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In earlier studies of covalently attached polymers on silanized glass substrates, the rupture of the Si-C bond in the silane anchor determined the available force range. There, an average rupture force of ∼2 nN was observed (cf. ref 30). Here, the β-hydroxyalkylamino groups formed upon reaction of amino groups with the epoxysurface functions appears to be the limiting factor. On average, the investigated system holds forces of ∼1.3 nN, the highest observed rupture force of single polymer strands was ∼1.9 nN (compare Figure 4a).
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For comparison, the freely jointed chain (FJC) model has been used but was found to model the experimental data with less accuracy.
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