Differential cleavage of LexA and UmuD mediated by recA Pro67 mutants: Implications for common LexA and UmuD binding sites on RecA

Journal of Molecular Biology

Volumn 276, Issue 2, 1998, Pages 405-415

  Konola, Jukka T ^a   Guzzo, Angelina ^b   Gow, Joan Beth ^a   Walker, Graham C ^b   Knight, Kendall L ^a  

^a 55 Lake Avenue North ^*  (United States)

^b MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

Author keywords

Coprotease; LexA protein; RecA protein; SOS response; UmuD protein

Indexed keywords

BACTERIAL PROTEIN; MUTANT PROTEIN; RECA PROTEIN; REPRESSOR PROTEIN;

ARTICLE; BINDING SITE; CONTROLLED STUDY; ESCHERICHIA COLI; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEGRADATION; STRUCTURE ACTIVITY RELATION;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0032548822     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.1997.1531     Document Type: Article

References (46)

1. Bagg A., Kenyon C.J., Walker G.C. Inducibility of a gene product required for UV and chemical mutagenesis in Escherichia coli. Proc. Natl Acad. Sci. USA. 78:1981;5749-5753
2. Bailone A., Sommer S., Knezevic J., Dutreix M., Devoret R. A RecA protein mutant deficient in its interaction with the UmuDC complex. Biochimie. 73:1991;479-484
3. Burckhardt S.E., Woodgate R., Scheuermann R.H., Echols H. UmuD mutagenesis proteins of Escherichia coli overproduction, purification, and cleavage by RecA. Proc. Natl Acad. Sci. USA. 85:1988;1811-1815
4. Cox M.M. The RecA protein as a recombinational repair system. Mol. Microbiol. 5:1991;1295-1299
5. Cox M.M. Relating biochemistry to biology how the recombinational repair function of RecA protein is manifested in its molecular properties. Bioessays. 15:1993;617-623
6. Dutreix M., Moreau P.L., Bailone A., Galibert F., Battista J.R., Walker G.C., Devoret R. New recA mutations that dissociate the various RecA protein activities in Escherichia coli provide evidence for an additional role for RecA protein in UV mutagenesis. J. Bacteriol. 171:1989;2415-2423
7. Dutreix M., Burnett B., Bailone A., Radding C.M., Devoret R. A partially deficient mutant, recA1730, that fails to form normal nucleoprotein filaments. Mol. Gen. Genet. 232:1992;489-497
8. Egelman E.H., Stasiak A. Electron microscopy of RecA-DNA complexes two different states, their functional significance and relation to the solved crystal structure. Micron. 24:1993;309-324
9. Ennis D.G., Ossanna N., Mount D.W. Genetic separation and repressor cleavage. J. Bacteriol. 171:1989;2533-2541
10. Ennis D.G., Levine A.S., Koch W.H., Woodgate R. Analysis of recA mutants with altered SOS function. Mutat. Res. 336:1995;39-48
11. Frank E.G., Hauser J., Levine A.S., Woodgate R. Targeting of the UmuD, UmuD′, and MucA′ mutagenesis proteins to DNA by RecA protein. Proc. Natl Acad. Sci. USA. 90:1993;8169-8173
12. Frank E.G., Ennis D.G., Gonzalez M., Levine A.S., Woodgate R. Regulation of SOS mutagenesis by proteolysis. Proc. Natl Acad. Sci. USA. 93:1996;10291-10296
13. Friedberg E.C., Walker G.C., Siede W. SOS Responses and DNA Damage Tolerance. Mutagenesis in Prokaryotes. DNA Repair and Mutagenesis. 1995;407-522 American Society for Microbiology, Washington DC
14. Hanahan D. Studies on transformation of Escherichia coli with plasmids. J. Mol. Biol. 166:1983;557-580
15. Harmon F.G., Rehrauer W.M., Kowalczykowski S.C. Interaction of Escherichia coli RecA protein with LexA repressor. II. Inhibition of DNA strand exchange by the uncleavable LexA S119A repressor argues that recombination and SOS induction are competitive processes. J. Biol. Chem. 271:1996;23874-23883
16. Hauser J., Levine A.S., Ennis D.G., Chumakov K.M., Woodgate R. The enhanced mutagenic potential of the MucAB proteins correlates with the highly efficient processing of the MucA protein. J. Bacteriol. 174:1992;6844-6851
17. Ishimori K., Sommer S., Bailone A., Takahashi M., Cox M.M., Devoret R. Characterization of a mutant RecA protein that facilitates homologous genetic recombination but not recombinational DNA repair recA423. J. Mol. Biol. 264:1996;696-712
18. Konola J.T., Logan K.M., Knight K.L. Functional characterization of residues in the P-loop motif of the RecA protein ATP binding site. J. Mol Biol. 237:1994;20-34
