Genetic and biochemical characterization of a novel umuD mutation: Insights into a mechanism for UmuD self-cleavage

Journal of Bacteriology

Volumn 183, Issue 1, 2001, Pages 347-357

  Sutton, M D ^a   Kim, M ^a   Walker, G C ^a  

^a MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARGININE; DNA POLYMERASE; LEUCINE; LYSINE; RECA PROTEIN; REPRESSOR PROTEIN; SERINE; SINGLE STRANDED DNA; UMUD PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; CRYSTAL STRUCTURE; ESCHERICHIA COLI; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEGRADATION; SITE DIRECTED MUTAGENESIS;

ALLELES; AMINO ACID SEQUENCE; BACTERIAL PROTEINS; COLD; DNA, SINGLE-STRANDED; DNA-DIRECTED DNA POLYMERASE; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; NUCLEOPROTEINS; PROTEIN CONFORMATION; REC A RECOMBINASES; RECOMBINATION, GENETIC; SEQUENCE ALIGNMENT; SOS RESPONSE (GENETICS);

ESCHERICHIA COLI; NEGIBACTERIA; PROKARYOTA;

EID: 0035185616     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.183.1.347-357.2001     Document Type: Article

References (71)

1. Dominant negative umuD mutations decreasing RecA-mediated cleavage suggest roles for intact UmuD in modulation of SOS mutagenesis
2. Linkage map of Escherichia coli K12, edition 10: The traditional map
3. - recombination by the mutagenesis complex UmuD'C
4. Purification of a soluble UmuD'C complex from Escherichia coli. Cooperative binding of UmuD'C to single-stranded DNA
5. UmuD mutagenesis protein of Escherichia coli: Overproduction, purification, and cleavage by RecA
6. Novel mechanism of cell division inhibition associated with the SOS response in Escherichia coll
7. Prophage phi 80 is induced in Eschefichia coli K12 recA430
8. New recA mutations that dissociate the various RecA protein activities in Escherichia coli provide evidence for an additional role for RecA protein in UV mutagenesis
9. Proteins required for ultraviolet light and chemical mutagenesis. Identification of the products of the umuC locus of Escherichia coli
10. Dimerization of the UmuD' protein in solution and its implications for regulation of SOS mutagenesis
11. Identification of additional genes belonging to the LexA regulon in Escherichia coli
12. Genetic requirements and mutational specificity of the Escherichia coli SOS mutator activity
13. Visualization of two binding sites for the Escherichia coli UmuD'(2)C complex (DNA pol V) on RecA-ssDNA filaments
14. Targeting of the UmuD, UmuD', and MucA' mutagenesis proteins to DNA by RecA protein
15. Affinity chromatography of RecA protein and RecA nucleoprotein complexes on RecA protein-agarose columns
16. Novel DNA polymerases offer clues to the molecular basis of mutagenesis
17. Human and mouse homologs of Escherichia coli DinB (DNA polymerase W), members of the UmuC/DinB superfamily
18. Analysis of the region between amino acids 30 and 42 of intact UmuD by a monocysteme approach
19. Activity of the Hsp70 chaperone complex - DnaK, DnaJ and GrpE - In initiating phage lambda DNA replication by sequestering and releasing lambda P protein
20. Bridging the gap: A family of novel DNA polymerases that replicate faulty DNA
21. Specific in vivo protem-protein interactions between Escherichia coli SOS mutagenesis proteins
22. Isolation and characterization of mutants of Escherichia coli deficient in induction of mutations by ultraviolet light
23. Functional domains of Escherichia coli recA protein deduced from the mutational sites in the gene
24. DNA-damaging agents stimulate gene expression at specific loci in Eschenchia coli
25. Identification of the amino acid substitutions in two mutant forms of the recA protein from Escherichia coli: recA441 and recA629
26. Differential cleavage of LexA and UmuD mediated by recA Pro67 mutants: Implications for common LexA and UmuD binding sites on RecA
27. Biochemistry of homologous recombination in Escherichia coli
28. Identification of a DinB/UmuC homolog in the archeon Sulfolobus solfataricus
