Novel Escherichia coli umuD' mutants: Structure-function insights into SOS mutagenesis

Journal of Bacteriology

Volumn 180, Issue 17, 1998, Pages 4658-4666

  McLenigan, Mary ^a   Peat, Thomas S ^b,c   Frank, Ekaterina G ^a   McDonald, John P ^a   Gonzalez, Martín ^a   Levine, Arthur S ^a   Hendrickson, Wayne A ^b   Woodgate, Roger ^a  

^a EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH AND HUMAN DEVELOPMENT   (United States)

^b Och Spine at New York Presbyterian Hospitals   (United States)

^c LOS ALAMOS NATIONAL LABORATORY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; MESYLIC ACID METHYL ESTER; PROTEIN UMUD; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL MUTATION; COLORIMETRY; CRYSTAL STRUCTURE; ESCHERICHIA COLI; GENE MUTATION; MUTAGENESIS; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; PROMOTER REGION; STRUCTURE ACTIVITY RELATION;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA;

EID: 0031721529     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.180.17.4658-4666.1998     Document Type: Article

References (41)

1. Bartel, P. L., and S. Fields. 1995. Analyzing protein-protein interactions using two-hybrid system. Methods Enzymol. 254:241-263.
2. Battista, J. R., T. Ohta, T. Nohmi, W. Sun, and G. C. Walker. 1990. Dominant negative umuD mutations decreasing RecA-mediated cleavage suggest roles for intact UmuD in modulation of SOS mutagenesis. Proc. Natl. Acad. Sci. USA 87:7190-7194.
3. Bridges, B. A., and R. Woodgate. 1985. Mutagenic repair in Escherichia coli: products of the recA gene and of the umuD and umuC genes act at different steps in UV-induced mutagenesis. Proc. Natl. Acad. Sci. USA 82:4193-4197.
4. Burckhardt, S. E., R. Woodgate, R. H. Scheuermann, and H. Echols. 1988. UmuD mutagenesis protein of Escherichia coli: overproduction, purification and cleavage by RecA. Proc. Natl. Acad. Sci. USA 85:1811-1815.
5. Donnelly, C. E., and G. C. Walker. 1992. Coexpression of UmuD′ with UmuC suppresses the UV mutagenesis deficiency of groE mutants. J. Bacteriol. 174:3133-3139.
6. Ferentz, A. E., T. Opperman, G. C. Walker, and G. Wagner. 1997. Dimerization of the UmuD′ protein in solution and its implications for regulation of SOS mutagenesis. Nat. Struct. Biol. 4:979-983.
7. Fields, S., and O. K. Song. 1989. A novel genetic system to detect protein-protein interactions. Nature 340:245-246.
8. Fijalkowska, I. J., R. L. Dunn, and R. M. Schaaper. 1997. Genetic requirements and mutational specificity of the Escherichia coli SOS mutator activity. J. Bacteriol. 179:7435-7445.
9. Frank, E. G., D. G. Ennis, M. Gonzalez, A. S. Levine, and R. Woodgate. 1996. Regulation of SOS mutagenesis by proteolysis. Proc. Natl. Acad. Sci. USA 93:10291-10296.
10. Frank, E. G., M. Gonzalez, D. G. Ennis, A. S. Levine, and R. Woodgate. 1996. In vivo stability of the Umu mutagenesis proteins: a major role for RecA. J. Bacteriol. 178:3550-3556.
11. Frank, E. G., J. Hauser, A. S. Levine, and R. Woodgate. 1993. Targeting of the UmuD, UmuD′ and MucA′ mutagenesis proteins to DNA by RecA protein. Proc. Natl. Acad. Sci. USA 90:8169-8173.
12. Friedberg, E. C., G. C. Walker, and W. Siede. 1995. DNA repair and mutagenesis. American Society for Microbiology, Washington, D.C.
13. Guzzo, A., M. H. Lee, K. Oda, and G. C. Walker. 1996. Analysis of the region between amino acids 30 and 42 of intact UmuD by a monocysteine approach. J. Bacteriol. 178:7295-7303.
14. Hill, J., K. A. G. Donald, and D. E. Griffiths. 1991. DMSO-enhanced whole cell yeast transformation. Nucleic Acids Res. 19:5791.
15. Ho, C., O. I. Kulaeva, A. S. Levine, and R. Woodgate. 1993. A rapid method for cloning mutagenic DNA repair genes: isolation of umu-complementing genes from multidrug resistance plasmids R391, R4465, and R471a. J. Bacteriol. 175:5411-5419.
16. Isackson, P. J., and K. P. Bertrand. 1985. Dominant negative mutations in the Tn10 tet repressor: evidence for use of the conserved helix-turn-helix motif in DNA binding. Proc. Natl. Acad. Sci. USA 82:6226-6230.
17. Ho, H., Y. Fukuda, K. Murata, and A. Kimura. 1983. Transformation of intact yeast cells treated with alkali cations. J. Bacteriol. 153:163-168.
18. Jonczyk, P., and A. Nowicka. 1996. Specific in vivo protein-protein interactions between Escherichia coli SOS mutagenesis proteins. J. Bacteriol. 178: 2580-2585.