19. Konola J.T., Nastri H.G., Logan K.M., Knight K.L. Mutations at Pro67 in the RecA protein P-loop motif differentially modify coprotease function and separate coprotease from recombination activities. J. Biol. Chem. 270:1995;8411-8419
20. Kowalczykowski S.C., Dixon D.A., Eggleston A.K., Lauder S.D., Rehrauer W.M. Biochemistry of homologous recombination in Escherichia coli. Microbiol. Rev. 58:1994;401-465
21. Leahy M.C., Radding C.M. Topography of the interaction of RecA protein with single-stranded deoxyribonucleotides. J. Biol. Chem. 261:1986;6954-6960
22. Lee M.H., Walker G.C. Interactions of Escherichia coli UmuD with activated RecA analyzed by crosslinking UmuD monocysteine derivatives. J. Bacteriol. 178:1996;7285-7294
23. Little J.W. The SOS regulatory system control of its state by the level of RecA protease. J. Mol. Biol. 167:1983;791-808
24. Little J.W. Autodigestion of the lexA and phage λ repressors. Proc. Natl Acad. Sci. USA. 81:1984;1375-1379
25. Little J.W. Mechanism of specific LexA cleavage autodigestion and the role of RecA coprotease. Biochimie. 73:1991;411-422
26. Little J.W. LexA cleavage and other self-processing reactions. J. Bacteriol. 175:1993;4943-4950
27. Logan K.M., Knight K.L. Mutagenesis of the P-loop motif in the ATP binding site of the RecA protein from Escherichia coli. J. Mol. Biol. 232:1993;1048-1059
28. Lowry O.H., Rosebrough N.J., Farr A.L., Randall R.J. Protein measurement with Folin phenol reagent. J. Biol. Chem. 193:1951;265-275
29. Nohmi T., Battista J.R., Dodson L.A., Walker G.C. RecA-mediated cleavage activates UmuD for mutagenesis mechanistic relationship between transcriptional derepression and posttranslational activation. Proc. Natl Acad. Sci. USA. 85:1988;1816-1820
30. Ogawa H., Ogawa T. General recombination functions and structure of RecA protein. Advan. Biophys. 21:1986;135-148
31. Opperman T., Murli S., Walker G.C. The genetic requirements for UmuDC-mediated cold sensitivity are distinct from those for SOS mutagenesis. J. Bacteriol. 178:1996;4400-4411
32. Rehrauer W.M., Kowalczykowski S.C. The DNA binding site(s) of the Escherichia coli RecA protein. J. Biol. Chem. 271:1996;11996-12002
33. Rehrauer W.M., Lavery P.E., Plamer E.L., Singh R.N., Kowalczykowski S.C. Interaction of Escherichia coli RecA protein with LexA repressor. I. LexA repressor cleavage is competitive with binding of a secondary DNA molecule. J. Biol. Chem. 271:1996;23865-23873
34. Roca A.I., Cox M.M. RecA protein structure, function, and role in recombinational DNA repair. Prog. Nucl. Acid Res. Mol. Biol. 56:1997;129-222
35. Sassanfar M., Roberts J.W. Nature of the SOS-inducing signal in Escherichia coli. The involvement of DNA replication. J. Mol. Biol. 212:1990;79-96
36. Shepley D.P., Little J.W. Mutant LexA proteins with specific defects in autodigestion. Proc. Natl Acad. Sci. USA. 93:1996;11528-11533
37. Shinagawa H., Iwasaki H., Kato T., Nakata A. RecA protein-dependent cleavage of UmuD protein and SOS mutagenesis. Proc. Natl Acad. Sci. USA. 85:1988;1806-1810
38. Slezarikova V., Masek F., Fridrichova I., Pirsel M., Sedliakova M. Constitutive increase of RecA protein its influence on pyrimidine dimer excision and survival of UV-irradiated Escherichia coli. Mutat. Res. 362:1996;61-64
39. Sommer S., Bailone A., Devoret R. The appearance of the UmuD′C protein complex in Escherichia coli switches repair from homologous recombination to SOS mutagenesis. Mol. Microbiol. 10:1993;963-971
40. Stole E., Bryant F.R. Reengineering the nucleotide cofactor specificity of the RecA protein by mutation of aspartic acid 100. J. Biol. Chem. 271:1996;18326-18328
41. Story R.M., Steitz T.A. Structure of the RecA protein-ADP complex. Nature. 355:1992;374-376
42. Story R.M., Weber I.T., Steitz T.A. The structure of the E. coli RecA protein monomer and polymer. Nature. 355:1992;318-325
43. Thliveris A.T., Mount D.W. Genetic identification of the DNA binding domain of Escherichia coli LexA protein. Proc. Natl Acad. Sci. USA. 89:1992;4500-4504
44. Walker G.C. SOS-regulated proteins in translesion DNA synthesis and mutagenesis. Trends Biochem. Sci. 20:1995;416-420
45. Woodgate R., Rajagopalan M., Lu C., Echols H. UmuC mutagenesis protein of Escherichia coli purification and interaction with UmuD and UmuD′ Proc. Natl Acad. Sci. USA. 86:1989;7301-7305
46. Yu X., Egelman E.H. The LexA repressor binds within the deep helical groove of the activated RecA filament. J. Mol. Biol. 231:1993;29-40