation stage-specific regulation of primitive human hematopoietic progenitor cycling by exogenous and endogenous inhibitors in an in vivo model. Blood 1999; 94: 3722-3729.
23. Hogge DE, Lansdorp PM, Reid D, Gerhard B, Eaves CJ. Enhanced detection, maintenance, and differentiation of primitive human hematopoietic cells in cultures containing murine fibroblasts engineered to produce human steel factor, interleukin-3, and granulocyte colony-stimulating factor. Blood 1996; 88: 3765-3773.
24. Aiuti A, Friedrich C, Sieff CA, Gutierrez-Ramos JC. Identification of distinct elements of the stromal microenvironment that control human hematopoietic stem/progenitor cell growth and differentiation. Exp Hematol 1998; 26: 143-157.