29. Characterization of the umu-complementing operon from R391
30. A monocysteine approach for probing the structure and interactions of the UmuD protein
31. Interactions of Escherichia coli UmuD with activated RecA analyzed by cross-linking UmuD monocysteine derivatives
32. LexA cleavage and other self-processing reactions
33. Mechanism of specific LexA cleavage: Autodigestion and the role of RecA coprotease
34. Cleavage of the Escherichia coli lexA protein by the recA protease
35. Cold sensitvity induced by overproduction of UmuDC in Escherichia coli
36. Intermolecular cleavage by UmuD-like mutagenesis proteins
37. Regulation of UmuD cleavage: Role of the amino-terminal tail
38. Novel Escherichia coli umuD' mutants: Structure-function insights into SOS mutagenesis
39. A role for the umuDC gene products of Escherichia coli in increasing resistance to DNA damage in stationary phase by inhibiting the transition to exponential growth
40. Mutational analysis of the RecA protein L1 region identifies this area as a probable part of the co-protease substrate binding site
41. RecA-mediated cleavage activates UmuD for mutagenesis: Mechanistic relationship between transcriptional derepression and posttranslational activation
42. General recombination: Functions and structure of RecA protein
43. Mutations affecting the ability of the Escherichia coli UmuD' protein to participate in SOS mutagenesis
44. A model for a umuDC-dependent prokaryotic DNA damage checkpoint
45. The genetic requirements for UmuDC-mediated cold sensitivity are distinct from those for SOS mutagenesis
46. Crystal structure of a bacterial signal peptidase in complex with a beta-lactam inhibitor
47. Common protein architecture and binding sites in proteases utilizing a Ser/Lys dyad mechanism
48. Structure of the UmuD' protein and its regulation in response to DNA damage
49. The UmuD' protein filament and its potential role in damage induced mutagenesis
50. umuDC and mucAB operons whose products are required for UV light- and chemical-induced mutagenesis: UmuD, MucA, and LexA proteins share homology
51. Modulation of RecA nucleoprotein function by the mutagenic UmuD'C protein complex
52. Interaction of Escherichia coli RecA protein with LexA repressor. I. LexA repressor cleavage is competitive with binding of a secondary DNA molecule
53. The mutagenesis protein UmuC is a DNA polymerase activated by UmuD', RecA, and SSB and is specialized for translesion replication
54. Nature of the SOS-inducing signal in Escherichia coli. The involvement of DNA replication
55. RecA protein-dependent cleavage of UmuD protein and SOS mutagenesis
56. The appearance of the UmuD'C protein complex in Escherichia coli switches repair from homologous recombination to SOS mutagenesis
57. Specific RecA amino acid changes affect RecA-UmuD'C interaction
58. Uvm mutants of Escherichia coli K12 deficient in UV mutagenesis. I. Isolation of uvm mutants and their phenotypical characterization in DNA repair and mutagenesis
59. The Escherichia coli SOS mutagenesis proteins UmuD and UmuD' interact physically with the replicative DNA polymerase
60. In vitro inhibition of RecA-mediated homologous pairing by UmuD'C proteins
61. Roles of E. coli DNA polymerase IV and V in lesion-targeted and untargeted SOS mutagenesis
62. UmuD'(2)C is an error-prone DNA polymerase, Escherichia coli pol V
63. Inducible DNA repair systems
64. Skiing the black diamond slope: Progress on the biochemistry of translesion DNA synthesis
65. Evidence that the recA441 (tif-1) mutant of Escherichia coli K-12 contains a thermosensitive intragenic suppressor of RecA constitutive protease activity
66. A plethora of lesion-replicating DNA polymerases
67. UmuC mutagenesis protein of Escherichia coli: Purification and interaction with UmuD and UmuD'
68. p-Azidoiodoacetanilide, a new short photocrosslinker that has greater cysteine specificity than p-azidophenacyl bromide and p-azidobromoacetanilide