19. Kato, T., and Y. Shinoura. 1977. Isolation and characterization of mutants of Escherichia coli deficient in induction of mutations by ultraviolet light. Mol. Gen. Genet. 156:121-131.
20. Kobayashi, M., K. Nagata, and A. Ishihama. 1990. Promoter selectivity of Escherichia coli RNA polymerase: effect of base substitutions in the promoter -35 region on promoter strength. Nucleic Acids Res. 18:7367-7372.
21. Koch, W. H., D. G. Ennis, A. S. Levine, and R. Woodgate. 1992. Escherichia coli umuDC mutants: DNA sequence alterations and UmuD cleavage. Mol. Gen. Genet. 233:443-448.
22. -) mutants of the LexA repressor of Escherichai coli K-12. J. Bacteriol. 170:2163-2173.
23. Miller, J. H. 1972. Experiments in molecular genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
24. Nicholls, A., K. A. Sharp, and B. Honig. 1991. Protein folding and association: insights from the interfacial and thermodynamic properties of hydrocarbons. Proteins 11:281-296.
25. Nohmi, T., J. R. Battista, L. A. Dodson, and G. C. Walker. 1988. RecA-mediated cleavage activates UmuD for mutagenesis: mechanistic relationship between transcriptional derepression and posttranslational activation. Proc. Natl. Acad. Sci. USA 85:1816-1820.
26. Oller, A. R., I. J. Fijalkowska, and R. M. Schaaper. 1993. The Escherichia coli galK2 papillation assay: its specificity and application to seven newly isolated mutator strains. Mutat. Res. 292:175-185.
27. Peat, T. S., E. G. Frank, J. P. McDonald, A. S. Levine, R. Woodgate, and W. A. Hendrickson. 1996. Structure of the UmuD′ protein and its regulation in response to DNA damage. Nature 380:727-730.
28. Peat, T. S., E. G. Frank, J. P. McDonald, A. S. Levine, R. Woodgate, and W. A. Hendrickson. 1996. The UmuD′ protein filament and its potential role in damage induced mutagenesis. Structure 4:1401-1412.
29. Perry, K. L., S. J. Elledge, B. Mitchell, L. Marsh, and G. C. Walker. 1985. umuDC and mucAB operons whose products are required for UV light and chemical-induced mutagenesis: UmuD, MucA, and LexA products share homology. Proc. Natl. Acad. Sci. USA 82:4331-4335.
30. Rajagopalan, M., C. Lu, R. Woodgate, M. O'Donnell, M. F. Goodman, and H. Echols. 1992. Activity of the purified mutagenesis proteins UmuC, UmuD′ and RecA in replicative bypass of an abasic DNA lesion by DNA polymerase III. Proc. Natl. Acad. Sci. USA 89:10777-10781.
31. Schiestl, R. H., and R. D. Gietz. 1989. High efficiency transformation of intact yeast cells using single stranded nucleic acids as a carrier. Curr. Genet. 16:339-346.
32. Sherman, F., G. R. Fink, and J. B. Hicks. 1986. Methods in yeast genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
33. Shinagawa, H., H. Iwasaki, T. Kato, and A. Nakata. 1988. RecA protein-dependent cleavage of UmuD protein and SOS mutagenesis. Proc. Natl. Acad. Sci. USA 85:1806-1810.
34. Smith, C. M., W. H. Koch, S. B. Franklin, P. L. Foster, T. A. Cebula, and E. Eisenstadt. 1990. Sequence analysis of the Salmonella typhimurium LT2 umuDC operon. J. Bacteriol. 172:4964-4978.
35. Steinborn, G. 1978. Uvm mutants of Escherichia coli K12 deficient in UV mutagenesis. I. Isolation of uvm mutants and their phenotypical characterization in DNA repair and mutagenesis. Mol. Gen. Genet. 165:87-93.
36. Sundin, G. W., S. P. Kidambi, M. Ullrich, and C. L. Bender. 1996. Resistance to ultraviolet light in Pseudomonas syringae: sequence and functional analysis of the plasmid-encoded rulAB genes. Gene 177:77-81.
37. Sweasy, J. B., E. M. Witkin, N. Sinha, and V. Roegner-Maniscalco. 1990. RecA protein of Escherichia coli has a third essential role in SOS mutator activity. J. Bacteriol. 172:3030-3036.
38. Thomas, S. M., H. M. Crowne, S. C. Pidsley, and S. G. Sedgwick. 1990. Structural characterization of the Salmonella typhimurium LT2 umu operon. J. Bacteriol. 172:4979-4987.
39. Woodgate, R., and D. G. Ennis. 1991. Levels of chromosomally encoded Umu proteins and requirements for in vivo UmuD cleavage. Mol. Gen. Genet. 229:10-16.
40. Woodgate, R., and A. S. Levine. 1996. Damage inducible mutagenesis: recent insights into the activities of the Umu family of mutagenesis proteins, p. 117-140. In T. Lindahl (ed.), Cancer surveys: genetic instability in cancer, 28th ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
41. Woodgate, R., M. Singh, O. I. Kulaeva, E. G. Frank, A. S. Levine, and W. H. Koch. 1994. Isolation and characterization of novel plasmid-encoded umuC mutants. J. Bacteriol. 176:5011-5021.