A functional genomics strategy that uses metabolome data to reveal the phenotype of silent mutations

Nature Biotechnology

Volumn 19, Issue 1, 2001, Pages 45-50

  Raamsdonk, Léonie M ^a   Teusink, Bas ^a,b   Broadhurst, David ^c   Zhang, Nianshu ^d   Hayes, Andrew ^d   Walsh, Michael C ^a,e   Berden, Jan A ^a   Brindle, Kevin M ^f   Kell, Douglas B ^c   Rowland, Jem J ^c   Westerhoff, Hans V ^a,g   Van Dam, Karel ^a   Oliver, Stephen G ^d  

^a UNIVERSITY OF AMSTERDAM   (Netherlands)

^b TNO   (Netherlands)

^c ABERYSTWYTH UNIVERSITY   (United Kingdom)

^d UNIVERSITY OF MANCHESTER   (United Kingdom)

^e Heineken Technical Services BV   (Netherlands)

^f UNIVERSITY OF CAMBRIDGE   (United Kingdom)

^g VU UNIVERSITY AMSTERDAM   (Netherlands)

Author keywords

Co response analysis; Functional genomics; Metabolic control analysis; Metabolome; Phenotype analysis; Saccharomyces cerevisiae; Silent mutations; Yeast

Indexed keywords

ARTICLE; CONTROLLED STUDY; GENE DELETION; GENE MUTATION; GENOME; METABOLIC REGULATION; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; SACCHAROMYCES CEREVISIAE; SILENT GENE;

ADENINE NUCLEOTIDES; CLUSTER ANALYSIS; ENERGY METABOLISM; GENOME, FUNGAL; GENOMICS; GENOTYPE; HEXOSEPHOSPHATES; MUTATION; PHENOTYPE; PYRUVATES; SACCHAROMYCES CEREVISIAE;

FUNGI; MYXOGASTRIA; SACCHAROMYCES CEREVISIAE;

EID: 0035160371     PISSN: 10870156     EISSN: None     Source Type: Journal    
DOI: 10.1038/83496     Document Type: Article

References (54)

1. Functional genomics: It's all how you read it
2. Genomic biology
3. From DNA sequence to biological function
4. Guilt-by-association goes global
5. Systematic functional analysis of the yeast genome
6. Life with 6000 genes
7. Metabolic control analysis as a tool in the elucidation of the function of novel genes
8. Functional analysis of the genes of yeast chromosome V by genetic footprinting
9. Quantitative analysis of yeast gene function using competition experiments in continuous culture
10. Genomic profiling of drug sensitivities via induced haploinsufficiency
11. Taking enzyme kinetics out of control; putting control into regulation
12. Co-response analysis: A new experimental strategy for metabolic control analysis
13. Composite control of cell function: Metabolic pathways behaving as single control units
14. How to recognize monofunctional units in a metabolic system
15. Yeast as a navigational aid in genome analysis
16. Snapshots of systems: Metabolic control analysis and biotechnology in the post-genomic era
17. Yeast 6-phosphofructo-2-kinsae: Sequence and mutant
18. Identification and cloning o yea phosphofructokinase 2
19. The yeast FBP26 gene codes for a fructose-2,6-bisphosphatase
20. Cloning of a second gene encoding 6-phosphofructo-2-kinase in yeast, and its characterization of mutant strains without fructose-2,6-bisphosphate
21. Mammalian 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase: A bifunctional enzyme that controls glycolysis
22. Fructose 2,6-bisphosphate
23. A kinetic study of pyrophosphate fructose-6-phosphate phosphotransferase from potato tubers. Application to a microassay of fructose 2,6-bisphosphate
24. Suitability of replacement markers for functional analysis studies n Saccharomyces cerevisiae
25. New heterologous modules for c ass ca or PCR-based gene disruptions in Saccharomyces cerevisiae
26. Genetic engineering of industrial yeasts
27. Ethanol production using nuclear petite yeast mutants
28. Diffuse reflectance absorbance spectroscopy taking in chemometrics (DRASTIC). A hyperspectral FT-IR-based approach to rapid screening for metabolite overproduction
29. Electrospray: Principles and practice
30. Direct coupling of chromatographic separations to NMR spectroscopy
31. The control of flux
32. Control analysis of metabolic systems
33. Metabolic control theory - Its role in microbiology and biotechnology
34. From genome to cellular phenotype - A role for metabolic flux analysis?
35. Linear theory of enzymatic chains; its application for the analysis of the crossover theorem and of the glycolysis of human erythrocytes
36. Redundancy reveals drugs in action
37. A test case of correlation metric construction of a reaction pathway from measurements
38. Functional characterization of the S. cerevisiae genome by gene deletion and parallel analysis
39. Ethidium bromide induced mutation of yeast mitochondria: Compete transformation of cells into respiratory deficient non-chromosomal petites
40. A rapid and reliable method for metabolite extraction in yeast using boiling buffered ethanol
41. Determination of control coefficients intact metabolic systems
42. On mass spectrometer instrument standardization and interlaboratory calibration transfer using neural networks
43. Rapid identification of urinary tract infection bacteria using hyperspectral, whole organism fingerprinting and artificial neural networks
44. Use of canonical variates in differentiation of bacteria by pyrolysis gas-liquid chromatography
45. Interpretation of sets of mass spectra by discriminant-analysis and graphical rotation
46. Construction of a set of convenient Saccharomyces cerevisiae strains that are isogenic to S288C