Characterization of early immunological responses in primary cultures of differentially activated human peripheral mononuclear cells

Journal of Immunological Methods

Volumn 247, Issue 1-2, 2001, Pages 131-139

  Kruse, N ^a   Moriabadi, N F ^a   Toyka, K V ^a   Rieckmann, P ^a  

^a UNIVERSITY HOSPITAL WÜRZBURG   (Germany)

Author keywords

Cytokine mRNA; Cytokine protein; Human PBMCs; RT PCR

Indexed keywords

BETA ACTIN; CYTOKINE; GAMMA INTERFERON; INTERLEUKIN 10; INTERLEUKIN 2; INTERLEUKIN 4; MESSENGER RNA; TUMOR NECROSIS FACTOR ALPHA;

ARTICLE; CONTROLLED STUDY; CORRELATION FUNCTION; HUMAN; HUMAN CELL; IMMUNE RESPONSE; IMMUNITY; LYMPHOCYTE ACTIVATION; LYMPHOCYTE CULTURE; LYMPHOCYTE PROLIFERATION; MONONUCLEAR CELL; PRIORITY JOURNAL; PROTEIN EXPRESSION; QUANTITATIVE ASSAY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

CELL DIVISION; CELLS, CULTURED; CYTOKINES; HUMANS; INTERLEUKIN-12; INTERLEUKIN-4; LEUKOCYTES, MONONUCLEAR; RNA, MESSENGER;

EID: 0035182561     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(00)00316-1     Document Type: Article

References (31)

1. Transforming growth factor-beta 1, macrophage inflammatory protein-1 alpha, and interleukin-8 gene expression is lower in stimulated human neonatal compared with adult mononuclear cells
2. Assessment of immunologic status of liver transplant recipients by peripheral blood mononuclear cells in response to stimulation by donor alloantigen
3. Direct stimulation of cytokines (IL-1β, TNF-α, IL-6, IL-2, IFN-γ and GM-CSF) in whole blood. Comparison with isolated PBMC stimulation
4. Myelin basic protein
5. Real-time quantitative RT-PCR after laser-assisted cell picking
6. A novel method for real time quantitative RT-PCR
7. Analysis of cytokine mRNA and DNA, detection and quantitation by competitive polymerase chain reaction
8. Interferon-γ production in antigen specific T cell response, quantitation of specific mRNA and secreted protein
9. An analysis of interferon γ, IL-4, IL-5 and IL-10 production by ELISPOT and quantitative reverse transcriptase-PCR in human Peyer's patches
10. Real time quantitative PCR
11. Detection of intracellular antigen-specific cytokines in human T cell populations
12. Quantification of human interleukin 18 mRNA expression by competitive reverse transcriptas polymerase chain reaction
13. Expression of genes for proinflammatory cytokines in alveolar macrophages during propofol and isoflurane anesthesia
14. Quantification of cytokine mRNA expression by RT-PCR in samples of previously frozen blood
15. Variations in cytokine mRNA expression during normal human pregnancy
16. Increased transforming growth factor-β, interleukin-4, and interferon-γ in multiple sclerosis
17. The kinetics and stimulant dependence of cytokine production by blood and bronchoalveolar lavage T cells evaluated at the single cell level
18. A sensitive ELISPOT assay to detect low-frequency human T lymphocytes
19. Quantification of low-copy transcripts by continuous SYBR Green I monitoring during amplification
20. Production of type-1 and type-2 cytokines by peripheral blood mononuclear cells of psoriasis patients
21. Augmented expression of tumour necrosis factor-α and lymphotoxin in mononuclear cells in multiple sclerosis and optic neuritis
22. Autoreactive T lymphocytes in multiple sclerosis determined by antigen-induced secretion of interferon gamma
23. Critical influence of the cytokine orchestration on the outcome of myelin antigen-specific T-cell autoimmunity in experimental autoimmune encephalomyelitis and multiple sclerosis
24. Quantification of murine cytokine mRNAs using real time quantitative reverse transcriptase PCR
25. Tumor necrosis factor-α messenger RNA expression in patients with relapsing-remitting multiple sclerosis is associated with disease activity
26. The Th1/Th2 paradigm
27. Kinetics of cytokine and NFAT gene expression in human interleukin-2-dependent T lymphoblasts stimulated via T-cell receptor
28. CD14
29. Absence of CHR.4.63; original antigenic sin″ in autoimmunity provides an unforeseen platform for immune therapy
30. Spontaneous regression of primary autoreactivity during chronic progression of experimental autoimmune encephalomyelitis and multiple sclerosis
31. Monocyte-regulated IFN-γ production in human T cells involves CD2 signaling