Application of high-density array-based signature-tagged mutagenesis to discover novel Yersinia virulence-associated genes

Infection and Immunity

Volumn 69, Issue 12, 2001, Pages 7810-7819

  Karlyshev, A V ^a   Oyston, P C F ^a   Williams, K ^a   Clark, G C ^a   Titball, R W ^a   Winzeler, E A ^a   Wren, B W ^a  

^a LONDON SCHOOL OF HYGIENE AND TROPICAL MEDICINE   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

DNA;

ARTICLE; BACTERIAL VIRULENCE; BIOSYNTHESIS; GENE CONTROL; GENE SEQUENCE; HYBRIDIZATION; MUTAGENESIS; NONHUMAN; NUCLEOTIDE SEQUENCE; PATHOGENESIS; PATHOGENICITY; PRIORITY JOURNAL; TRANSPOSON; YERSINIA; YERSINIA PESTIS; YERSINIA PSEUDOTUBERCULOSIS; YERSINIOSIS;

ANIMALS; BACTERIAL OUTER MEMBRANE PROTEINS; DNA TRANSPOSABLE ELEMENTS; GENES, BACTERIAL; LETHAL DOSE 50; LIPOPOLYSACCHARIDES; MICE; MOLECULAR SEQUENCE DATA; MUTAGENESIS, INSERTIONAL; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS; PHOSPHOLIPASES A; VIRULENCE; YERSINIA PSEUDOTUBERCULOSIS; YERSINIA PSEUDOTUBERCULOSIS INFECTIONS;

EID: 0035189384     PISSN: 00199567     EISSN: None     Source Type: Journal    
DOI: 10.1128/IAI.69.12.7810-7819.2001     Document Type: Article

References (56)

1. Yersinia pestis, the cause of plague, is a recently emerged clone of Yersinia pseudotuberculosis
2. Lipopolysaccharide O side chain of Yersinia enterocolitica O:3 is an essential virulence factor in an orally infected murine model
3. Escherichia coli outer membrane phospholipase A: Role of two serines in enzymatic activity
4. Sequence comparison of outer membrane phospholipases A: Implications for structure and for the catalytic mechanism
5. Molecular characterization of enterobacterial pldA genes encoding outer membrane phospholipase A
6. Factors promoting acute and chronic diseases caused by yersiniae
7. Identification of a virulence gene cluster of Mycobacterium tuberculosis by signature-tagged transposon mutagenesis
8. Type III machines of Gram-negative bacteria: Delivering the goods
9. Use of signature-tagged transposon mutagenesis to identify Vibrio cholerae genes critical for colonization
10. Identification of a hotspot for transformation of Neisseria meningitidis by shuttle mutagenesis using signature-tagged transposons
11. Staphylococcus aureus genetic loci impacting growth and survival in multiple infection environments
12. Complex lipid determines tissue-specific replication of Mycobacterium tuberculosis in mice
13. Identification of Yersinia enterocolitica genes affecting survival in an animal host using signature-tagged transposon mutagenesis
14. Mini-Tn5 transposon derivatives for insertion mutagenesis, promoter probing, and chromosomal insertion of cloned DNA in gram-negative eubacteria
15. Nature of Escherichia coli mutants deficient in detergent-resistant and/or detergent-sensitive phospholipase A
16. Characterization of Helicobacterpylori PldA, a phospholipase with a role in colonization of the gastric mucosa
17. Discovery of virulence genes of Legionclla pneumophila by using signature tagged mutagenesis in guinea pig pneumonia model
18. Acute mesenteric lymphadenitis due to Yersinia pseudotuberculosis lacking a virulence plasmid
19. A broad-host-range cloning vector transposable to various replicons
20. Simultaneous identification of bacterial virulence genes by negative selection
21. Transposon vectors containing non-antibiotic resistance selection markers for cloning and stable chromosomal insertion of foreign genes in gram-negative bacteria
22. Signature tagged mutagenesis
23. YopK, a novel virulence determinant of Yersinia pseudotuberculosis
24. Identification of genes required for chronic persistence of Brucella abortus in mice
25. Identification of Streptococcus agalactiae virulence genes in the neonatal rat sepsis model using signature-tagged mutagenesis
26. Single-primer PCR procedure for rapid identification of transposon insertion sites
27. An automated multiplex oligonucleotide synthesizer: Development of high-throughput, low-cost DNA synthesis. Detection of genes essential in specific niches by signature-tagged mutagenesis
28. Identification and characterization of in vivo attenuated mutants of Brucella melitensis
29. Identification of Staphylococcus aureus virulence genes in a murine model of bacteraemia using signature-tagged mutagenesis
30. Conditionally replicative and conjugative plasmids carrving lacZα for cloning, mutagenesis, and allele replacement in bacteria
31. An aroA mutant of Yersinia pestis is attenuated in guinea-pigs, but virulent in mice
32. Signal transduction and invasion of epithelial cells by S. typhimurium
33. Signature-tagged mutagenesis and the hunt for virulence factors
34. Large-scale identification of virulence genes from Streptococcus pneumoniae
35. Characterisation of the lipopolysaccharide of Yersinia pestis
36. A simple method for estimating fifty percent endpoints
37. Role of O-antigen variation in the immune response
38. Detection of pathogenic Yersinia enterocolitica by using congo-red magnesium oxalate agar medium
39. Increased virulence of Yersinia pseudotuberculosis by two independent mutations
40. Phospholipase A production by isolates of Salmonella species
41. Microarrays: Biotechnology's discovery platform for functional genomics
42. Phospholipase A of Yersinia enterocolitica contributes to pathogenesis in a mouse model
43. Phospholipase A2: From basic research to clinical reality
44. Identification and characterization of the PutP proline permease that contributes to in vivo survival of Staphylococcus aureus in animal models
45. A method for construction of bacterial hosts for lac-based cloning and expression vectors: Alpha-complementation and regulated expression
46. Identification of a virulence locus encoding a second type III secretion system in Salmonella typhimurium
47. Quantitative phenotypic analysis of yeast deletion mutants using a highly parallel molecular bar-coding strategy
48. 6
49. Characterization of the O-antigen gene clusters of Yersinia pseudotuberculosis and the cryptic O-antigen gene cluster of Yersinia pestis shows that the plague bacillus is most closely related to and has evolved from Y. pseudotuberculosis serotype O:1b
50. Bacterial phospholipases
51. Y phenotypes of yersiniae
52. Functional characteristics of the S. cerevisiae genome by gene deletion and parallel analysis
53. Genome-wide expression monitoring in Saccharomyces cerevisiae
54. Identification of protease and rpoN-associated genes of uropahogenic Proteus mirabilis by negative selection on a mouse model of ascending urinary tract infection