Use of direct-infusion electrospray mass spectrometry to guide empirical development of improved conditions for expression of secondary metabolites from actinomycetes

Applied and Environmental Microbiology

Volumn 67, Issue 1, 2001, Pages 377-386

  Zahn, J A ^a   Higgs, R E ^a   Hilton, M D ^a  

^a ELI LILLY AND COMPANY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ION;

ACTINOBACTERIA; ARTICLE; BACTERIAL METABOLISM; BACTERIUM CULTURE; CHEMICAL COMPOSITION; FERMENTATION; LIGHT SCATTERING; MASS SPECTROMETRY; METABOLITE; MICROBIAL GROWTH; NONHUMAN; PROTEIN ANALYSIS; PROTEIN DETERMINATION; PROTEIN EXPRESSION; SOLID PHASE EXTRACTION;

ACTINOMYCETALES; CULTURE MEDIA; FERMENTATION; FILTRATION; REPRODUCIBILITY OF RESULTS; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION;

ACTINOBACTERIA (CLASS); BACTERIA (MICROORGANISMS); UNCULTURED ACTINOMYCETE;

EID: 0035167680     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.67.1.377-386.2001     Document Type: Article

References (41)

1. Characterization of metabolites in intact Streptomyces citricolor culture supernatants using high-resolution nuclear magnetic resonance rand directly coupled high-pressure liquid chromatography-nuclear magnetic resonance spectroscopy
2. Production and properties of three pectinolytic activities produced by Aspergillus niger in submerged and solid-state fermentation
3. A Streptomyces griseus gene (sga A) suppresses the growth disturbance caused by high osmolarity and high concentration of A-factor during early growth
4. New perspectives in lead generation. II. Evaluating molecular diversity
5. Production of secondary metabolites by solid-state fermentation
6. What's in a name? - Microbial secondary metabolism
7. Secondary metabolites as chemical signals for cellular differentiation
8. Pharmaceutically active secondary metabolites of microorganisms
9. Biomass estimation in solid-state fermentation: Manual biochemical methods
10. Biomass estimation in solid-state fermentation: On-line measurements
11. The evaporative light scattering detector: A universal instrument for non-volatile solutes in LC rand SFC
12. Cell-cell communication in gram-positive bacteria
13. Detection of novel secondary metabolites
14. Solution-phase library generation: Methods and applications in drug discovery
15. Bioactive agents from natural sources: Trends in discovery and application
16. Influence of various parameters on the response factors of the evaporative light scattering detector for a number of non-volatile compounds
17. Mutants blocked in streptomycin production in Streptomyces griseus: The role of A-factor
18. Solid-state fermentation, part 1
19. Solid-state fermentation, part 2
20. Rapid method to estimate the presence of secondary metabolites in microbial extracts
21. Autoregulatory factors and communication in actinomycetes
22. Solid-state fermentation for cephalosporin production by Streptomyces clavuligerus and Cephalosporium acremonium
23. A method for quantitatively differentiating crude natural extracts using high-performance liquid chromatography-electrospray mass spectrometry
24. Biomolecular-chemical screening: A novel screening approach for the discovery of biologically active secondary metabolites
25. Modular peptide synthetases involved in nonribosomal peptide synthesis
26. Pamamycin: A new antibiotic and stimulator of aerial mycelia formation
27. An efficient approach for screening minimal PKS genes from Streptomyces
28. Discovery of novel secondary metabolites from fungi - Is it really a random walk through a random forest?
29. NMR screening in drag discovery
30. Biochemical engineering aspects of solid-state fermentation
31. Production of antifungal antibiotic, iturin in a solid-state fermentation by Bacillus subtilis nb22 using wheat bran as a substrate
32. Production and industrial importance of secondary products of metabolism
33. Effect of butyrolactone I on the producing fungus, Aspergillus terreus
34. Purification and characterization of glucoamylase produced by Aspergillis niger in solidstate fermentation
35. A study of iterative type II polyketide synthases, using bacterial genes clones from soil DNA: A means to access and use genes from uncultured microorganisms
36. Archaeal ubiquity
37. Growth estimation of Aspergillus oryzae cultured on solid media
38. Tetracycline production with sweet potato residue by solid-state fermentation
39. Screening microbial metabolites for new drags: Theoretical and practical issues
40. Novel approaches to metabolite screening