125I-Antisauvagine-30: A novel and specific high-affinity radioligand for the characterization of corticotropin-releasing factor type 2 receptors

Neuropharmacology

Volumn 40, Issue 1, 2001, Pages 114-122

  Higelin, Jacqueline ^a   Py Lang, Gabrielle ^a   Paternoster, Cristina ^b   Ellis, Gareth J ^b   Patel, Arvind ^b   Dautzenberg, Frank M ^a  

^a F HOFFMANN LA ROCHE LTD   (Switzerland)

^b GENERAL ELECTRIC COMPANY   (United States)

Author keywords

G protein coupled receptor; Ligand binding; Ligand selectivity; Receptor expression

Indexed keywords

CORTICOTROPIN RELEASING FACTOR; IODINE 125; RECEPTOR SUBTYPE; SAUVAGINE; ANTISAUVAGINE 30; CORTICOTROPIN RELEASING FACTOR RECEPTOR; CRF RECEPTOR TYPE 2; DIAGNOSTIC AGENT; DYES, REAGENTS, INDICATORS, MARKERS AND BUFFERS; GUANINE NUCLEOTIDE; PEPTIDE; PEPTIDE FRAGMENT; RADIOPHARMACEUTICAL AGENT;

ANIMAL TISSUE; ARTICLE; AUTORADIOGRAPHY; BRAIN SLICE; CELL POPULATION; HORMONE RESPONSE; LABELING INDEX; LIGAND BINDING; NONHUMAN; PRIORITY JOURNAL; RAT; RECEPTOR AFFINITY; RECEPTOR BINDING; SEQUENCE HOMOLOGY; TISSUE DISTRIBUTION; ANIMAL; BINDING COMPETITION; BRAIN LEVEL; CELL CULTURE; DRUG EFFECT; HUMAN; IN VITRO STUDY; KINETICS; MEMBRANE; METABOLISM; RADIOASSAY; XENOPUS;

ANIMALS; BINDING, COMPETITIVE; BRAIN CHEMISTRY; CELLS, CULTURED; CORTICOTROPIN-RELEASING HORMONE; GUANINE NUCLEOTIDES; HUMANS; INDICATORS AND REAGENTS; KINETICS; MEMBRANES; PEPTIDE FRAGMENTS; PEPTIDES; RADIOLIGAND ASSAY; RADIOPHARMACEUTICALS; RATS; RECEPTORS, CORTICOTROPIN-RELEASING HORMONE; XENOPUS;

EID: 0035219763     PISSN: 00283908     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0028-3908(00)00105-2     Document Type: Article

References (32)

1. The role of corticotropin-releasing factor in depression and anxiety disorders
2. 2 α and β splice variants: Pharmacological characterization using radioligand binding and a luciferase gene expression assay
3. G proteins in signal transduction
4. 1 receptor mRNA expression
5. Corticotropin-releasing factor receptors: From molecular biology to drug design
6. Identification of two corticotropin-releasing factor receptors with high ligand selectivity from Xenopus laevis: Unusual pharmacology of the type 1 receptor
7. Mapping of the ligand-selective domain of the Xenopus laevis corticotropin-releasing factor receptor 1: Implications for the ligand-binding site
8. The ligand-selective domains of corticotropin-releasing factor type 1 and type 2 receptors reside in different extracellular domains: Generation of chimeric receptors with a novel ligand-selective profile
9. N-Terminal splice variants of the type I PACAP receptor: Isolation, characterization, and ligand binding/selectivity determinants
10. Functional characterization of corticotropin-releasing factor type 1 receptor endogenously expressed in human embryonic kidney 293 cells
11. Evidence for the abundant expression of Arginine 185 containing human CRF(2α) receptors and the role of position 185 for receptor-ligand selectivity
12. Cloning and characterization of human urocortin
13. Physiological and behavioral responses of corticotropin releasing factor administration: Is CRF a mediator of anxiety or stress responses?
14. 3H]-urocortin
15. 0-Sauvagine: A novel high affinity radioligand for the pharmacological and biochemical study of human corticotropin-releasing factor(2α) receptors
16. Potent, structurally constrained agonists and competitive antagonists of corticotropin-releasing factor
17. Regulation of the stress response by corticotropin-releasing factor receptors
18. The rationale for corticotropin-releasing hormone receptor (CRH-R) antagonists to treat depression and anxiety
19. G-protein coupled receptors - The splicing on the cake
20. Urocortin, a novel neuropeptide with anxiogenic-like properties
21. Corticotropin-releasing factor receptor type 1 from Tupaia belangeri: Cloning, functional expression and tissue distribution
22. Isolation and pharmacological characterization of two functional splice variants of corticotropin-releasing factor type 2 receptor from Tupaia belangeri
23. Comparison of an agonist, urocortin, and an antagonist, astressin, as radioligands for characterization of corticotropin-releasing factor receptors
24. Structural requirements for peptidic antagonists of the corticotropin-releasing factor receptor (CRFR): Development of CRFR2β-selective antisauvagine-30
25. Autoradiographic and in situ hybridization localization of corticotropin-releasing factor 1 and 2 receptors in nonhuman primate brain
26. Radiolabelling of the human 5-HT2: A receptor with an agonist, a partial agonist and an antagonist: Effects on apparent agonist affinities
27. Expression and characterisation of human and rat CRF(2α) receptors
28. Scintillation proximity assay: A sensitive and continuous isotopic method for monitoring ligand/receptor and antigen/antibody interactions
29. Corticotropin-releasing factor (CRF) family of ligands and their receptors
30. Urocortin, a mamalian neuropeptide related to fish urotensin I and to corticotropin-releasing factor
31. Identification of amino acids in the N-terminal domain of corticotropin-releasing factor receptor 1 that are important determinants of high affinity ligand binding
32. Brain and pituitary receptors for corticotropin releasing factor: Localization and differential regulation after adrenalectomy