Complex lipid determines tissue-specific replication of Mycobacterium tuberculosis in mice

Nature

Volumn 402, Issue 6757, 1999, Pages 79-83

  Cox, Jeffery S ^a   Chess, Bing ^a   McNeil, Michael ^b   Jacobs Jr , William R ^a  

^a ALBERT EINSTEIN COLLEGE OF MEDICINE   (United States)

^b Department of Environmental and Radiological Health Sciences   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

MEMBRANE LIPID;

ARTICLE; BACTERIAL GROWTH; BACTERIAL VIRULENCE; BACTERIUM MUTANT; GENE DISRUPTION; GENE INSERTION; LIPID TRANSPORT; LIPOGENESIS; MOUSE; NONHUMAN; PRIORITY JOURNAL; TUBERCULOSIS;

ANIMALS; DNA TRANSPOSABLE ELEMENTS; FEMALE; GENES, BACTERIAL; LIPIDS; LIVER; LUNG; MICE; MICE, INBRED C57BL; MUTAGENESIS; MYCOBACTERIUM TUBERCULOSIS; SPLEEN; TUBERCULOSIS; VIRULENCE;

EID: 0033523971     PISSN: 00280836     EISSN: None     Source Type: Journal    
DOI: 10.1038/47042     Document Type: Article

References (23)

1. World Health Organization, World Health Report 1999 http://www.who.int/whr/1999/en/report.htm (1999).
2. Garay, S. M. in Tuberculosis (eds Rom, W. N. & Garay, S. M.) 373-412 (Little, Brown and Company, Boston, 1996).
3. Brennan, P. J. & Nikaido, H. The envelope of mycobactria. Annu. Rev. Biochem. 64, 29-63 (1995).
4. Kolattukudy, P. E., Fernandes, N. D., Azad, A. K., Fitzmaurice, A. M. & Sirakova, T. D. Biochemistry and molecular genetics of cell-wall lipid biosynthesis in mycobacteria. Mol. Microbiol. 24, 263-270 (1997).
5. Hensel, M. et al. Simultaneous identification of bacterial virulence genes by negative selection. Science 269, 400-403 (1995).
6. Chiang, S. L. & Mekalanos, J. J. Use of signature-tagged transposon mutagenesis to identify Vibrio cholerae genes critical for colonization. Mol. Microbiol. 27, 797-805 (1998).
7. Mei, J. M., Nourbakhsh, F., Ford, C. W. & Holden, D. W. Identification of Staphylococcus aureus virulence genes in a murine model of bacteraemia using signature-tagged mutagenesis. Mol. Microbiol. 26, 399-407 (1997).
8. Azad, A. K., Sirakova, T. D., Fernandes, N. D. & Kolattukudy, P. E. Gene knockout reveals a novel gene cluster for the synthesis of a class of cell wall lipids unique to pathogenic mycobacteria. J. Biol. Chem. 272, 16741-16745 (1997).
9. Mathur, M. & Kolattukudy, P. E. Molecular cloning and sequencing of the gene for mycocerosic acid synthase, a novel fatty acid elongating multifunctional enzyme, from Mycobacterium tuberculosis var. bovis Bacillus Calmette-Guerin. J. Biol. Chem. 267, 19388-19395 (1992).
10. Azad, A. K., Sirakova, T. D., Rogers, L. M. & Kolattukudy, P. E. Targeted replacement of the mycocerosic acid synthase gene in Mycobacterium bovis BCG produces a mutant that lacks mycosides. Proc. Natl Acad. Sci. USA 93, 4787-4792 (1996).
11. Cole, S. T. et al. Deciphering the biology of Mycobactrium tuberculosis from the complete genome sequence [see comments]. Nature 393, 537-544 (1998).
12. Fitzmaurice, A. M. & Kolattukudy, P. E. An acyl-CoA synthase (acoas) gene adjacent to the mycocerosic acid synthase (mas) locus is necessary for mycocerosyl lipid synthesis in Mycobacterium tuberculosis var. bovis. BCG. J. Biol. Chem. 273, 8033-8039 (1998).
13. Fitzmaurice, A. M. & Kolattukudy, P. E. Open reading frame 3, which is adjacent to the mycocerosic acid synthase gene, is expressed as an acyl coenzyme A synthase in Mycobacterium bovis BCG. J. Bacteriol. 179, 2608-2615 (1997).
14. Bystrykh, L. V. et al. Production of actinorhodin-related "blue pigments" by Streptomyces coelicolor A3(2). J. Bacteriol. 178, 2238-2244 (1996).
15. Pierce, C. H. & Dubos, R. J. Differential characteristics in vitro and in vivo of several substrains of BCG. II. Morphologic characteristics in vitro and in vivo. Am. Rev. Tuberc. Pulm. Dis. 74, 667-682 (1956).
16. Middlebrook, G., Dubos, R, J. & Pierce, C. Virulence and morphological characteristics of mammalian tubercle bacilli. J. Exp. Med. 86, 175-183 (1947).
17. Rainwater, D. L. & Kolattukudy, P. E. Synthesis of mycocerosic acids from methylmalonyl coenzyme A by cell-free extracts of Mycobacterium tuberculosis var. bovis BCG. J. Biol. Chem. 258, 2979-2985 (1983).
18. Hunter, S. W. & Brennan, P. J. Further specific extracellular phenolic glycolipid antigens and a related diacylphthiocerol from Mycobacterium leprae. J. Biol. Chem. 258, 7556-7562 (1983).
19. Besra, G. S. & Chatterjee, D. in Tuberculosis: Pathogenesis, Protection, and Control (ed. Bloom, B. R.) 285-306 (American Society for Microbiology, Washington DC, 1994).
20. Bardarov, S. et al. Conditionally replicating mycobacteriophages: a system for transposon delivery to Mycobacterium tuberculosis. Proc. Natl Acad. Sci. USA 94, 10961-10966 (1997).
21. Ochman, H., Gerber, A. S. & Hartl, D. L. Genetic applications of an inverse polymerase chain reaction. Genetics 120, 621-623 (1988).
22. Folch, J., Lees, M. & Stanley, G. H. S. A simple method for the isolation and purification of total lipids from animal tissues. J. Biol. Chem. 226, 497-509 (1957).
23. McAdam, R. A. et al. In vivo growth characteristks of leucine and meihionine auxotrophic mutants of Mycobacterium bovis BCG generated by transposon mutagenesis. Infect. Immun. 63, 1004-1012 (1995).