Green fluorescent protein as a novel tool to measure apoptosis and necrosis

Cytometry

Volumn 43, Issue 2, 2001, Pages 126-133

  Strebel, Alessandro ^a   Harr, Thomas ^a   Bachmann, Felix ^a   Wernli, Marion ^a   Erb, Peter ^a  

^a UNIVERSITY OF BASEL   (Switzerland)

Author keywords

Annexin V; Apoptosis; FasL; Flow cytometry; Green fluorescent protein (GFP); Interleukin 3 (IL 3); Necrosis; Propidium iodide (PI); Tumor necrosis factor related apoptosis inducing ligand (TRAIL)

Indexed keywords

CASPASE INHIBITOR; COMPLEMENT; FAS LIGAND; GREEN FLUORESCENT PROTEIN; INTERLEUKIN 3; POLYCLONAL ANTIBODY; TUMOR NECROSIS FACTOR RELATED APOPTOSIS INDUCING LIGAND; APOPTOSIS REGULATORY PROTEIN; DYES, REAGENTS, INDICATORS, MARKERS AND BUFFERS; FAS ANTIGEN; FASL PROTEIN, MOUSE; FASLG PROTEIN, HUMAN; LIGAND; MEMBRANE PROTEIN; PHOTOPROTEIN; TNFSF10 PROTEIN, HUMAN; TNFSF10 PROTEIN, MOUSE; TUMOR NECROSIS FACTOR ALPHA;

ANIMAL CELL; APOPTOSIS; ARTICLE; ASSAY; CELL DEATH; CONTROLLED STUDY; FLUORESCENCE; HUMAN; HUMAN CELL; INTERMETHOD COMPARISON; MOUSE; NONHUMAN; PRIORITY JOURNAL; SCREENING; STAINING; ANIMAL; BAGG ALBINO MOUSE; CELL CULTURE; CELL LINE; DBA MOUSE; FLOW CYTOMETRY; GENETIC TRANSFECTION; GENETICS; LEUKEMIA CELL LINE; METABOLISM; METHODOLOGY; NECROSIS; PATHOLOGY; PHYSIOLOGY; SOLUBILITY;

ANIMALS; ANTIGENS, CD95; APOPTOSIS; APOPTOSIS REGULATORY PROTEINS; CELL LINE; FAS LIGAND PROTEIN; FLOW CYTOMETRY; GREEN FLUORESCENT PROTEINS; HUMANS; INDICATORS AND REAGENTS; INTERLEUKIN-3; JURKAT CELLS; LIGANDS; LUMINESCENT PROTEINS; MEMBRANE GLYCOPROTEINS; MICE; MICE, INBRED BALB C; MICE, INBRED DBA; NECROSIS; SOLUBILITY; TNF-RELATED APOPTOSIS-INDUCING LIGAND; TRANSFECTION; TUMOR CELLS, CULTURED; TUMOR NECROSIS FACTOR-ALPHA;

EID: 0035254495     PISSN: 01964763     EISSN: None     Source Type: Journal    
DOI: 10.1002/1097-0320(20010201)43:2<126::AID-CYTO1027>3.0.CO;2-J     Document Type: Article

References (26)

1. The central effectors of cell death in the immune system
2. Apoptosis in the pathogenesis and treatment of disease
3. Cell death: The significance of apoptosis
4. Apoptosis: Mechanisms and roles in pathology
5. Glucocorticoid-induced thymocyte apoptosis is associated with endogenous endonuclease activation
6. The topoisomerase II inhibitor teniposide (VM-26) induces apoptosis in unstimulated mature murine lymphocytes
7. Apoptosis: The biochemistry and molecular biology of programmed cell death
8. Apoptosis and programmed cell death in health and disease
9. A rapid and simple method for measuring thymocyte apoptosis by propidium iodide staining and flow cytometry
10. Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation
11. A novel assay for apoptosis. Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin V
12. pEF-BOS, a powerful mammalian expression vector
13. Construction and applications of a highly transmissible murine retrovirus shuttle vector
14. Spontaneous, in vitro, malignant transformation of a basophil/mast cell line
15. Apoptosis in hematopoietic cells (FL5.12) caused by interleukin-3 withdrawal: Relationship to caspase activity and the loss of glutathione
16. Green fluorescent protein as a marker for gene expression
17. Implications for bcd mRNA localization from spatial distribution of exu protein in Drosophila oogenesis
18. A rapid, quantitative and inexpensive method for detecting apoptosis by flow cytometry in transiently transfected cells
19. Use of green fluorescent protein in studies of apoptosis of transfected cells
20. Isolation and characterization of the human chromosomal gene for polypeptide chain elongation factor-1 alpha
21. Structural and spectral response of green fluorescent protein variants to changes in pH
22. Green fluorescent protein as a noninvasive intracellular pH indicator
23. Measurement of cytosolic, mitochondrial, and Golgi pH in single living cells with green fluorescent proteins
24. pH-dependent fluorescence of a heterologously expressed Aequorea green fluorescent protein mutant: In situ spectral characteristics and applicability to intracellular pH estimation
25. Apoptosis in C3H-10T1/2 cells: Roles of intracellular pH, protein kinase C, and the Na+/H+ antiporter
26. Assays for DNA fragmentation, endonucleases, and intracellular pH and Ca2+ associated with apoptosis