Isolation and characterisation of five neurotoxic and cardiotoxic polypeptides from the sea anemone Anthopleura elegantissima

Toxicon

Volumn 39, Issue 5, 2001, Pages 693-702

  Bruhn, T ^a   Schaller, C ^b   Schulze, C ^b   Sanchez Rodriguez, J ^c   Dannmeier, C ^a   Ravens, U ^d   Heubach, J F ^d   Eckhardt, K ^d   Schmidtmayer, J ^e   Schmidt, H ^e   Aneiros, A ^f   Wachter, E ^g   Beress L ^a  

^a Kiel and Lungen Clinic Grosshansdorf   (Germany)

^b UNIVERSITY MEDICAL CENTER HAMBURG EPPENDORF   (Germany)

^c UNIVERSIDAD NACIONAL AUTÓNOMA DE MÉXICO   (Mexico)

^d DRESDEN UNIVERSITY OF TECHNOLOGY   (Germany)

^e UNIVERSITY OF KIEL   (Germany)

^f Marine Bioactive Department and Natural Products   (Cuba)

^g UNIVERSITY OF MUNICH   (Germany)

Author keywords

Anthopleura elegantissima; Carcinus maenas; Cardiotoxicity; Neurotoxicity; Polypeptide toxins

Indexed keywords

POLYPEPTIDE; SEA ANEMONE TOXIN;

ANION EXCHANGE; ARTICLE; CARDIOTOXICITY; CHRONOTROPISM; CONTROLLED STUDY; CRAB; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; INOTROPISM; ISOLATION PROCEDURE; LD 50; NEUROTOXICITY; NONHUMAN; PRIORITY JOURNAL; PROTEIN PURIFICATION; SEA ANEMONE; TOXIN ANALYSIS;

ANIMALS; CELLS, CULTURED; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CNIDARIAN VENOMS; GUINEA PIGS; LETHAL DOSE 50; MALE; MICE; MYOCARDIAL CONTRACTION; NEUROTOXINS; PATCH-CLAMP TECHNIQUES; PEPTIDES; SEA ANEMONES; SODIUM CHANNELS;

ACTINIARIA; ALOCASIA MACRORRHIZOS; ANEMONE; ANEMONIA SULCATA; ANTHOPLEURA ELEGANTISSIMA; CARCINUS MAENAS; CAVIA PORCELLUS; DECAPODA (CRUSTACEA); GRAPSIDAE; MAMMALIA; SUS SCROFA;

EID: 0035238877     PISSN: 00410101     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0041-0101(00)00199-9     Document Type: Article

References (35)

1. Cardiotoxic effect of two toxins isolated from the sea anemone
2. The action of a toxin from the sea anemone Anemonia sulcata upon mammalian heart muscles
3. Differences in the binding sites of two site-3 sodium channel toxins
4. Reinigung zweier krabbenlahmender Toxine aus der Seeanemone Anemonia sulcata
5. Purification of three polypeptides with neuro- and cardio-toxic activity from the sea anemone Anemonia sulcata
6. + channels: isolation and characterization
7. Decreased rate of sodium conductance inactivation in the mode of Ranvier induced by polypeptide toxin from sea anemone
8. Sea anemone toxin and scorpion toxin share a common receptor site associated with the action potential sodium ionophore
9. Isolation of Leu-Pro-Pro-Gly-Pro-Leu-Pro-Arg-Pro-NH2 (Antho-RPamide), an N-terminally protected, biologically active neuropeptide from sea anemones
10. Cardiac stimulatory, cytotoxic and cytolytic activity of extracts of sea anemones
11. Conductance of the sodium channel in myelinated nerve fibres with modified sodium activation
12. The role of exposed tryptophan residues in the activity of the cardiotonic polypeptide anthopleurin B
13. Dose-dependent modulation of the cardiac sodium channel by sea anemone toxin ATXII
14. Contrast between solitary and clonal lifestyles in the sea anemone Anthopleura elegantissima
15. Importance of the unique cationic residues arginine 12 and lysine 49 in the activity of the cardiotonic polypeptide anthopleurin B
16. Swiss-Model and the Swiss-PdbViewer: an environment for comparative protein modeling
17. Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches
18. Effects of ATX II on intracellular sodium and contractility in rat and guinea-pig myocardium
19. Cardioactive substances that prolong the open state of sodium channels
20. Identification and characterization of novel sodium channel toxins from the sea anemone Anthopleura xanthogrammica
21. Multiple cationic residues of anthopleurin B that determine high affinity and channel isoform discrimination
22. Importance of highly conserved anionic residues and electrostatic interactions in the activity and structure of the cardiotonic polypeptide anthopleurin B
23. Positively charged amino acid residues located similarly in sea anemone and scorpion toxins
24. Anthopleurin A, B and C cardiotonic polypeptides from the sea anemones Anthopleura xanthogramica (Brandt) and Anthopleura elegantissima (Brandt)
25. Three-dimensional structure in solution of the polypeptide cardiac stimulant anthopleurin-A
26. Protein modelling by E-mail
27. ProMod and Swiss Model: Internet-based tools for automated comparative protein modelling
28. Selective effects of disturbance on populations of sea anemones from northern Baja California, Mexico
29. Electromechanical studies of an Anemonia sulcata toxin in mammalian cardiac muscle
30. + channel alpha subunit
31. Sea anemone toxin: a tool to study molecular mechanisms of nerve conduction and exhibition secretion coupling
32. A simple biochemical method for the search for bioactive polypeptides in a sea anemone (Anemonia sulcata)
33. Isolation and amino acid sequence of a morphogenetic peptide from hydra
34. Isolation of isotoxins from the sea anemone Anemonia sulcata by HPLC chromatography